Applications of LightCycler Staphylococcus MGRADE assay to detect Staphylococcus aureus and coagulase-negative staphylococci in clinical blood samples and in blood culture bottles

Infezioni in Medicina

Volumn 14, Issue 2, 2006, Pages 71-76

  Liberto, Maria Carla ^a   Puccio, Rossana ^a   Matera, Giovanni ^a   Lamberti, Angelo G ^a   Quirino, Angela ^a   Barreca, Giorgio S ^a   Capicotto, Renata ^a   Focà, Alfredo ^a  

^a UNIVERSITY MAGNA GRAECIA OF CATANZARO   (Italy)

Author keywords

Blood samples; CoNS; Real time PCR; Staphylococcus aureus

Indexed keywords

ARTICLE; BACTERIUM DETECTION; BLOOD CULTURE; BLOOD SAMPLING; CLINICAL ARTICLE; COAGULASE NEGATIVE STAPHYLOCOCCUS; COMPUTER PROGRAM; CONTROLLED STUDY; FLUORESCENCE RESONANCE ENERGY TRANSFER; HUMAN; REAL TIME POLYMERASE CHAIN REACTION; STAPHYLOCOCCUS AUREUS;

BACTEREMIA; BLOOD; COAGULASE; COMPUTER SYSTEMS; CULTURE TECHNIQUES; DNA, BACTERIAL; EQUIPMENT CONTAMINATION; FLUORESCENCE RESONANCE ENERGY TRANSFER; HUMANS; NUCLEIC ACID DENATURATION; POLYMERASE CHAIN REACTION; SPECIES SPECIFICITY; STAPHYLOCOCCAL INFECTIONS; STAPHYLOCOCCUS AUREUS; STAPHYLOCOCCUS EPIDERMIDIS; STAPHYLOCOCCUS HAEMOLYTICUS;

EID: 33746321856     PISSN: 11249390     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (13)

1. Hotchkiss R.S., Karl I.E. The pathophysiology and treatment of sepsis. N. Engl. J. Med. 348, 138-150, 2003.
2. Fang H., Hedin G. Rapid screening and identification of methicillin-resistant Staphylococcus aureus from clinical samples by selective-broth and real-time PCR assay. J. Clin. Microbiol. 41, 2894-2899, 2003.
3. Lowy F.D. Staphylococcus aureus infections. N. Engl. J. Med. 339, 520-532, 1998.
4. von Eiff C., Peters G., Heilmann C. Pathogenesis of infections due to coagulase-negative staphylococci. Lancet Infect. Dis. 2, 677-685, 2002.
5. Fatkenheiar G., Preuss M., Salrberger B., et al. Long term outcome and quality of care of patients with Staphylococcus aureus bacteremia. Eur. J. Clin. Microbiol. Infect. Dis. 23, 157-162, 2004.
6. Cockerill F.R., Smith T.F. Rapid-cycle real-time PCR: a revolution for clinical microbiology. ASM News, 68, 77-83, 2003.
7. Shrestha N.K., Tuoby M.J., Hall G.S., Isada C.M., Procop G.W. Rapid identification of Stahylococcus aureus and the mecA gene from BacT/ALERT blood culture bottles by using the LightCycler System. J. Clin. Microbiol. 40, 2659-2661, 2002.
8. Sakai H., Procop G.W., Kobayashi N., et al. Simultaneous detection of Staphylococcus aureus and coagulase-negative staphylococci in positive blood cultures by real-time PCR with two fluorescence resonance energy transfer probe sets. J. Clin. Microbiol. 42, 5739-5744, 2004.
9. Palomares C., Torres M.J., Torres A., Aznar J., Palomares J.C. Rapid detection and identification of Staphylococcus aureus from blood culture specimens using real-time fluorescence PCR. Diagn. Microbiol. Infect. Dis. 45, 183-189, 2003.
10. Tan T.Y., Corden S., Barnes R., Cookson B. Rapid identification of methicillin-resistant Stahylococcus aureus from positive blood culture by real-time fluorenscence PCR. J. Clin. Microbiol. 39, 4529-4531, 2001.
11. Wellinghausen N., Wirths B., Franz A.R., Karolyi L., Marre R., Reischl U. Algorithm for the identification of bacterial pathogens in positive blood cultures by real-time LightCycler polymerase chain reaction (PCR) with sequence-specific probes. Diagn. Microbiol. Infect. Dis. 48, 229-241, 2004.
12. Grisold A.J., Leitner E., Muhlbauer G., Marth E., Kessler H.H. Detection of methicillin-resistant Staphylococcus aureus and simultaneous confirmation by automated nucleic acid extraction and real-time PCR. J. Clin. Microbiol. 40, 2392-2397, 2002.
13. rd. Comparison of the Roche LightCycler vanA/vanB detection assay and culture for detection of vancomycin-resistant enterococci from perianal swabs. J. Clin. Microbiol 42, 2636-2643, 2004.