Preliminary characterization of a thermostable DNA polymerase I from a mesophilic Bacillus sphaericus strain C3-41

Archives of Microbiology

Volumn 186, Issue 3, 2006, Pages 203-209

  Bei, Han ^a   Haizhou, Liu ^a   Xiaomin, Hu ^a   Zhiming, Yuan ^a  

^a WUHAN INSTITUTE OF VIROLOGY   (China)

Author keywords

Bacillus sphaericus; Mesophile; Mosquitocidal activity; Thermostable DNA polymerase I

Indexed keywords

BACTERIAL ENZYME; DNA DIRECTED DNA POLYMERASE BETA; HYBRID PROTEIN;

ARTICLE; BACILLUS SPHAERICUS; BACILLUS SPHAERICUS C3 41; BACTERIAL GENE; BACTERIAL STRAIN; BIOINFORMATICS; COMPARATIVE STUDY; DNA DETERMINATION; ENTOMOPATHOGENY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME STABILITY; ESCHERICHIA COLI; FLUORESCENCE; GENE OVEREXPRESSION; GENE SEQUENCE; HIGH TEMPERATURE; HOUSEKEEPING GENE; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; POLI GENE; PRIORITY JOURNAL; PROTEIN STRUCTURE; QUANTITATIVE ANALYSIS; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; STRUCTURAL GENE; STRUCTURAL PROTEOMICS; THERMOPHILIC BACTERIUM; THERMOSTABILITY;

BACILLUS; CLONING, MOLECULAR; DNA POLYMERASE I; DNA, BACTERIAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME STABILITY; ESCHERICHIA COLI; MOLECULAR SEQUENCE DATA; PHYLOGENY; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, DNA;

BACILLUS SPHAERICUS; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 33748299069     PISSN: 03028933     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00203-006-0135-3     Document Type: Article

References (27)

