Interruption of coding sequences by heterologous introns can enhance the functional expression of recombinant genes

Gene Therapy

Volumn 8, Issue 8, 2001, Pages 649-653

  Lacy Hulbert, A ^a   Thomas, R ^a   Li, X P ^a   Lilley, C E ^a   Coffin, R S ^a   Roes, J ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

Cre loxP; Gene expression; Gene therapy; Green fluorescent protein; Herpes vectors; Intron

Indexed keywords

CRE RECOMBINASE; GREEN FLUORESCENT PROTEIN; MESSENGER RNA; RECOMBINANT PROTEIN; SIGNAL PEPTIDE; VIRUS VECTOR;

ANIMAL TISSUE; ARTICLE; BACTERIOPHAGE; BRAIN; CELL LINE; CELL TYPE; CONTROLLED STUDY; EXON; GENE CONTROL; GENE EXPRESSION; GENE FUNCTION; GENE INSERTION; GENE SEQUENCE; GENE THERAPY; GENETIC CODE; GENETIC TRANSDUCTION; HERPES SIMPLEX; IN VIVO STUDY; INTRON; MEDICINE; MUTAGENESIS; NONHUMAN; OPEN READING FRAME; POLYADENYLATION; PRIORITY JOURNAL; PROMOTER REGION; RAT; RECOMBINANT GENE; TRANSGENE; UNTRANSLATED REGION;

ANIMALS; BRAIN; CHO CELLS; CRICETINAE; EXONS; GENE EXPRESSION REGULATION; GENE THERAPY; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; INDICATORS AND REAGENTS; INTRONS; LUMINESCENT PROTEINS; RATS; RECOMBINANT PROTEINS; SIMPLEXVIRUS; TRANSFECTION;

ANIMALIA; DNA VIRUSES; HERPES; SIMPLEXVIRUS;

EID: 0035002059     PISSN: 09697128     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.gt.3301440     Document Type: Article

References (18)

1. Heterologous introns can enhance expression of transgenes in mice
2. Introns in the 3′-untranslated region can inhibit chimeric CAT and beta-galactosidase gene expression
3. Insertion of natural intron 6a-6b into a human cDNA-derived gene therapy vector for cystic fibrosis improves plasmid stability and permits facile RNA/DNA discrimination
4. Evidence for gene transfer and expression of factor IX in haemophilia B patients treated with an AAV vector
5. Green fluorescent protein as a marker for gene expression
6. Understanding, improving and using green fluorescent proteins
7. Site-specific DNA recombination in mammalian cells by the Cre recombinase of bacteriophage P1
8. Targeted DNA recombination in vivo using an adenovirus carrying the cre recombinase gene
9. Conditional gene targeting
10. Development and optimization of herpes simplex virus vectors for multiple long-term gene delivery to the peripheral nervous system
11. Self-inactivating lentiviral vectors with enhanced transgene expression as potential gene transfer system in Parkinson's disease
12. In vivo cooperation between introns during pre-mRNA processing
13. Rat growth hormone gene introns stimulate nucleosome alignment in vitro and in transgenic mice
14. Splice junctions follow a 205-base ladder
15. Gene silencing as a threat to the success of gene therapy
16. The intron requirement for immunoglobulin gene expression is dependent upon the promoter
17. A pause site for RNA polymerase II is associated with termination of transcription
18. Selective degradation of early-response-gene mRNAs: Functional analyses of sequence features of the AU-rich elements