Extremely low exposure of a community to severe acute respiratory syndrome coronavirus: False seropositivity due to use of bacterially derived antigens

Journal of Virology

Volumn 80, Issue 18, 2006, Pages 8920-8928

  Leung, D T M ^a   Van Maren, W W C ^a,b   Chan, F K L ^a   Chan, W S ^a   Lo, A W I ^a   Ma, C H ^a   Tam, F C H ^a   To, K F ^a   Chan, P K S ^a   Sung, J J Y ^a   Lim, P L ^a,c  

^a CHINESE UNIVERSITY OF HONG KONG   (Hong Kong)

^b UTRECHT UNIVERSITY   (Netherlands)

^c CHINESE UNIVERSITY OF HONG KONG   (Hong Kong)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ANTIGEN; CELL ANTIGEN; CROSS REACTING ANTIGEN; EPITOPE; IMMUNOGLOBULIN G ANTIBODY; MONOCLONAL ANTIBODY; TISSUE ANTIGEN; VIRUS ANTIBODY;

ADULT; AGED; ANIMAL CELL; ANTIBODY ENGINEERING; ANTIGEN BINDING; ANTIGEN EXPRESSION; ARTICLE; CELL STRAIN; COMMUNITY ASSESSMENT; CONTROLLED STUDY; FALSE POSITIVE RESULT; FEMALE; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; HUMAN TISSUE; MAJOR CLINICAL STUDY; MALE; MASS SCREENING; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN INDUCTION; PROTEIN PROCESSING; PUBLIC HEALTH; SARS CORONAVIRUS; SCREENING TEST; SEROPREVALENCE; SERUM; SEVERE ACUTE RESPIRATORY SYNDROME; VERO CELL; VIRUS NUCLEOCAPSID; VIRUS VIRULENCE;

ANIMALS; ANTIBODIES, VIRAL; ANTIBODY SPECIFICITY; CERCOPITHECUS AETHIOPS; COHORT STUDIES; EPITOPES; FALSE POSITIVE REACTIONS; HONG KONG; HUMANS; IMMUNOGLOBULINS; MASS SCREENING; NUCLEOCAPSID PROTEINS; SARS VIRUS; SEVERE ACUTE RESPIRATORY SYNDROME; TRANSFECTION; VERO CELLS;

BACTERIA (MICROORGANISMS); EUKARYOTA; SARS CORONAVIRUS;

EID: 33748494466     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.00649-06     Document Type: Article

References (34)

