Conformational stability of 17β-hydroxysteroid dehydrogenase from the fungus Cochliobolus lunatus

FEBS Journal

Volumn 273, Issue 17, 2006, Pages 3927-3937

  Ulrih, Nataša Poklar ^a   Lanišnik Rižner, Tea ^a  

^a UNIVERSITY OF LJUBLJANA   (Slovenia)

Author keywords

17 hydroxysteroid dehydrogenase; Coenzyme NADPH binding; Guanidine hydrochloride; pH stability; Urea

Indexed keywords

ACID; GUANIDINE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; SODIUM CHLORIDE; TESTOSTERONE 17BETA DEHYDROGENASE; UREA;

ALPHA HELIX; ARTICLE; BETA SHEET; CIRCULAR DICHROISM; COCHLIOBOLUS; COCHLIOBOLUS LUNATUS; ENTHALPY; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME CONFORMATION; ENZYME DENATURATION; ENZYME STABILITY; HYDROPHOBICITY; IONIC STRENGTH; NONHUMAN; PH MEASUREMENT; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRECIPITATION; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN SECONDARY STRUCTURE; STRUCTURE ACTIVITY RELATION; TEMPERATURE; THERMODYNAMICS; THERMOSTABILITY; TITRIMETRY; ULTRAVIOLET SPECTROPHOTOMETRY; X RAY DIFFRACTION;

17-HYDROXYSTEROID DEHYDROGENASES; ASCOMYCOTA; CIRCULAR DICHROISM; ENZYME STABILITY; GUANIDINE; HYDROGEN-ION CONCENTRATION; PROTEIN CONFORMATION; PROTEIN DENATURATION; RECOMBINANT PROTEINS; SPECTROPHOTOMETRY, ULTRAVIOLET; TEMPERATURE; UREA;

COCHLIOBOLUS LUNATUS; FUNGI;

EID: 33747485606     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2006.05396.x     Document Type: Article

References (36)

