Analysis of alternatively spliced isoforms of human LDL receptor mRNA

Clinica Chimica Acta

Volumn 373, Issue 1-2, 2006, Pages 151-157

  Tveten, Kristian ^a   Ranheim, Trine ^a   Berge, Knut Erik ^a   Leren, Trond P ^a   Kulseth, Mari Ann ^a  

^a OSLO UNIVERSITY HOSPITAL   (Norway)

Author keywords

Alternative splicing; Low density lipoprotein receptor (LDLR); Real time PCR; RT PCR

Indexed keywords

LOW DENSITY LIPOPROTEIN RECEPTOR; MESSENGER RNA;

ALTERNATIVE RNA SPLICING; ARTICLE; CELL LINE; EXON; HUMAN; HUMAN CELL; HUMAN TISSUE; PRIORITY JOURNAL; PROTEIN SYNTHESIS; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ALTERNATIVE SPLICING; CELL LINE; CELLS, CULTURED; HUMANS; PROTEIN ISOFORMS; RECEPTORS, LDL; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; SENSITIVITY AND SPECIFICITY;

EID: 33747874513     PISSN: 00098981     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.cca.2006.05.031     Document Type: Article

References (22)

1. Stamm S., Ben Ari S., Rafalska I., et al. Function of alternative splicing. Gene 344 (2003) 1-20
2. Nimpf J., and Schneider W.J. From cholesterol transport to signal transduction: low density lipoprotein receptor, very low density lipoprotein receptor, and apolipoprotein E receptor-2. Biochim Biophys Acta (BBA) Mol Cell Biol Lipids 1529 (2000) 287-298
3. Sun X.M., and Soutar A.K. Expression in vitro of alternatively spliced variants of the messenger RNA for human apolipoprotein E receptor-2 identified in human tissues by ribonuclease protection assays. Eur J Biochem 262 (1999) 230-239
4. Magrane J., Reina M., Pagan R., et al. Bovine aortic endothelial cells express a variant of the very low density lipoprotein receptor that lacks the O-linked sugar domain. J Lipid Res 39 (1998) 2172-2181
5. Marschang P., Brich J., Weeber E.J., et al. Normal development and fertility of knockout mice lacking the tumor suppressor gene LRP1b suggest functional compensation by LRP1. Mol Cell Biol 24 (2004) 3782-3793
6. Kim D.H., Magoori K., Inoue T.R., et al. Exon/intron organization, chromosome localization, alternative splicing, and transcription units of the human apolipoprotein E receptor 2 gene. J Biol Chem 272 (1997) 8498-8504
7. Goldstein J.L., Hobbs H.H., and Brown M.S. Familial hypercholesterolemia (1995) 1981-2030
8. Sudhof T.C., Goldstein J.L., Brown M.S., and Russell D.W. The LDL receptor gene: a mosaic of exons shared with different proteins. Science 228 (1985) 815-822
9. Yamamoto T., Davis C.G., Brown M.S., et al. The human LDL receptor: a cysteine-rich protein with multiple Alu sequences in its mRNA. Cell 39 (1984) 27-38
10. Wall F.E., Henkel R.D., Stern M.P., Jenson H.B., and Moyer M.P. An efficient method for routine Epstein-Barr virus immortalization of human B lymphocytes. In Vitro Cell Dev Biol Anim 31 (1995) 156-159
11. Halvorsen B., Ranheim T., Nenseter M.S., Huggett A.C., and Drevon C.A. Effect of a coffee lipid (cafestol) on cholesterol metabolism in human skin fibroblasts. J Lipid Res 39 (1998) 901-912
12. Sorensen S., Ranheim T., Bakken K.S., Leren T.P., and Kulseth M.A. Retention of mutant low density lipoprotein receptor in ER leads to ER stress. J Biol Chem 281 (2006) 468-476
13. Clatworthy A.E., Stockinger W., Christie R.H., et al. Expression and alternate splicing of apolipoprotein E receptor 2 in brain. Neuroscience 90 (1999) 903-911
14. Brandes C., Kahr L., Stockinger W., Hiesberger T., Schneider W.J., and Nimpf J. Alternative splicing in the ligand binding domain of mouse Apoe receptor-2 produces receptor variants binding Reelin but not Alpha 2-macroglobulin. J Biol Chem 276 (2001) 22160-22169
15. Lejeune F., and Maquat L.E. Mechanistic links between nonsense-mediated mRNA decay and pre-mRNA splicing in mammalian cells. Curr Opin Cell Biol 17 (2005) 309-315
16. Vandenbroucke I.I., Vandesompele J., Paepe A.D., and Messiaen L. Quantification of splice variants using real-time PCR. Nucleic Acids Res 29 (2001) e68
17. Shagin D.A., Lukyanov K.A., Vagner L.L., and Matz M.V. Regulation of average length of complex PCR product. Nucleic Acids Res 27 (1999) e23
18. Maquat L.E. Nonsense-mediated mRNA decay in mammals. J Cell Sci 118 (2005) 1773-1776
19. Lareau L.F., Green R.E., Bhatnagar R.S., and Brenner S.E. The evolving roles of alternative splicing. Curr Opin Struct Biol 14 (2004) 273-282
20. Sorek R., Shamir R., and Ast G. How prevalent is functional alternative splicing in the human genome?. Trends Genet 20 (2004) 68-71
21. Pospisil H., Herrmann A., Bortfeldt R.H., and Reich J.G. EASED: Extended Alternatively Spliced EST Database. Nucleic Acids Res 32 (2004) D70-D74
22. Graveley B.R. Alternative splicing: increasing diversity in the proteomic world. Trends Genet 17 (2001) 100-107