1. Chan, P. K. S., M. Ip, K. C. Ng, C. W. Rickjason, A. Wu, N. Lee, T. H. Rainer, G. M. Joynt, J. J. Sung, and J. S. Tam. 2003. Severe acute respiratory syndrome-associated coronavirus infection. Emerg. Infect. Dis. 9:1453-1454.
2. Chan, V. S., K. Y. Chan, Y. Chen, L. L. Poon, A. N. Cheung, B. Zheng, K. H. Chan, W. Mak, H. Y. Ngan, X. Xu, G. Screaton, P. K. Tam, J. M. Austyn, L. C. Chan, S. P. Yip, M. Peiris, U. S. Khoo, and C. L. Lin. 2006. Homozygous L-SIGN (CLEC4M) plays a protective role in SARS coronavirus infection. Nat. Genet. 38:38-46.
3. Che, X. Y., L. W. Qiu, Z. Y. Liao, Y. D. Wang, K. Wen, Y. X. Pan, W. Hao, Y. B. Mei, V. C. Cheng, and K. V. Yuen. 2005. Antigenic cross-reactivity between severe acute respiratory syndrome-associated coronavirus and human coronaviruses 229E and OC43. J. Infect. Dis. 191:2033-2037.
4. Che, X. Y., L. W. Qiu, Y. X. Pan, K. Wen, W. Hao, L. Y. Zhang, Y. D. Wang, Z. Y. Liao, X. Hua, V. C. Cheng, and K. Y. Yuen. 2004. Sensitive and specific monoclonal antibody-based capture enzyme immunoassay for detection of nucleocapsid antigen in sera from patients with severe acute respiratory syndrome. J. Clin. Microbiol. 42:2629-2635.
5. Chien, Y. C., J. Y. Chen, M. Y. Liu, H. I. Yang, M. M. Hsu, C. J. Chen, and C. S. Yang. 2001. Serologic markers of Epstein-Barr virus infection and nasopharyngeal carcinoma in Taiwanese men. N. Engl. J. Med. 345:1877-1882.
6. Chow, P. K., E. E. Ooi, H. K. Tan, K. W. Ong, B. K. Sil, M. Teo, T. Ng, and K. C. Soo. 2004. Healthcare worker seroconversion in SARS outbreak. Emerg. Infect. Dis. 10:249-250.
7. Drosten, C, S. Gunther, W. Preiser, S. van der Werf, H. R. Brodt, S. Becker, H. Rabenau, M. Panning, L. Kolesnikova, R. A. Fouchier, A. Berger, A. M. Burguiere, J. Cinatl, M. Eickmann, N. Escriou, K. Grywna, S. Kramme, J. C. Manuguerra, S. Muller, V. Rickerts, M. Sturmer, S. Vieth, H. D. Klenk, A. D. Osterhaus, H. Schmitz, and H. W. Doerr. 2003. Identification of a novel coronavirus in patients with severe acute respiratory syndrome. N. Engl. J. Med. 348:1967-1976.
8. Herremans, T. M., J. H. Reimerink, A. M. Buisman, T. G. Kimman, and M. P. Koopmans. 1999. Induction of mucosal immunity by inactivated poliovirus vaccine is dependent on previous mucosal contact with live virus. J. Immunol. 162:5011-5018.
9. Ip, M., P. K. S. Chan, N. Lee, A. Wu, T. K. C. Ng, L. Chan, A. Ng, H. M. Kwan, L. Tsang, I. Chu, J. L. K. Cheung, J. J. Y. Sung, and J. S. Tam. 2004. Seroprevalence of antibody to severe acute respiratory syndrome (SARS)-associated coronavirus among health care workers in SARS and non-SARS medical wards. Clin. Infect. Dis. 38:e116-e118.
10. Jeffers, S. A., S. M. Tusell, L. Gillim-Ross, E. M. Hemmila, J. E. Achenbach, G. J. Babcock, W. D. J. Thomas, L. B. Thackray, M. D. Young, R. J. Mason, D. M. Ambrosino, D. E. Wentworth, J. C. Demartini, and K, V. Holmes. 2004. CD209L (L-SIGN) is a receptor for severe acute respiratory syndrome coronavirus. Proc. Natl. Acad. Sci. USA 101:15748-15753.
11. Ksiazek, T. G., D. Erdman, C. S. Goldsmith, S. R. Zaki, T. Peret, S. Emery, S. Tong, C. Urbani, J. A. Comer, W. Lim, P. E. Rollin, S. F. Dowell, A. E. Ling, C. D. Humphrey, W. J. Shieh, J. Guarner, C. D. Paddock, P. Rota, B. Fields, J. DeRisi, J. Y. Yang, N. Cox, J. M. Hughes, J. W. LeDuc, W. J. Bellini, and L. J. Anderson. 2003. A novel coronavirus associated with severe acute respiratory syndrome. N. Engl. J. Med. 348:1953-1966.