1. Oppermann U, Filling C, Hult M, Shafgat N, Wu X, Lindh M, Shafqat J, Nordling E, Kallberg Y, Persson B et al. (2003) Short-chain dehydrogenases/ reductases (SDR): the 2002 update. Chem Biol Interact 143/144, 247-253.
2. Kallberg Y, Oppermann U, Jornvall H & Persson B (2002) Short-chain dehydrogenase/reductase (SDR) relationships: a large family with eight clusters common to human, animal, and plant genomes. Protein Sci 11, 636-641.
3. Lanišnik Rižner T, Žakelj-Mavrič M, Plemenitaš A & Zorko M (1996) Purification and characterization of 17β-hydroxysteroid dehydrogenase from the filamentous fungus Cochliobus lunatus. J Steroid Biochem Mol Biol 59, 205-214.
4. Lanišnik Rižner T, Moeller G, Thole HH, Žakelj-Mavrič M & Adamski J (1999) A novel 17β-hydroxysteroid dehydrogenase in the fungus Cochliobus lunatus: new insights into the evolution of steroid-hormone signalling. Biochem J 337, 425-431.
5. Lanišnik Rižner T, Stojan J & Adamski J (2001) Searching for the physiological function of 17β-hydroxysteroid dehydrogenase from the fungus Cochliobolus lunatus: studies of substrate specificity and expression analysis. Mol Cell Endocrinol 171, 193-198.
6. Andersson A, Jordan D, Schneider G & Lundqvist Y (1996) Crystal structure of the ternary complex of 1,3,8-trihydroxynaphthalene reductase from Magnaporthe grisea with NADPH and an active-site inhibitor. Structure 4, 1161-1170.
7. Thompson JE, Basarab GS, Andersson A, Lundqvist Y & Jordan DB (1997) Trihydroxynaphthalene reductase from Magnaporthe grisea: realization of an active center inhibitor and elucidation of the kinetic mechanism. Biochemistry 36, 1852-1860.
8. Thompson JE, Fahnestock S, Farral L, Liao D, Valent B & Jordan DB (2000) The second naphthol reductase of fungal melanin biosynthesis in Magnaporthe grisea - tetrahydroxynaphthalene reductase. J Biol Chem 275, 34867-34872.
9. Liao D, Thompson JE, Fahnestock S, Valent B & Jordan DB (2001) A structural account of substrate and inhibitor specificity differences between two naphthol reductases. Biochemistry 40, 8696-8704.
10. Jordan DB, Basarab GS, Liao D, Johnson WMP, Winzenberg KN & Winkler DA (2001) Structure-based design of inhibitors of the rice blast fungal enzyme trihydroxynaphthalene reductase. Mol Graph Model 19, 434-447.
11. Lanišnik Rižner T, Adamski J & Stojan J (2000) 17β-Hydroxysteroid dehydrogenase from Cochliobolus lunatus: model structure and substrate specificity. Arch Biochem Biophys 384, 255-262.
12. Lanišnik Rižner T, Stojan J & Adamski J (2001) 17β-Hydroxysteroid dehydrogenase from the fungus Cochliobolus lunatus: structural and functional aspects. Chem Biol Interact 130-132, 793-803.
13. Kristan K, Stojan J, Möller G, Adamski J & Lanišnik Rižner T (2005) Coenzyme specificity in fungal 17β-hydroxysteroid dehydrogenase. Mol Cell Endocrinol 241, 80-87.
14. Kristan K, Deluca D, Adamski J, Stojan J & Lanišnik Rižner T (2005) Dimerization and enzymatic activity of fungal 17β-hydroxysteroid dehydrogenase from the short-chain dehydrogenase/ reductase superfamily. BMC Biochem 6, 28.
15. Fomitcheva J, Baker ME, Anderson E, Lee GY & Aziz N (1998) Characterization of Ke 6, a new 17 betahydroxysteroid dehydrogenase, and its expression in gonadal tissues. J Biol Chem 273, 22664-22671.
16. Li A, Tedde R, Krozowski ZS, Pala A, Li KX, Shackleton CH, Mantero F, Palermo M & Stewart PM (1998) Molecular basis for hypertension in the 'type II variant' of apparent mineralocorticoid excess. Am J Hum Genet 63, 370-379.
17. He XZ, Merz G, Mehta P, Schultz H & Yang SY (1999) Human brain short chain L-3-hydroxyacyl coenzyme A dehydrogenase is a single-domain multifunctional enzyme - characterization of a novel 17 beta-hydroxysteroid dehydrogenase. J Biol Chem 274, 15014-15019.
18. Lin SX, Han Q, Azzi A, Zhu D, Gangloff A & Campbell RL (2000) 3D structure of human estrogenic 17 beta-HSD1: binding with various steroids. J Steroid Biochem Mol Biol 69, 425-429.
19. Masuzaki H, Peterson J, Shinyama H, Morton NM, Mullins JJ, Seckl JR & Flier JS (2001) A transgenic model of visceral obesity and the metabolic syndrome. Science 294, 2071-2072.
20. Sreerama N & Woody RW (2000) Circular dichroism of peptides and proteins. In Circular Dichroism: Principles and Applications (Berova N, Nakanishi K & Woody RW, eds), pp. 601-620. Wiley, New York.
21. Provencher SW & Glöckner J (1981) Estimation of globular protein secondary structure from circular dichroism. Biochemistry 20, 33-37.
22. Cassetta A, Budefeld T, Lanišnik Rižner T, Kristan K, Stojan J & Lamba D (2005) Crystallization, X-ray diffraction analysis and phasing of 17β-hydroxysteroid dehydrogenase from the fungus Cochliobolus lunatus. Acta Crystallogr F61, 1032-1034.
23. Poklar N, Lah J, Salobir M, Maček P & Vesnaver G (1997) pH and temperature-induced molten globule-like denatured states of equinatoxin II: a study by UV-melting, DSC, far- and near-UV CD spectroscopy and ANS fluorescence. Biochemistry 36, 14345-14352.
24. Schellman JA (1978) Solvent denaturation. Biopolymers 17, 1305-1322.
25. Green RF & Pace CN (1974) Urea and guanidine hydrochloride denaturation of ribonuclease, lysozyme, α-chymotrypsin, and β-lactoglobulin. J Biol Chem 249, 5388-5393.
26. Goto Y & Fink AL (1989) Conformational states of beta-lactamase: molten-globule states at acidic and alkaline pH with high salt. Biochemistry 28, 945-952.
27. Uversky VN, Kutyshenko VP, Protasova NY, Rogov VV, Vassilenko KS & Gudkov AT (1996) Circularly permuted dihydrofolate reductase possesses all the properties of the molten globule state, but can resume functional tertiary structure by interaction with its ligand. Protein Sci 5, 1844-1851.
28. Opperman UCT, Filling C, Berndt KD, Persson B, Ladenstein R & Jörnvall H (1997) Active site directed mutagenesis of 3β/17β- hydroxysteroid dehydrogenase establishes differential effects on short-chain dehydrogenase/reductase reactions. Biochemistry 36, 34-40.
29. Mendoza-Herandez G & Rendon JL (1996) Human placental estradiol 17β-dehydrogenase: structural and catalytic changes during urea-denaturation. Biochem Biophys Acta 1297, 219-227.
30. Nakanishi M, Kakumoto M, Matsuura K, Deyashiki Y, Tanaka N, Nonaka T, Mitsui Y & Hara A (1996) Involvement of two basic residues (Lys-17 and Arg-39) of mouse lung carbonyl reductase in NADP(H) binding and fatty acid activation: site-directed mutagenesis and kinetic analyses. J Biochem 120, 257-263.
31. Tanaka N, Nonaka T, Tanabe T, Yoshimoto T, Tsuru D & Mitsui Y (1996) Crystal structures of the binary and ternary complexes of 7 alpha-hydroxysteroid dehydrogenase from Escherichia coli. Biochemistry 35, 7715-7730.
32. Pace CN, Vajdos LF, Grimsley G & Gray T (1995) How to measure and predict the molar absorption coefficient of a protein? Protein Sci 4, 2411-2423.
33. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
34. McLelain T (1982) Electrophoresis buffers for polyacrylamide gels at various pH. Anal Biochem 126, 94-99.
35. Ornstein L (1964) Disc electrophoresis I. Ann NY Acad Sci 121, 321.
36. Pharmacia LKB Biotechnology (1987) Pharmacia Phast-System Users' Manual. Pharmacia LKB Biotechnology, Uppsala, Sweden.