Subconductance states of Cx30 gap junction channels: Data from transfected HeLa cells versus data from a mathematical model

Biophysical Journal

Volumn 91, Issue 6, 2006, Pages 2337-2348

  Vogel, Rolf ^a   Valiunas, Virginijus ^b,c   Weingart, Robert ^b  

^a UNIVERSITY OF BERN   (Switzerland)

^b UNIVERSITY OF BERN   (Switzerland)

^c STONY BROOK UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CONNEXIN 30; MEMBRANE PROTEIN;

ARTICLE; CELL MEMBRANE CONDUCTANCE; CONTROLLED STUDY; GAP JUNCTION; GENETIC TRANSFECTION; HELA CELL; HUMAN; HUMAN CELL; MATHEMATICAL MODEL; MEMBRANE CHANNEL; PROTEIN EXPRESSION; VOLTAGE CLAMP;

EID: 33748469566     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1529/biophysj.106.084186     Document Type: Article

References (34)

1. Unger, V. M., N. M. Kumar, N. B. Gilula, and M. Yeager. 1999. Three-dimensional structure of a recombinant gap junction membrane channel. Science. 283:1176-1180.
2. Willecke, K., J. Eiberger, J. Degen, D. Eckhardt, A. Romualdi, M. Güldenagel, J. Deutsch, and G. Söhl. 2002. Structural and functional diversity of connexin genes in the mouse and human genome. J. Biol. Chem. 383:725-737.
3. Somogyi, R., and H.-A. Kolb. 1988. Cell-to-cell channel conductance during loss of gap junctional coupling in pairs of pancreatic acinar and Chinese hamster ovary cells. Pflugers Arch. 412:54-65.
4. Chen, Y. H., and R. L. De Haan. 1992. Multiple channel conductance states and voltage regulation of embryonic chick cardiac gap junctions. J. Membr. Biol. 127:95-111.
5. Perez-Armendariz, E. M., M. C. Romano, J. Luna, C. Miranda, M. V. L. Bennett, and A. P. Moreno. 1994. Characterization of gap junctions between pairs of Leydig cells from mouse testis. Am. J. Physiol. 267:C570-C580.
6. Veenstra, R. D., H. Z. Wang, E. C. Beyer, S. V. Ramanan, and P. R. Brink. 1994. Connexin37 forms high conductance gap junction channels with subconductance state activity and selective dye and ionic permeabilities. Biophys. J. 66:1915-1928.
7. Bukauskas, F. F., C. Elfgang, K. Willecke, and R. Weingart. 1995. Biophysical properties of gap junction channels formed by mouse connexin40 in induced cell pairs of transfected human HeLa cells. Biophys. J. 68:2289-2298.
8. Valiunas, V., F. F. Bukauskas, and R. Weingart. 1997. Conductances and selective permeability of connexin43 gap junction channels examined in neonatal rat heart cells. Circ. Res. 80:708-719.
9. Oh, S., J. B. Rubin, M. V. L. Bennett, V. K. Verselis, and T. A. Bargiello. 1999. Molecular determinants of electrical rectification of single channel conductance in gap junctions formed by connexins 26 and 32. J. Gen. Physiol. 114:339-364.
10. Hopperstad, M. G., M. Srinivas, and D. C. Spray. 2000. Properties of gap junction channels formed by Cx46 alone and in combination with Cx50. Biophys. J. 79:1954-1966.
11. Sakai, R., C. Elfgang, R. Vogel, K. Willecke, and R. Weingart. 2003. The electrical behaviour of rat connexin46 gap junction channels expressed in transfected HeLa cells. Pflugers Arch. 446:714-727.
12. Churchill, D., and S. Caveney. 1993. Double whole-cell patch-clamp characterization of gap junctional channels in isolated insect epidermal cell pairs. J. Membr. Biol. 135:165-180.
13. Ramanan, S. V., and P. R. Brink. 1993. Multichannel recordings from membranes which contain gap junctions. II. Substates and conductance shifts. Biophys. J. 65:1387-1395.
14. Bukauskas, F. F., and R. Weingart. 1993. Multiple conductance states of newly formed single gap junction channels between insect cells. Pflugers Arch. 423:152-154.
15. Bukauskas, F. F., and R. Weingart. 1994. Voltage-dependent gating of single gap junction channels in an insect cell line. Biophys. J. 67:613-625.