12. Kuba, K., Y. Imai, S. Rao, H. Gao, F. Guo, B. Guan, Y. Huan, P. Yang, Y. Zhang, W. Deng, L. Bao, B. Zhang, G. Liu, Z. Wang, M. Chappell, Y. Liu, D. Zheng, A. Leibbrandt, T. Wada, A. S. Slutsky, D. Liu, C. Qin, C. Jiang, and J. M. Penninger. 2005. A crucial role of angiotensin converting enzyme 2 (ACE2) in SARS coronavirus-induced lung injury. Nat. Med. 11:875-879.
13. Lai, T. S. T., T. K. Ng, W. H. Seto, L. Yam, K. I. Law, and J. Chan. 2005. Low prevalence of subclinical severe acute respiratory syndrome-associated coronavirus infection among hospital healthcare workers in Hong Kong. Scand. J. Infect. Dis. 37:500-503.
14. Lau, S. K., P. C. Woo, B. H. Wong, H. W. Tsoi, G. K. Woo, R. W. Poon, K. H. Chan, W. I. Wei, J. S. Peiris, and K. Y. Yuen. 2004. Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in SARS patients by enzyme-linked immunosorbent assay. J. Clin. Microbiol. 42:2884-2889.
15. Leung, D. T. M., F. C. H. Tam, C. H. Ma, P. K. S. Chan, J. L. K. Cheung, H. Niu, J. S. L. Tam, and P. L. Lim. 2004. Antibody response of patients with severe acute respiratory syndrome (SARS) targets the viral nucleocapsid. J. Infect. Dis. 190:379-386.
16. Leung, G. M., P. H. Chung, T. Tsang, W. Lim, S. K. K. Chan, P. Chau, C. A. Donnelly, A. C. Ghani, C. Fraser, S. Riley, N. M. Ferguson, R. M. Anderson, Y. L. Law, T. Mok, T. Ng, A. Fu, P. Y. Leung, J. S. M. Peiris, T. H. Lam, and A. J. Hedley. 2004. SARS-CoV antibody prevalence in all Hong Kong patient contacts. Emerg. Infect. Dis. 10:1653-1656.
17. Liu, W., A. Fontanet, P. H. Zhang, L. Zhan, Z. T. Xin, L. Baril, F. Tang, H. Lv, and W. C. Cao. 2006. Two-year prospective study of the humoral immune response of patients with severe acute respiratory syndrome. J. Infect. Dis. 193:792-795.
18. Marra, M. A., S. J. Jones, C. R. Astell, R. A. Holt, A. Brooks-Wilson, Y. S. Butterfield, J. Khattra, J. K. Asano, S. A. Barber, S. Y. Chan, A. Cloutier, S. M. Coughlin, D. Freeman, N. Girn, O. L. Griffith, S. R. Leach, M. Mayo, H. McDonald, S. B. Montgomery, P. K. Pandoh, A. S. Petrescu, A. G. Robertson, J. E. Schein, A. Siddiqui, D. E. Smailus, J. M. Stott, G. S. Yang, F. Plummer, A. Andonov, H. Artsob, N. Bastien, K. Bernard, T. F. Booth, D. Bowness, M. Drebot, L. Fernando, R. Flick, M. Garbutt, M. Gray, A. Grolla, S. Jones, H. Feldmann, A. Meyers, A. Kabani, Y. Li, S. Normand, U. Stroher, G. A. Tipples, S. Tvler, R. Vogrig, D. Ward, B. Watson, R. C. Brunham, M. Krajden, M. Petric, D. M. Skowronski, C. Upton, and R. L. Roper. 2003. The genome sequence of the SARS-associated coronavirus. Science 300:1399-1404.
19. Nie, Y., G. Wang, X. Shi, H. Zhang, Y. Qiu, Z. He, W. Wang, G. Lian, X. Yin, L. Du, L. Ren, J. Wang, X. He, T. Li, H. Deng, and M. Ding. 2004. Neutralizing antibodies in patients with severe acute respiratory syndrome-associated coronavirus infection. J. Infect. Dis. 190:1119-1126.
20. Peiris, J. S. M., S. T. Lai, L. L. M. Poon, L. Guan, L. Y. C. Yam, W. Lim, J. Nicholls, W. K. S. Yee, W. W. Yan, M. T. Cheung, V. C. C. Cheng, K. H. Chan, D. N. C. Tsang, R. W. H. Yung, T. K. Ng, and K. Y. Yuen. 2003. Coronavirus as a possible cause of severe acute respiratory syndrome. Lancet 361:1319-1325.
21. Perlman, S., and A. A. Dandekar. 2005. Immunopathogenesis of coronavirus infections: implications for SARS. Nat. Rev. Immunol. 5:917-927.
22. Rota, P. A., M. S. Oberste, S. S. Monroe, W. A. Nix, R. Campagnoli, J. P. Icenogle, S. Penaranda, B. Bankamp, K. Maher, M. H. Chen, S. Tong, A. Tamin, L. Lowe, M. Frace, J. L. DeRisi, Q. Chen, D. Wang, D. D. Erdman, T. C. Peret, C. Burns, T. G. Ksiazek, P. E. Rollin, A. Sanchez, S. Liffick, B. Holloway, J. Limor, K. McCaustland, M. Olsen-Rassmussen, R. Fouchier, S. Gunther, A. D. Osterhaus, C. Drosten, M. A. Pallansch, L. J. Anderson, and W. J. Bellini. 2003. Characterization of a novel coronavirus associated with severe acute respiratory syndrome. Science 300:1394-1399.
