In-house validation and quality control of real-time PCR methods for GMO detection: A practical approach

Developments in Biologicals

Volumn 126, Issue , 2006, Pages 79-86

  Ciabatti, I ^a   Froiio, A ^a   Gatto, F ^a   Amaddeo, D ^a   Marchesi, U ^a  

^a ISTITUTO ZOOPROFILATTICO SPERIMENTALE DELLE REGIONI LAZIO E TOSCANA   (Italy)

Author keywords

Genetically modified organism; Quality control; Real time PCR; Validation

Indexed keywords

CONFERENCE PAPER; GENETICALLY MODIFIED FOOD; GENETICS; QUALITY CONTROL; REPRODUCIBILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SOYBEAN; STANDARD; TRANSGENIC PLANT;

FOOD, GENETICALLY MODIFIED; PLANTS, GENETICALLY MODIFIED; QUALITY CONTROL; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SOYBEANS;

EID: 33748605846     PISSN: 14246074     EISSN: None     Source Type: Book Series    
DOI: None     Document Type: Conference Paper

References (9)

1. Regulation (EC) n° 1829/2003 of the European Parliament and of the Council of 22 September 2003 on genetically modified food and feed. OJ L 268, 18/10/2003, pp 1-23.
2. Consideration of the methods for the detection and identification of foods derived from biotechnology - General approach and criteria for the methods CODEX CX/MAS 05/26/9 (January 2005).
3. Definition of minimum performance requirements for analytical methods of GMO testing - ENGL 25/01/2005.
4. Draft European Standard prEN ISO 24276:2002.
5. Draft European Standard ISO/FDIS 21570:2003(E).
6. OIE Manual of diagnostic tests and vaccines for terrestrial animals, 5th edition, 2004.
7. Hübner P, Waiblinger HU, Pietsch K, Brodmann P: Validation of PCR methods for quantification of genetically modified plants in food. J AOAC Int 2001:84,1855-1864.
8. Schwarz G, Bäumler S, Block A, Felsenstein FG, Wenzel G: Determination of detection and quantification limits for SNP allele frequency estimation in DNA pools using real-time PCR. NAR 2004;32:24.
9. Westgard JO: Internal quality control: planning and implementation strategies. Ann Clin Biochem 2003;40:593-611.