16. Trexler, E. B., M. V. L. Bennett, T. A. Bargiello, and V. K. Verselis. 1996. Voltage gating and permeation in a gap junction hemichannel. Proc. Natl. Acad. Sci. USA. 93:5836-5841.
17. Valiunas, V., and R. Weingart. 2000. Electrical properties of gap junction hemichannels identified in transfected HeLa cells. Pflugers Arch. 440:366-379.
18. Christ, G. J., and P. R. Brink. 1999. Analysis of the presence and physiological relevance of subconducting states of connexin43-derived gap junction channels in cultured human corporal vascular smooth muscle cells. Circ. Res. 84:797-803.
19. Valiunas, V., D. Manthey, R. Vogel, K. Willecke, and R. Weingart. 1999. Biophysical properties of mouse connexin30 gap junction channels studied in transfected human HeLa cells. J. Physiol. 519:631-644.
20. Vogel, R., and R. Weingart. 1997. Mathematical model of vertebrate gap junctions derived from electrical measurements on homotypic and heterotypic channels. J. Physiol. 510:177-189.
21. Vogel, R., and R. Weingart. 2002. The electrophysiology of gap junctions and gap junction channels and their mathematical modelling. Biol. Cell. 94:501-510.
22. Valiunas, V., D. Manthey, K. Willecke, and R. Weingart. 1996. Electrical properties of connexin30 gap junction channels studied in transfected HeLa cells. Pflugers Arch. 431(Suppl):R93.
23. Weingart, R., V. Valiunas, K. Willecke, and D. Manthey. 1996. Electrical properties of Cx30 gap junction channels studied in transfected HeLa cells. Keystone Conference: Molecular Aspects to the Function of Intercellular Junctions, Lake Tahoe, CA. 43 pp.
24. Dahl, E., D. Manthey, Y. Chen, H.-J. Schwartz, Y. S. Chang, P. A. Lalley, B. C. Nicholson, and K. Willecke. 1996. Molecular cloning and functional expression of mouse connexin-30, a gap junction gene highly expressed in adult brain and skin. J. Biol. Chem. 271:17903-17910.
25. Krisciukaitis, A. 1997. Computer programs for investigation of intercellular communication using double whole-cell voltage clamp. Electron. Elect. Eng. (Lithuania) 4:71-83.
26. Schmilinsky-Fluri, G., V. Valiunas, M. Willi, and R. Weingart. 1997. Modulation of cardiac gap junctions: The mode of action of arachidonic acid. J. Mol. Cell. Cardiol. 29:1703-1713.
27. Kullmann, D. M. 1989. Application of the expectation-maximization algorithm to quantal analysis of postsynaptic potentials. J. Neurosci. Methods. 30:231-245.
28. Harris, A. L. 2001. Emerging issues of connexin channels: biophysics fills the gap. Q. Rev. Biophys. 34:325-472.
29. Zagotta, W. N., T. Hoshi, and R. W. Aldrich. 1990. Restoration of inactivation in mutants of Shaker potassium channels by a peptide derived from ShB. Science. 250:568-571.
30. Barrio, L. C., J. Capel, J. A. Jarillo, C. Castro, and A. Revilla. 1997. Species-specific voltage-gating properties of connexin-45 junctions expressed in Xenopus oocytes. Biophys. J. 73:757-769.
31. Valiunas, V., R. Vogel, and R. Weingart. 2000. The kinetics of gap junction currents are sensitive to the ionic composition of the pipette solution. Pflugers Arch. 440:835-842.
32. Banach, K., and R. Weingart. 2000. Voltage gating of Cx43 gap junction channels involves fast and slow current transitions. Pflugers Arch. 439:248-250.
33. Bukauskas, F. F., A. B. Angele, V. K. Verselis, and M. V. L. Bennett. 2002. Coupling asymmetry of heterotypic connexin 45/connexin 43-EGFP gap junctions: properties of fast and slow gating mechanisms. Proc. Natl. Acad. Sci. USA. 99:7113-7118.
34. Qu, Y., and G. Dahl. 2002. Function of the voltage gate of gap junction channels: selective exclusion of molecules. Proc. Natl. Acad. Sci. USA. 99:697-702.