Co-expression of multiple subunits enables recombinant SNAPC assembly and function for transcription by human RNA polymerases II and III

Protein Expression and Purification

Volumn 48, Issue 2, 2006, Pages 215-223

  Hanzlowsky, Andrej ^a   Jelencic, Blanka ^a   Jawdekar, Gauri ^a   Hinkley, Craig S ^b   Geiger, James H ^a   Henry, R William ^b  

^a MICHIGAN STATE UNIVERSITY   (United States)

^b Michigan State University   (United States)

Author keywords

Co expression; Escherichia coli; Human snRNA; SNAPC; Transcription

Indexed keywords

DNA DIRECTED RNA POLYMERASE III; PROTEIN SUBUNIT; RECOMBINANT PROTEIN; RNA POLYMERASE II; SMALL NUCLEAR RNA; SNAPC PROTEIN COMPLEX, HUMAN; TATA BINDING PROTEIN; TRANSCRIPTION FACTOR;

ARTICLE; CHEMISTRY; ESCHERICHIA COLI; GENETIC TRANSCRIPTION; GENETICS; HUMAN; METABOLISM; MOLECULAR CLONING;

CLONING, MOLECULAR; ESCHERICHIA COLI; HUMANS; PROTEIN SUBUNITS; RECOMBINANT PROTEINS; RNA POLYMERASE II; RNA POLYMERASE III; RNA, SMALL NUCLEAR; TATA-BOX BINDING PROTEIN; TRANSCRIPTION FACTORS; TRANSCRIPTION, GENETIC;

ESCHERICHIA COLI;

EID: 33746257485     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2006.02.015     Document Type: Article

References (32)

