Cloning and characterization of genes encoding α and β subunits of glutamate-gated chloride channel protein in Cylicocyclus nassatus

Molecular and Biochemical Parasitology

Volumn 150, Issue 1, 2006, Pages 46-55

  Tandon, Ritesh ^a   LePage, Keith T ^a   Kaplan, Ray M ^a  

^a UNIVERSITY OF GEORGIA   (United States)

Author keywords

3H Ivermectin; Cyathostomin; Cylicocyclus nassatus; Glutamate gated chloride channel; Ivermectin receptor

Indexed keywords

AVERMECTIN; CHLORIDE CHANNEL; COMPLEMENTARY DNA; GLUTAMIC ACID; IVERMECTIN; MILBEMYCIN; TRITIUM;

ALPHA CHAIN; ANIMAL CELL; ANTIBIOTIC RESISTANCE; ARTICLE; BETA CHAIN; CAENORHABDITIS ELEGANS; CELL STRAIN COS7; CONTROLLED STUDY; COOPERIA ONCOPHORA; CYLICOCYCLUS NASSATUS; DNA LIBRARY; EXPRESSION VECTOR; GENETIC TRANSFECTION; HAEMONCHUS CONTORTUS; IC 50; LIGAND BINDING; LIQUID SCINTILLATION COUNTING; MOLECULAR CLONING; MUTATION; NEMATODE; NONHUMAN; NUCLEOTIDE SEQUENCE; ORTHOLOGY; PHYLOGENY; PRIORITY JOURNAL; SEQUENCE ANALYSIS;

AMINO ACID SEQUENCE; ANIMALS; CERCOPITHECUS AETHIOPS; CHLORIDE CHANNELS; CLONING, MOLECULAR; COS CELLS; DNA, COMPLEMENTARY; HELMINTH PROTEINS; HORSES; ION CHANNEL GATING; IVERMECTIN; MOLECULAR SEQUENCE DATA; PHYLOGENY; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; STRONGYLE INFECTIONS, EQUINE; STRONGYLOIDEA; TRANSFECTION;

CAENORHABDITIS ELEGANS; COOPERIA ONCOPHORA; CYLICOCYCLUS NASSATUS; EQUIDAE; HAEMONCHUS CONTORTUS; INVERTEBRATA; MAMMALIA; NEMATODA;

EID: 33748301926     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.molbiopara.2006.06.007     Document Type: Article

References (35)

