Elevated expression of human alpha-1 antitrypsin mediated by yeast intron in Pichia pastoris

Biotechnology Letters

Volumn 28, Issue 19, 2006, Pages 1545-1550

  Hasannia, S ^a   Lotfi, A S ^a   Mahboudi, F ^b   Rezaii, A ^a   Rahbarizadeh, F ^a   Mohsenifar, A ^a  

^a TARBIAT MODARES UNIVERSITY   (Iran)

^b PASTEUR INSTITUTE OF IRAN   (Iran)

Author keywords

Alpha 1 antitrypsin; Elevated gene expression; Intron mediated enhancement; Pichia pastoris introns; Trypsin inhibitory capacity

Indexed keywords

ALPHA-1 ANTITRYPSIN; ELEVATED GENE EXPRESSION; INTRON-MEDIATED ENHANCEMENT; PICHIA PASTORIS INTRONS; TRYPSIN INHIBITORY CAPACITY;

BIOASSAY; DNA; ELECTROPHORESIS; GENES; SURFACE ACTIVE AGENTS; YEAST;

GENETIC ENGINEERING;

ALPHA 1 ANTITRYPSIN; RECOMBINANT PROTEIN; TRYPSIN INHIBITOR;

ARTICLE; BIOSYNTHESIS; CELL LINE; GENE TRANSFER; GENE VECTOR; GENETICS; HUMAN; IMMUNOBLOTTING; INTRON; METABOLISM; PICHIA; POLYACRYLAMIDE GEL ELECTROPHORESIS; SACCHAROMYCES CEREVISIAE; TRANSGENE; UPREGULATION;

ALPHA 1-ANTITRYPSIN; CELL LINE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENE TRANSFER TECHNIQUES; GENETIC VECTORS; HUMANS; IMMUNOBLOTTING; INTRONS; PICHIA; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; TRANSGENES; TRYPSIN INHIBITORS; UP-REGULATION;

PICHIA PASTORIS;

EID: 33748108634     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-006-9121-8     Document Type: Article

References (15)

1. Archibald AL, McClenaghan M, Hornsey V, Simons JP, Clark AJ (1990) High-level expression of biologically active human alpha-1 antitrypsin in the milk of transgenic mice. Proc Natl Acad Sci 87(13):5178-5182
2. Ares M, Jr, Grate L, Pauling MH (1999) A handful of intron-containing genes produces the lion's share of yeast mRNA. RNA 5(9):1138-1139
3. Bourdon V, Harvey A, Lonsdale DM (2001) Introns and their positions affect the translational activity of mRNA in plant cells. EMBO Rep 2(5): 394-8
4. Brantly M, Laubach V (1995) Increased expression of alpha-1 antitrypsin in expression vectors through the inclusion of intron II. US Patent: 8, 5439824
5. Cereghino JL, Cregg JM (2000) Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol Rev 24(1):45-66
6. Gadek JE, Pacht ER (1996) Pathogenesis of hereditary emphysema and replacement therapy for alpha-1 antitrypsin deficiency: insight into the more common forms of emphysema. Chest 110(6):48S-250S
7. Huang J, Sutliff TD, Wu L, Nandi SK, Benge M, Terashima M, Ralston AH, Drohan W, Huang N, Rodriguez RL (2001) Expression and purification of functional human alpha-1-antitrypsin from cultured plant cells. Biotechnol Prog 17(1):126-133
8. Kang HA, Nam SW, Kwon KS, Chung BH, Yu MH (1996) High-level secretion of human alpha 1-antitrypsin from Saccharomyces cerevisiae using inulinase signal sequence. J Biotechnol 48(1-2):15-24
9. Kwon KS, Song M, Yu MH (1995) Purification and characterization of alpha 1-antitrypsin secreted by recombinant yeast Saccharomyces diastaticus. J Biotechnol 42(3):191-195
10. Lacy-Hulbert A, Thomas R, Li XP, Lilley CE, Coffin RS, Roes J (2001) Interruption of coding sequences by heterologous introns can enhance the functional expression of recombinant genes. Gene Therapy 8(8):649-653
11. Palmiter RD, Sandgren EP, Avarbock MR, Allen DD, Brinster RL (1991) Heterologous introns can enhance expression of transgenes in mice. Proc Natl Acad Sci 88:478-482
12. Rethmeier N, Seurinck J, Montagu M Van, Cornelissen M (1997) Intron-mediated enhancement of transgene expression in maize is a nuclear, gene-dependent process. Plant J 12(4):895-899
13. Rose AB (2004) The effect of intron location on intron-mediated enhancement of gene expression in Arabidopsis. Plant J 40(5):744-751
14. Rose AB, Beliakoff JA (2000) Intron-mediated enhancement of gene expression independent of unique intron sequences and splicing. Plant Physiol 122:535-542
15. Towbin H, Steahelin T, Gordon J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some application. Proc Natl Acad Sci 76:4350-4354