Phosphodiester modification by zinc metalated adenine polymer with carboxyl pendants

Bioorganic and Medicinal Chemistry Letters

Volumn 16, Issue 20, 2006, Pages 5364-5367

  Gupta, Yogita ^a   Mathur, G N ^a   Parvez, Masood ^b   Verma, Sandeep ^a  

^a INDIAN INSTITUTE OF TECHNOLOGY KANPUR   (India)

^b UNIVERSITY OF CALGARY   (Canada)

Author keywords

Adenine; Catalysis; Plasmid cleavage; Polymer; Zinc

Indexed keywords

ADENINE DERIVATIVE; BIS(4 NITROPHENYL) PHOSPHATE; CARBOXYL GROUP; ESTER DERIVATIVE; PHOSPHODIESTER DERIVATIVE; POLYMER; UNCLASSIFIED DRUG; ZINC;

ARTICLE; CATALYSIS; CHEMICAL MODIFICATION; CHEMICAL REACTION; DNA SUPERCOILING; HYDROLYSIS; METALLATION; PH; PLASMID; REACTION ANALYSIS;

ADENINE; CATALYSIS; DIMERIZATION; DNA, SUPERHELICAL; ESTERS; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; KINETICS; MODELS, MOLECULAR; POLYMERS; TIME FACTORS; ZINC;

KOBUS;

EID: 33748424139     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2006.07.077     Document Type: Article

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30. pBR322 cleavage experiment. All cleavage reactions were performed by incubating 100 μg AP1-Zn (corresponding to 0.127 mM of zinc concentration, if the polymer was to be completely soluble in buffer) in sodium cacodylate buffer (10 mM, pH 7.5), at 35 °C, unless otherwise mentioned. Total reaction volume was 20 μL and weight of the DNA was 8 ng/μL of buffer. Two microliters of methanol was used for polymer wetting. All reactions were quenched with 5 μL of loading buffer containing 100 mM EDTA, 50% glycerol in Tris-HCl, pH 8.0, at regular time intervals. The samples were loaded onto 0.7% of agarose gel containing ethidium bromide (1 μg/mL) and were electrophoresed for 1 h at constant current, 70 mA. Finally gels were imaged on PC-interfaced Bio-Rad Gel Documentation system 2000.