LacO-LacI interaction in affinity adsorption of plasmid DNA

Biotechnology and Bioengineering

Volumn 95, Issue 1, 2006, Pages 67-75

  Forde, Gareth M ^a,d   Ghose, Siddhartha ^a   Slater, Nigel K H ^a   Hine, Anna V ^b   Darby, Richard A J ^b   Hitchcock, Anthony G ^c  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^b ASTON UNIVERSITY   (United Kingdom)

^c Stephenson Building   (United Kingdom)

^d MONASH UNIVERSITY   (Australia)

Author keywords

Affinity chromatography; Affinity ligands; LacO lacI binding; Plasmid DNA; Protein DNA interaction

Indexed keywords

AFFINITY CHROMATOGRAPHY; AMINO ACIDS; BACTERIA; BINDING ENERGY; CYTOLOGY; DENSITOMETERS; PURIFICATION;

AFFINITY LIGANDS; DENSITOMETRIC ANALYSIS; LACO/LACI BINDING; PLASMID DNA; PROTEIN/DNA INTERACTION;

DNA;

DNA FRAGMENT; DOUBLE STRANDED DNA; PLASMID DNA; PROTEIN LACL; REPRESSOR PROTEIN; UNCLASSIFIED DRUG;

ADSORPTION; AFFINITY CHROMATOGRAPHY; ARTICLE; BINDING SITE; CONTROLLED STUDY; DENSITOMETRY; DNA PURIFICATION; DNA SEQUENCE; DNA SUPERCOILING; GEL ELECTROPHORESIS; LACTOSE OPERON; NONHUMAN; POLYMERASE CHAIN REACTION; PROTEIN DNA BINDING; PROTEIN INTERACTION; SURFACE PLASMON RESONANCE;

BACTERIAL PROTEINS; BINDING SITES; CHROMATOGRAPHY, AFFINITY; DNA, BACTERIAL; DNA-BINDING PROTEINS; LAC OPERON; PLASMIDS; PROTEIN BINDING; REPRESSOR PROTEINS;

BACTERIA (MICROORGANISMS);

EID: 33748433378     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.20955     Document Type: Article

References (35)