1. Murphy D., and Love S. The pathogenic effects of experimental cyathostome infections in ponies. Vet Parasitol 70 (1997) 99-110
2. Love S., Murphy D., and Mellor D. Pathogenicity of cyathostome infection. Vet Parasitol 85 (1999) 113-121
3. Kaplan R.M. Anthelmintic resistance in nematodes of horses. Vet Res 33 (2002) 491-507
4. Kaplan R.M. Drug resistance in nematodes of veterinary importance: a status report. Trends Parasitol 20 (2004) 477-481
5. Kaplan R.M., Klei T.R., Lyons E.T., et al. Prevalence of anthelmintic resistant cyathostomes on horse farms. J Am Vet Med Assoc 225 (2004) 903-910
6. Chandrawathani P., Waller P.J., Adnan M., and Hoglund J. Evolution of high-level, multiple anthelmintic resistance on a sheep farm in Malaysia. Trop Anim Health Prod 35 (2003) 17-25
7. Mejia M.E., Igartua B.M.F., Schmidt E.E., and Cabaret J. Multispecies and multiple anthelmintic resistance on cattle nematodes in a farm in Argentina: the beginning of high resistance?. Vet Res 34 (2003) 461-467
8. West D.M., Pomroy W.E., and Leathwick D.M. Multiple resistance in Trichostrongylus and Teladorsagia (Ostertagia) in goats to oxfendazole, levamisole and moxidectin, and inefficacy of trichlorphon. N Z Vet J 52 (2004) 298-299
9. Mortensen L.L., Williamson L.H., Terrill T.H., Kircher R., Larsen M., and Kaplan R.M. Evaluation of prevalence and clinical implications of anthelmintic resistance in gastrointestinal nematodes of goats. J Am Vet Med Assoc 23 (2003) 495-500
10. Tandon R., Lyons E.T., Tolliver S.C., and Kaplan R.M. Effect of moxidectin selection on the genetic variation within Cylicocyclus nassatus based on amplified fragment length polymorphism (AFLP). Int J Parasitol 35 (2005) 813-819
11. Arena J.P., Liu K.K., Paress P.S., et al. The mechanism of action of avermectins in Caenorhabditis elegans: correlation between activation of glutamate-sensitive chloride current, membrane binding, and biological activity. J Parasitol 81 (1995) 286-294
12. Cully D.F., Vassilatis D.K., Liu K.K., et al. Cloning of an avermectin-sensitive glutamate-gated chloride channel from Caenorhabditis elegans. Nature 371 (1994) 707-711
13. Dent J.A., Davis M.W., and Avery L. avr-15 encodes a chloride channel subunit that mediates inhibitory glutamatergic neurotransmission and ivermectin sensitivity in Caenorhabditis elegans. EMBO J 16 (1997) 5867-5879
14. Laughton D.L., Lunt G.G., and Wolstenholme A.J. Reporter gene constructs suggest that the Caenorhabditis elegans avermectin receptor beta-subunit is expressed solely in the pharynx. J Exp Biol 200 (1997) 1509-1514
15. Vassilatis D.K., Elliston K.O., Paress P.S., et al. Evolutionary relationship of the ligand-gated ion channels and the avermectin-sensitive, glutamate-gated chloride channels. J Mol Evol 44 (1997) 501-508
16. Yates D.M., Portillo V., and Wolstenholme A.J. The avermectin receptors of Haemonchus contortus and Caenorhabditis elegans. Int J Parasitol 33 (2003) 1183-1193
17. Cheeseman C.L., Delany N.S., Woods D.J., and Wolstenholme A.J. High-affinity ivermectin binding to recombinant subunits of the Haemonchus contortus glutamate-gated chloride channel. Mol Biochem Parasitol 114 (2001) 161-168
18. Delany N.S., Laughton D.L., and Wolstenholme A.J. Cloning and localisation of an avermectin receptor-related subunit from Haemonchus contortus. Mol Biochem Parasitol 97 (1998) 177-187
19. Forrester S.G., Hamdan F.F., Prichard R.K., and Beech R.N. Cloning, sequencing, and developmental expression levels of a novel glutamate-gated chloride channel homologue in the parasitic nematode Haemonchus contortus. Biochem Biophys Res Commun 254 (1999) 529-534
20. Jagannathan S., Laughton D.L., Critten C.L., Skinner T.M., Horoszok L., and Wolstenholme A.J. Ligand-gated chloride channel subunits encoded by the Haemonchus contortus and Ascaris suum orthologues of the Caenorhabditis elegans gbr-2 (avr-14) gene. Mol Biochem Parasitol 103 (1999) 129-140
21. Forrester S.G., Prichard R.K., and Beech R.N. A glutamate-gated chloride channel subunit from Haemonchus contortus: expression in a mammalian cell line, ligand binding, and modulation of anthelmintic binding by glutamate. Biochem Pharmacol 63 (2002) 1061-1068
22. Yates D.M., and Wolstenholme A.J. An ivermectin-sensitive glutamate-gated chloride channel subunit from Dirofilaria immitis. Int J Parasitol 34 (2004) 1075-1081
23. Dent J.A., Smith M.M., Vassilatis D.K., and Avery L. The genetics of ivermectin resistance in Caenorhabditis elegans. Proc Natl Acad Sci USA 97 (2000) 2674-2679
24. Njue A.I., Hayashi J., Kinne L., Feng X.P., and Prichard R.K. Mutations in the extracellular domains of glutamate-gated chloride channel alpha 3 and beta subunits from ivermectin-resistant Cooperia oncophora affect agonist sensitivity. J Neurochem 89 (2004) 1137-1147
25. Lyons E., Tolliver S., Drudge J., Stamper S., Swerczek T., and Granstrom D. A study of population dynamics of endoparasites featuring benzimidazole-resistant small strongyles (population S) in Shetland ponies. Vet Parasitol 66 (1996) 75-86
26. Lichtenfels J.R., Kharchenko V.A., Krecek R.C., and Gibbons L.M. An annotated checklist by genus and species of 93 species level names for 51 recognized species of small strongyles (Nematoda: Strongyloidea: Cyathostominea) of horses, asses and zebras of the world. Vet Parasitol 79 (1998) 65-79
27. Tolliver S.C. A practical method of identification of the North American cyathostomes (small strongyles) in equids in Kentucky (2000), Kentucky Experiment Station, College of Agriculture, Department of Veterinary Science, University of Kentucky
28. Leid M., Kastner P., Lyons R., et al. Purification, cloning, and Rxr Identity of the hela-cell factor with which Rar or Tr heterodimerizes to bind target sequences efficiently. Cell 68 (1992) 377-395
29. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72 (1976) 248-254
30. Horoszok L., Raymond V., Sattelle D.B., and Wolstenholme A.J. GLC-3: a novel fipronil and BIDN-sensitive, but picrotoxinin-insensitive, l-glutamate-gated chloride channel subunit from Caenorhabditis elegans. Br J Pharmacol 132 (2001) 1247-1254
31. Paiement J.P., Leger C., Ribeiro P., and Prichard R.K. Haemonchus contortus: effects of glutamate, ivermectin, and moxidectin on inulin uptake activity in unselected and ivermectin-selected adults. Exp Parasitol 92 (1999) 193-198
32. Kane N.S., Hirschberg B., Qian S., et al. Drug-resistant Drosophila indicate glutamate-gated chloride channels are targets for the antiparasitics nodulisporic acid and ivermectin. Proc Natl Acad Sci USA 97 (2000) 13949-13954
33. Van Wyk J.A., Malan F.S., Gerber H., and Alves R. Two field strains of Haemonchus contortus resistant to rafoxanide. Onderstepoort J Vet Res 54 (1987) 143-146
34. Trawford A, Burden F, Hodgkinson JE. Suspected moxidectin resistance in cyathostomes in two donkey herds at the Donkey Sanctuary, UK. Proceedings of the 20th International Conference of the World Association for the Advancement of Veterinary Parasitology, Christchurch, New Zealand, October 16-20, 2005.
35. Forrester S.G., Beech R.N., and Prichard R.K. Agonist enhancement of macrocyclic lactone activity at a glutamate-gated chloride channel subunit from Haemonchus contortus. Biochem Pharmacol 67 (2004) 1019-1024