1. Roeder R.G. Transcriptional regulation and the role of diverse coactivators in animal cells. FEBS Lett. 579 (2005) 909-915
2. Albright S.R., and Tjian R. TAFs revisited: more data reveal new twists and confirm old ideas. Gene 242 (2000) 1-13
3. Tora L. A unified nomenclature for TATA box binding protein (TBP)-associated factors (TAFs) involved in RNA polymerase II transcription. Genes Dev. 16 (2002) 673-675
4. Geiduschek E.P., and Kassavetis G.A. Comparing transcriptional initiation by RNA polymerases I and III. Curr. Opin. Cell Biol. 7 (1995) 344-351
5. Paule M.R., and White R.J. Survey and summary: transcription by RNA polymerases I and III. Nucleic Acids Res. 28 (2000) 1283-1298
6. Sadowski C.L., Henry R.W., Lobo S.M., and Hernandez N. Targeting TBP to a non-TATA box cis-regulatory element: a TBP-containing complex activates transcription from snRNA promoters through the PSE. Genes Dev. 7 (1993) 1535-1548
7. Murphy S., Yoon J.B., Gerster T., and Roeder R.G. Oct-1 and Oct-2 potentiate functional interactions of a transcription factor with the proximal sequence element of small nuclear RNA genes. Mol. Cell. Biol. 12 (1992) 3247-3261
8. Henry R.W., Ford E., Mital R., Mittal V., and Hernandez N. Crossing the line between RNA polymerases: transcription of human snRNA genes by RNA polymerases II and III. Cold Spring Harb. Symp. Quant. Biol. 63 (1998) 111-120
9. Hernandez N. Small nuclear RNA genes: a model system to study fundamental mechanisms of transcription. J. Biol. Chem. 276 (2001) 26733-26736
10. Mittal V., and Hernandez N. Role for the amino-terminal region of human TBP in U6 snRNA transcription. Science 275 (1997) 1136-1140
11. Schramm L., Pendergrast P.S., Sun Y., and Hernandez N. Different human TFIIIB activities direct RNA polymerase III transcription from TATA-containing and TATA-less promoters. Genes Dev. 14 (2000) 2650-2663
12. Kuhlman T.C., Cho H., Reinberg D., and Hernandez N. The general transcription factors IIA, IIB, IIF, and IIE are required for RNA polymerase II transcription from the human U1 small nuclear RNA promoter. Mol. Cell. Biol. 19 (1999) 2130-2141
13. C, and DNA. J. Biol. Chem. 272 (1997) 16048-16055
14. C largest subunit that bears sequence similarities to the Oct-1 coactivator OBF-1. Genes Dev. 12 (1998) 3528-3540
15. Mittal V., Ma B., and Hernandez N. SNAP(c): a core promoter factor with a built-in DNA-binding damper that is deactivated by the Oct-1 POU domain. Genes Dev. 13 (1999) 1807-1821
16. Hovde S., Hinkley C.S., Strong K., Brooks A., Gu L., Henry R.W., and Geiger J. Activator recruitment by the general transcription machinery: X-ray structural analysis of the Oct-1 POU domain/human U1 octamer/SNAP190 peptide ternary complex. Genes Dev. 16 (2002) 2772-2777
17. C) shared by RNA polymerases II and III. Genes Dev. 12 (1998) 2664-2672
18. Henry R.W., Sadowski C.L., Kobayashi R., and Hernandez N. A TBP-TAF complex required for transcription of human snRNA genes by RNA polymerase II and III. Nature 374 (1995) 653-656
19. Yoon J.B., and Roeder R.G. Cloning of two proximal sequence element-binding transcription factor subunits (gamma and delta) that are required for transcription of small nuclear RNA genes by RNA polymerases II and III and interact with the TATA-binding protein. Mol. Cell. Biol. 16 (1996) 1-9
20. Sadowski C.L., Henry R.W., Kobayashi R., and Hernandez N. The SNAP45 subunit of the small nuclear RNA (snRNA) activating protein complex is required for RNA polymerase II and III snRNA gene transcription and interacts with the TATA box binding protein. Proc. Natl. Acad. Sci. USA 93 (1996) 4289-4293
21. C. EMBO J. 15 (1996) 7129-7136
22. Bai L., Wang Z., Yoon J.B., and Roeder R.G. Cloning and characterization of the beta subunit of human proximal sequence element-binding transcription factor and its involvement in transcription of small nuclear RNA genes by RNA polymerases II and III. Mol. Cell. Biol. 16 (1996) 5419-5426
23. C is a Myb domain protein that interacts with Oct-1. Mol. Cell. Biol. 18 (1998) 368-377
24. C. J. Biol. Chem. 276 (2001) 5027-5035
25. Ma B., and Hernandez N. Redundant cooperative interactions for assembly of a human U6 transcription initiation complex. Mol. Cell. Biol. 22 (2002) 8067-8078
26. Hinkley C.S., Hirsch H.A., Gu L., LaMere B., and Henry R.W. The SNAP190 Myb DNA binding domain stimulates TBP-TATA box recognition. J. Biol. Chem. 278 (2003) 18649-18657
27. Hirsch H.A., Gu L., and Henry R.W. The retinoblastoma tumor suppressor protein targets distinct general transcription factors to regulate RNA polymerase III gene expression. Mol. Cell. Biol. 20 (2000) 9182-9191
28. Hirsch H.A., Jawdekar G.W., Lee K.A., Gu L., and Henry R.W. Distinct mechanisms for repression of RNA polymerase III transcription by the Retinoblastoma tumor suppressor protein. Mol. Cell. Biol. 24 (2004) 5989-5999
29. Gridasova A.A., and Henry R.W. The p53 tumor suppressor protein represses human snRNA gene transcription by RNA polymerases II and III independently of sequence-specific DNA binding. Mol. Cell. Biol. 25 (2005) 3247-3260
30. C. Mol. Cell. Biol. 16 (1996) 1955-1965
31. C. J. Biol. Chem. 280 (2005) 27697-27704
32. Tolia N.H., and Joshua-Tor L. Strategies for protein coexpression in Escherichia coli. Nat. Methods 3 (2006) 55-64