A simplified procedure for the reduction and alkylation of cysteine residues in proteins prior to proteolytic digestion and mass spectral analysis

Analytical Biochemistry

Volumn 333, Issue 1, 2004, Pages 174-181

  Hale, John E ^a   Butler, Jon P ^a   Gelfanova, Valentina ^a   You, Jin Sam ^a   Knierman, Michael D ^a  

^a ELI LILLY AND COMPANY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALKYLATION; AMINO ACIDS; DESALINATION; REAGENTS; SPECTRUM ANALYSIS;

CYSTEINE RESIDUES; LIQUID SAMPLE; MASS-SPECTRAL ANALYSIS; PROTEOLYTIC DIGESTION; PURIFIED PROTEIN; SIMPLIFIED PROCEDURE; SINGLE-STEP; TRIETHYLPHOSPHINE;

PROTEINS;

ALCOHOL DERIVATIVE; CYSTEINE; PHOSPHINE DERIVATIVE; REAGENT;

ALKYLATION; ARTICLE; AUTOMATION; CHEMICAL REACTION KINETICS; COMPLEX FORMATION; ELIMINATION REACTION; GAS; MASS SPECTROMETRY; PHYSICAL PHASE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN MODIFICATION; PROTEIN PURIFICATION; QUANTITATIVE ANALYSIS; REDUCTION KINETICS;

EID: 4444313548     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ab.2004.04.013     Document Type: Article

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