1. Aliotta JM, Pelletier JJ, Ware JL, Moran LS, Benner JS, Kong HM (1996) Thermostable Bst DNA polymerase I lakes a 3′ → 5′ proofreading exonuclease activity. Genet Anal 12:185-195
2. Bao K, Cohen SN (2004) Reverse transcriptase activity innate to DNA polymerase I and DNA topoisomerase I proteins of streptomyces telomere complex. Proc Natl Acad Sci 101:14361-14366
3. Bourgouin C, Delecluse A, Torre F, Szulmajster J (1990) Transfer of the toxin protein genes of Bacillus sphaericus into Bacillus thuringiensis subsp. israelensis and their expression. Appl Environ Microbiol 56:340-344
4. Bruck I, Yuzhakov A, Yurieva O, Jeruzalmi D, Skangalis M, Kuriyan J, O'Donnell M (2002) Analysis of a multicomponent thermostable DNA polymerase III replicase from an extreme thermophile. J Biol Chem 19:17334-17348
5. Cano RJ, Monica K, Borucki (1995) Revival and identification of bacterial spores in 20-to 40-million-year-old Dominican amber. Science 268:1060-1064
6. Chatelier EL, Bécherel OJ, Alencon E, Canceill D, Ehrlich SD, Fuchs RP, Janniére L (2004) Involvement of DnaE, the second replicative DNA polymerase from Bacillus subtilis, in DNA mutagenesis. J Biol Chem 16:1757-1767
7. Chien A, Edgar DB, Trela JM (1976) Deoxyribonucleic acid polymerase from the extreme thermophile Thermus aquaticus. J Bacteriol 127:1550-1557
8. Deckert G, Warren PV, Gaasterland T, Young WG, Lenox AL, Graham DE, Overbeek R, Snead MA, Keller M, Aujay M, Huber R, Feldman RA, Short JM, Olsen GJ, Swanson RV (1998) The complete genome of the hyperthermophilic bacterium Aquifex aeolicus. Nature 392:353-358
9. Harini S, Griffiths K, Flynn EK, Astatke M, Shih PJ, Lee JE, Gerard GF, Gibbs MD, Bergquist PL (2004) Thermophilic bacterial DNA polymerases with reverse-transcriptase activity. Extremophiles 8:243-251
10. Joyce CM, Steitz TA (1994) Function and structure relationships in DNA polymerases. Annu Rev Biochem 63:777-822
11. Kong HM, Pelletier JJ, Aliotta JM (1998) Over-expression and purification of truncated thermostable DNA polymerase by protein fusion. US Patent 5814506
12. Krell T, Greco1 F, Engel1 O, Dubayle J, Dubayle J, Kennel A, Charloteaux B, Brasseur R, Chevalier M, Sodoyer R, Habit RE (2004) HIV-1 gp41 and gp160 are hyperthermostable proteins in a mesophilic environment characterization of gp41 mutants. Eur J Biochem 271:1566-1579
13. Nakamura LK (2000) Phylogeny of Bacillus sphaericus-like organisms. Int J Syst Evol Microbiol 50:1715-1722
14. Pavlov AR, Pavlova N, Kozyavkin SA, Slesarev AI (2004) Recent developments in the optimization of thermostable DNA polymerases for efficient applications. Trends Biotechnol 22:253-260
15. Perler F, Kumar S, Kong H (1996) Thermostable DNA polymerase. Adv Protein Chem 48:377-435
16. Sambrook J, Russell J (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
17. Sellmann E, Schroder KL, Knoblich IM, Westermann P (1992) Purification and characterization of DNA polymerases from Bacillus species. J Bacteriol 174:4350-4355
18. Tsujimoto Y, Watanabe A, Nakano K, Watanabe K, Matsui H, Tsuji K, Tsukihara T, Suzuki Y (2003) Gene cloning, expression, and crystallization of a thermostable exo-inulinase from Geobacillus stearothermophilus KP1289. Appl Microbiol Biotechnol 62:180-185
19. Vellore J, Moretz SE, Lampson BC (2004) A group II intron-type open reading frame from the thermophile Bacillus (Geobacillus) stearothermophilus encodes a heat-stable reverse transcriptase. Appl Environ Microbiol 70:7140-7147
20. Villbrandt B, Sobek H, Frey B, Schomburg D (2000) Domain exchange: chimerase of Thermus aquaticus DNA polymerase, Escherichia coli DNA polymerase I and Thermotoga neapolitana DNA polymerase. Protein Eng 13:645-654
21. Vogt G, Woell S, Argos P (1997) Protein thermal stability, hydrogen bonds, and ion pairs. J Mol Biol 269:631-643
22. Wingren C, Edmundson AB, Borrebaeck CAK (2003) Designing proteins to crystallize through-strand pairing. Protein Eng 16:255-264
23. Xu D, Cote JC (2003) Phylogenetic relationships between Bacillus species and related genera inferred from comparison of 39 end 16S rDNA and 59 end 16S-23S ITS nucleotide sequences. Int J Syst Evol Microbiol 53:695-704
24. Yoon JH, Lee KC, Weiss N, Kho YH, Kang KH, Park YH (2001) Sporosarcina aquimarina sp. nov., a bacterium isolated from seawater in Korea, and transferof Bacillus globisporus (Larkin and Stokes 1967), Bacillus psychrophilus (Nakamura 1984) and Bacillus pasteurii (Chester 1898) to the genus Sporosarcina as Sporosarcina globispora comb. nov., Sporosarcina psychrophila comb. nov. and Sporosarcina pasteurii comb. nov., and emended description of the genus Sporosarcina. Int J Syst Evol Microbiol 51:1079-1086
25. Yuan ZM, Neilsen-LeRoux C, Pasteur N, Delecluse A, Charles JF, Frutos R (1999) Cloning and expression of the bin genes of Bacillus sphaericus C3-41 in a crystal minus B. thuringiensis subsp. Israelensis. Wei Sheng Wu Xue Bao 39:29-35
26. Yuan ZM, Rang C, Maroun RC, Victor JP, Frutos R, Pasteur N, Vendrely C, Jean-Francois C, Nielsen-LeRoux C (2001) Identification and molecular structural prediction analysis of a toxicity determinant in the Bacillus sphaericus crystal larvicidal toxin. Eur J Biochem 268:2751-2760
27. Zhang YM, Liu EY, Cai QX, Chen ZS (1987) Isolation of two high toxin bacillus sphaericus strains. Inseticidal Microorg 1:98-99