Efficient transformation of the osmotolerant yeast Zygosaccharomyces rouxii by electroporation

Journal of Microbiological Methods

Volumn 55, Issue 2, 2003, Pages 481-484

  Pribylova, Lenka ^a   Sychrova, Hana ^a  

^a INSTITUTE OF PHYSIOLOGY   (Czech Republic)

Author keywords

Electroporation; Osmotolerance; Transformation; Zygosaccharomyces rouxii

Indexed keywords

ARTICLE; CELL CULTURE; CELL TRANSFORMATION; CELL WALL; CULTURE MEDIUM; ELECTROCHEMICAL ANALYSIS; ELECTROPORATION; FUNGAL STRAIN; FUNGUS GROWTH; FUNGUS MUTANT; NONHUMAN; PRIORITY JOURNAL; TEMPERATURE; WILD TYPE; YEAST; YEAST CELL; ZYGOSACCHAROMYCES ROUXII;

ZYGOSACCHAROMYCES ROUXII;

EID: 0141532261     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-7012(03)00197-0     Document Type: Article

References (13)

1. Bloch J.C., Sychrova H., Souciet J.L., Jund R., Chevallier M.R. Determination of a specific region of the purine-cytosine permease involved in the recognition of its substrates. Mol. Microbiol. 6:1992;2989-2997.
2. Ito H., Fukuda Y., Murata K., Kimura A. Transformation of intact yeast cells treated with alkali cations. J. Bacteriol. 153:1983;163-168.
3. Kasuske A., Wedler H., Schulze S., Becher D. Efficient electropulse transformation of intact Candida maltosa cells by different homologous vector plasmids. Yeast. 8:1992;691-697.
4. Kurtzman C.P., Fell J.W. The Yeasts, A Taxonomic Study. 1998;Elsevier, New York.
5. Meilhoc E., Masson J.M., Teissie J. High efficiency transformation of intact yeast cells by electric field pulses. Biotechnology (NY). 8:1990;223-227.
6. Ogawa Y., Tatsumi H., Murakami S., Ishida Y., Murakami K., Masaki A., Kawabe H., Arimura H., Nakano E., Motai H. Secretion of Aspergillus oryzae alkaline protease in an osmophilic yeast, Zygosaccharomyces rouxii. Agric. Biol. Chem. 54:1990;2521-2529.
7. Passoth V.V., Hahn-Hagerdahl B. Production of a heterologous endo-1,4-betaxylanase in the yeast Pichia stiptis with an O(2)-regulated promoter. Enzyme Microb. Technol. 26:2000;781-784.
8. Suga M., Hatakeyama T. High efficiency transformation of Schizosaccharomyces pombe pretreated with thiol compounds by electroporation. Yeast. 18:2001;1015-1021.
9. Sychrova H. Molecular cloning and sequence analysis of the Zygosaccharomyces rouxii LEU2 gene encoding a beta-isopropylmalate dehydrogenase. Yeast. 18:2001;989-994.
10. Thompson J.R., Register E., Curotto J., Kurtz M., Kelly R. An improved protocol for the preparation of yeast cells for transformation by electroporation. Yeast. 14:1998;565-571.
11. Tomita M., Iwata S., Yamamoto S. Alteration in cell wall chitin of Zygosaccharomyces rouxii. J. Ferment. Bioeng. 81:1996;171-173.
12. Ushio K., Tatsumi H., Araki H., Toh-E A., Oshima Y. Construction of a host-vector system in the osmophilic haploid yeast Zygosaccharomyces rouxii. J. Ferment. Technol. 66:1988;481-488.
13. van Zyl P., Killian S.G., Prior B.A. The role of an active transport mechanism in glycerol accumulation during osmoregulation by Zygosaccharomyces rouxii. Appl. Microbiol. Biotechnol. 34:1990;231-235.