1. Bergan D, Galbraith T, Sloane DL. 2000. Gene transfer in vitro and in vivo by cationic lipids is not significantly affected by levels of supercoiling of a reporter plasmid. Pharm Res 17(8):967-973.
2. Boles TC, White JH, Cozzarelli NR. 1990. Structure of plectonemically supercoiled DNA. J Mol Biol 213(4):931-951.
3. Chase HA. 1984. Prediction of the performance of preparative affinity chromatography. J Chromatogr 297:179-202.
4. Costioli MD, Fisch I, Garret-Flaudy F, Hilbrig F, Freitag R. 2003. DNA purification by triple-helix affinity precipitation. Biotechnol Bioeng 81(5):535-545.
5. Crystal RG. 1995. The gene as the drug. Nat Med 1(1):15-17.
6. Darby RAJ, Hine AV. 2005. LacI-mediated sequence-specific affinity purification of plasmid DNA for therapeutic applications. FASEB J 19:801-803.
7. Diogo MM, Queiroz JA, Monteiro GA, Martins SA, Ferreira GN, Prazeres DM. 2000. Purification of a cystic fibrosis plasmid vector for gene therapy using hydrophobic interaction chromatography. Biotechnol Bioeng 68(5):576-583.
8. Diogo MM, Ribeiro SC, Queiroz JA, Monteiro GA, Tordo N, Perrin P, Prazeres DM. 2001. Production, purification and analysis of an experimental DNA vaccine against rabies. J Gene Med 3(6):577-584.
9. Ferreira GNM, Monteiro GA, Prazeres DMF, Cabral JMS. 2000. Downstream processing of plasmid DNA for gene therapy and DNA vaccine applications. Trends Biotechnol 18(9):380-388.
10. Food and Drug Administration. 1996. Points to consider on plasmid DNA vaccines for preventive infectious disease indications. Center for Biologics Evaluation and Research, Office of Vaccine Research and Review. US Docket No. 96n-0400. http://www.fda.gov/cber/gdlns/plasmid.pdf.
11. Fox JL. 1999. US food labeling policy softens. Nat Biotechnol 17(9):847.
12. Fried M, Crothers DM. 1981. Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis. Nucleic Acids Res 9(23):6505-6525.
13. Fried MG, Stickle DF, Smirnakis KV, Adams C, MacDonald D, Lu P. 2002. Role of hydration in the binding of lac repressor to DNA. J Biol Chem 277(52):50676-50682.
14. Ghose S, Forde GM, Slater NK. 2004. Affinity adsorption of plasmid DNA. Biotechnol Prog 20(3):841-850.
15. Green AP, Prior GM, Helveston NM, Taittinger BE, Liu X, Thompson JA. 1997. Preparative purification of supercoiled plasmid DNA for therapeutic applications. BioPharm 52-61.
16. Ito T, Smith CL, Cantor CR. 1992. Sequence-specific DNA purification by triplex affinity capture. Proc Natl Acad Sci USA 89(2):495-498.
17. Luo D, Saltzman WM. 2000. Synthetic DNA delivery systems. Nat Biotechnol 18(1):33-37.
18. Lyddiatt A, O'Sullivan DA. 1998. Biochemical recovery and purification of gene therapy vectors. Curr Opin Biotechnol 9(2):177-185.
19. Mallinckrodt Baker Inc. 2003a. Potassium chloride materials safety data sheet. Mallinckrodt Chemicals Environments Health and Safety, USA. Phillipsburg, NJ.
20. Mallinckrodt Baker Inc. 2003b. Sodium chloride materials safety data sheet. Mallinckrodt Chemical Environments Health and Safety, USA. Phillipsburg, NJ.
21. Marquet M, Horn NA, Meek JA. 1995. Process development for the manufacture of plasmid DNA vectors for use in gene therapy. BioPharm 8:26-27.
22. Mountain A. 2000. Gene therapy: The first decade. Trends Biotechnol 18(3):119-128.
23. Muller J, Oehler S, Muller-Hill B. 1996. Repression of lac promoter as a function of distance, phase and quality of an auxiliary lac operator. J Mol Biol 257(1):21-29.
24. Oehler S, Eismann ER, Kramer H, Muller-Hill B. 1990. The three operators of the lac operon cooperate in repression. Embo J 9(4):973-979.
25. Opolski MP. 2000. Multicomponent complex for use with substrate. United States Patent 6096726. Surface Solutions Laboratories Incorporated, Carlisle, MA.
26. Pfahl M. 1981. Characteristics of tight binding repressors of the lac operon. J Mol Biol 147(1):1-10.
27. Sadler JR, Sasmor H, Betz JL. 1983. A perfectly symmetric lac operator binds the lac repressor very tightly. Proc Natl Acad Sci USA 80(22): 6785-6789.
28. Schluep T, Cooney CL. 1998. Purification of plasmids by triplex affinity interaction. Nucleic Acids Res 26(19):4524-4528.
29. Sofer G, Hagel L. 1997. Handbook of process chromatography: A guide to optimisation, Scale-up and validation. San Diego, USA: Academic Press.
30. Stadler J, Lemmens R, Nyhammar T. 2004. Plasmid DNA purification. J Gene Med 6 (Suppl 1):S54-S66.
31. Stanley SB, Crothers DM. 1987. Lac repressor is a transient gene-activating protein. Cell 51:699-707.
32. Varley DL, Hitchcock AG, Weiss AM, Horler WA, Cowell R, Peddie L, Sharpe GS, Thatcher DR, Hanak JA. 1999. Production of plasmid DNA for human gene therapy using modified alkaline cell lysis and expanded bed anion exchange chromatography. Bioseparation 8(1-5): 209-217.
33. Wils P, Escriou V, Warnery A, Lacroix F, Lagneaux D, Ollivier M, Crouzet J, Mayaux JF, Scherman D. 1997. Efficient purification of plasmid DNA for gene transfer using triple-helix affinity chromatography. Gene Ther 4(4):323-330.
34. Winter RB, von Hippel PH. 1981. Diffusion-driven mechanisms of protein translocation on nucleic acids 2. The Escherichia coli repressor-operator interaction: equilibrium measurements. Biochemistry 20(24): 6948-6960.
35. Woodgate J, Palfrey D, Nagel DA, Hine AV, Slater NKH. 2002. Protein-mediated isolation of plasmid DNA by a zinc finger-glutathione S-transferase affinity linker. Biotechnol Bioeng 79:450-456.