Enhanced DNA transfer into fish bone cells using polyethylenimine

Molecular Biotechnology

Volumn 34, Issue 1, 2006, Pages 51-54

  Braga, Daniel ^a   Laizé, Vincent ^a   Tiago, Daniel M ^a   Cancela, M Leonor ^a  

^a UNIVERSITY OF ALGARVE   (Portugal)

Author keywords

Cell transfection; DNA transfer; Non mammalian cells; Polyethylenimine (PEI)

Indexed keywords

BONE; CELL CULTURE; COST EFFECTIVENESS; DNA; OPTIMIZATION;

CELL TRANSFECTION; NON-MAMMALIAN CELLS; POLYETHYLENIMINE (PEI); TARGET CELLS;

GENETIC ENGINEERING;

DNA; ENHANCED GREEN FLUORESCENT PROTEIN; LUCIFERASE; POLYETHYLENEIMINE;

ANIMAL CELL; ARTICLE; BONE CELL; DNA TRANSFER; DNA VECTOR; FISH; INTERMETHOD COMPARISON; NONHUMAN;

ANIMALS; CELL CULTURE TECHNIQUES; CELLS, CULTURED; COATED MATERIALS, BIOCOMPATIBLE; DNA; DRUG CARRIERS; MATERIALS TESTING; OSTEOBLASTS; POLYETHYLENEIMINE; SEA BREAM; TRANSFECTION;

MAMMALIA;

EID: 33748370298     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1385/MB:34:1:51     Document Type: Article

References (9)

1. Du, S. L. and Haga, Y. (2004) The Zebrafish as a model for studying skeletal development, in Biomineralization: Progress in Biology, Molecular Bbiology and Application (Baeuerlein, E., ed.), Willey-VCH, Weinheim, Germany, pp. 283-304.
2. Inohaya, K. and Kudo, A. (2000) Medaka as a model organism of skeletal development. Tanpakushitsu Kakusan Koso 45, 2745-2751.
3. Pombinho, A. R., Laizé, V., Molha, D. M., Marques, S. M. P., and Cancela, M.L. (2004) Development of two bone-derived cell lines from the marine teleost Sparus aurata; evidence for extracellular matrix mineralization and cell-type-specific expression of matrix Gla protein and osteocalcin. Cell Tissue Res. 315, 393-406.
4. Clamme, J. P., Azoulay, J., and Mely, Y. (2003) Monitoring the formation and dissociation of Polyethylenimine/DNA complexes by two proton fluorescence correlation spectroscopy. Biophys. J. 84, 1960-1968.
5. Kunath, K., Harpe, A., Fisher, D., et al. (2003) Low-molecular-weight polyethylenimine as a non-viral vector for DNA delivery: Comparison of physicochemical properties, transfection efficiency and in vivo distribution with high-molecular-weight polyethylenimine. J. Control. Release 89, 113-125.
6. Allo, J. C., Midoux, P., Merten, M., et al. (2000) Efficient gene transfer into human normal and cystic fibrosis tracheal gland serous cells with synthetic vectors. Am. J. Respir. Cell Mol. Biol. 22, 166-175.
7. Suh, J., Wirtz, D., and Hanes, J. (2003) Efficient active transport of gene nanocarriers to the cell nucleus. Proc. Natl. Acad. Sci. U.S.A. 100, 3878-3882.
8. Florea, B. I., Meaney, C., Junginger, H. E., and Borchard, G. (2002) Transfection efficiency and toxicity of polyethylenimine in differentiated Calu-3 and non-differentiated COS-1 cell cultures. AAPS PharmSci. 4, E12.
9. Boussif, O., Lezoualc'h, F., Zanta, M. A., et al. (1995) A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: Polyethylenimine. Proc. Natl. Acad. Sci. U.S.A. 92, 7297-7301.