23. Sun, Z. F., and X. J. Meng. 2004. Antigenic cross-reactivity between the nucleocapsid protein of severe acute respiratory syndrome (SARS) corona-virus and polyclonal antisera of antigenic group I animal coronaviruses: implication for SARS diagnosis. J. Clin. Microbiol. 42:2351-2352.
24. Surjit, M., R. Kumar, R. N. Mishra, M. K. Reddy, V. T. Chow, and S. K. Lal. 2005. The severe acute respiratory syndrome coronavirus nucleocapsid protein is phosphorylated and localizes in the cytoplasm by 14-3-3-mediated translocation. J. Virol. 79:11476-11486.
25. Tse, G. M., K. F. To, P. K. Chan, A. W. Lo, K. C. Ng, A. Wu, N. Lee, H. C. Wong, S. M. Mak, K. F. Chan, D. S. Hui, J. J. Sung, and H. K. Ng. 2004. Pulmonary pathological features in coronavirus associated severe acute respiratory syndrome (SARS). J. Clin. Pathol. 57:260-265.
26. Wang, Y. D., W. Y. Sin, G. B. Xu, H. H. Yang, T. Y. Wong, X. W. Pang, X. Y. He, H. G. Zhang, J. N. Ng, C. S. Cheng, J. Yu, L. Meng, R. F. Yang, S. T. Lai, Z. H. Guo, Y. Xie, W. F. Chen, and H. H. Yang. 2004. T-cell epitopes in severe acute respiratory syndrome (SARS) coronavirus spike protein elicit a specific T-cell immune response in patients who recover from SARS. J. Virol. 78:5612-5618.
27. Woo, P. C., S. K. Lau, H. W. Tsol, K. H. Chan, B. H. Wong, X. Y. Che, V. K. Tam, S. C. Tam, V. C. Cheng, I. F. Hung, S. S. Wong, B. J. Zheng, Y. Guan, and K. Y. Yuen. 2004. Relative rates of non-pneumonic SARS coronavirus infection and SARS coronavirus pneumonia. Lancet 363:841-845.
28. Woo, P. C., S. K. Lau, B. H. Wong, K. H. Chan, W. T. Hui, G. S. Kwan, J. S. Peiris, R. B. Couch, and K. Y. Yuen. 2004. False-positive results in a recombinant severe acute respiratory syndrome-associated coronavirus (SARS-CoV) nucleocapsid enzyme-linked immunosorbent assay due to HCoV-OC43 and HCoV-229E rectified by Western blotting with recombinant SARS-CoV spike polypeptide. J. Clin. Microbiol. 42:5885-5888.
29. Wun, H. L., D. T. M. Leung, K. C. Wong, Y. L. Chui, and P. L. Lim. 2001. Molecular mimicry: anti-DNA antibodies may arise inadvertently as a response to antibodies generated to microorganisms. Int. Immunol. 13:1099-1107.
30. Yip, C. W., C. C. Hon, F. Zeng, K. Y. Chow, and F. C. Leung. 2004. Prevalence of non-pneumonic infections with SARS-correlated virus. Lancet 363:1825-1827.
31. Yu, F., M. Q. Le, S. Inoue, H. T. Thai, F. Hasebe, M. Del Carmen Parquet, and K. Morita. 2005. Evaluation of inapparent nosocomial severe acute respiratory syndrome coronavirus infection in Vietnam by use of highly specific recombinant truncated nucleocapsid protein-based enzyme-linked immunosorbent assay. Clin. Diagn. Lab. Immunol. 12:848-854.
32. Yu, S., M. Qiu, Z. Chen, X. Ye, Y. Gao, A. Wei, X. Wang, L. Yang, J. Wang, J. Wen, Y. Song, D. Pei, E. Dai, Z. Guo, C. Cao, J. Wang, and R. Yang. 2005. Retrospective serological investigation of severe acute respiratory syndrome coronavirus antibodies in recruits from mainland China. Clin. Diagn. Lab. Immunol. 12:552-554.
33. Zakhartchouk, A. N., S. Viswanathan, J. B. Mahony, J. Gauldie, and L. A. Babiuk. 2005. Severe acute respiratory syndrome coronavirus nucleocapsid protein expressed by an adenovirus vector is phosphorylated and immunogenic in mice. J. Gen. Virol. 86:211-215.
34. Zheng, B. J., K. H. Wong, J. Zhou, K. L. Wong, B. W. Young, L. W. Lu, and S. S. Lee. 2004. SARS-related virus predating SARS outbreak, Hong Kong. Emerg. Infect. Dis. 10:176-178.