Purification of recombinant human epidermal growth factor secreted from the methylotrophic yeast Hansenula polymorpha

Protein Expression and Purification

Volumn 24, Issue 1, 2002, Pages 117-122

  Heo, Joo Hyung ^a   Won, Hye Soon ^a   Kang, Hyun Ah ^a   Rhee, Sang Ki ^a   Chung, Bong Hyun ^a  

^a Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

HANSENULA POLYMORPHA; PICHIA; PICHIA ANGUSTA; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

CARBOXYPEPTIDASE; CARBOXYPEPTIDASE D; EPIDERMAL GROWTH FACTOR; HYBRID PROTEIN;

ARTICLE; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; PICHIA; SACCHAROMYCES CEREVISIAE; SECRETION;

CARBOXYPEPTIDASES; CLONING, MOLECULAR; EPIDERMAL GROWTH FACTOR; HUMANS; PICHIA; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE;

EID: 0036065504     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2001.1527     Document Type: Article

References (20)

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13. Development of expression systems for the production of recombinant proteins in Hansenula polymorpha DL-1
14. Gene expression and secretion of human epidermal growth factor in a methylotrophic yeast Hansenula polymorpha
15. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
16. A novel autonomously replicating sequence (ARS) for multiple integration in the yeast Hansenula polymorpha DL-1
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