A new dermal equivalent: The use of dermal fibroblast culture alone without exogenous materials

Journal of Dermatological Science

Volumn 43, Issue 2, 2006, Pages 95-104

  Lee, Dong Youn ^a   Lee, Joo Heung ^a   Yang, Jun Mo ^a   Lee, Eil Soo ^a   Park, Kwan Hyun ^a   Mun, Goo Hyun ^a  

^a Sungkyunkwan University School of Medicine   (South Korea)

Author keywords

Culture; Dermal equivalent; Fibroblasts; Graft; Keratinocytes; Skin equivalent

Indexed keywords

BETA4 INTEGRIN; COLLAGEN FIBER; COLLAGEN TYPE 1; COLLAGEN TYPE 3; COLLAGEN TYPE 4; COLLAGEN TYPE 7; CYTOKERATIN 14; ELASTIN; EPIDERMAL GROWTH FACTOR; FIBRILLIN 1; FIBRONECTIN; HYDROCORTISONE; INSULIN; INVOLUCRIN; LIOTHYRONINE; TRANSFERRIN;

AIR; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BASEMENT MEMBRANE; BLOOD VESSEL; CELL CULTURE; CULTURE MEDIUM; DERMIS; ELECTRON MICROSCOPY; EVALUATION; EXTRACELLULAR MATRIX; HEMIDESMOSOME; HISTOLOGY; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; IN VITRO STUDY; IN VIVO STUDY; KERATINOCYTE; LIQUID; MALE; MOUSE; MOUSE STRAIN; NONHUMAN; PRIORITY JOURNAL; SKIN; SKIN FIBROBLAST; SKIN GRAFT; STAINING; TRANSMISSION ELECTRON MICROSCOPY; ULTRASTRUCTURE;

ANIMALS; CELLS, CULTURED; EPIDERMIS; FIBROBLASTS; HUMANS; KERATINOCYTES; MICE; MICE, NUDE; SKIN; SKIN TRANSPLANTATION; SKIN, ARTIFICIAL; TRANSPLANTATION, HETEROLOGOUS;

EID: 33746192498     PISSN: 09231811     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jdermsci.2006.03.007     Document Type: Article

References (42)

1. Regnier M., Prunieras M., and Woodley D. Growth and differentiation of adult human epidermal cells on dermal substrates. Front Matrix Biol 9 (1981) 4-35
2. Bell E., Sher S., Hull B., Merrill C., Rosen S., Chamson A., et al. The reconstitution of living skin. J Invest Dermatol 81 (1983) 2s-10s
3. Boyce S.T., Michel S., Reichert U., Schroot B., and Schmidt R. Reconstructed skin from cultured human keratinocytes and fibroblasts on a collagen-glycosaminoglycan biopolymer substrate. Skin Pharmacol 2 (1990) 136-143
4. Maruguchi T., Maruguchi Y., Suzuki S., Matsuda K., Toda K.I., and Isshiki N. A new skin equivalent: keratinocytes proliferated and differentiated on collagen sponge containing fibroblasts. Plast Reconstr Surg 93 (1994) 537-544
5. Lee D.Y., Ahn H.T., and Cho K.H. A new skin equivalent model: use of a dermal substrate which combines de-epidermized dermis with fibroblast-populated collagen matrix. J Dermatol Sci 23 (2000) 132-137
6. Lee D.Y., and Cho K.H. The effects of epidermal keratinocytes and dermal fibroblasts on the formation of cutaneous basement membrane in three-dimensional culture systems. Arch Dermatol Res 296 (2005) 296-302
7. Green H., Kehinde O., and Thomas J. Growth of cultured human epidermal cells into multiple epithelia suitable for grafting. Proc Natl Acad Sci USA 76 (1979) 5665-5668
8. Regnier M., Asselineau D., and Lenoir M.C. Human epidermis reconstructed on dermal substrates in vitro: an alternative to animals in skin pharmacology. Skin Pharmacol 3 (1990) 70-85
9. Ponec M. Reconstruction of human epidermis on de-epidermized dermis: expression of differentiation-specific protein markers and lipid composition. Toxicol In Vitro 5 (1991) 597-606
10. Gay R., Swiderek M., Nelson D., and Ernesti A. The living skin equivalent as in vitro for ranking the toxic potential of dermal irritants. Toxic In Vitro 6 (1992) 303-315
11. Ehrlich H.P. Understanding experimental biology of skin equivalent: from laboratory to clinical use in patients with burns and chronic wounds. Am J Surg 187 (2004) 29s-33s
12. Cooper M.L., Hansbrough J.F., Spielvogel R.L., Cohen R., Bartel R.L., and Naughton G. In vivo optimization of a living dermal substitute employing cultured human fibroblasts on a biodegradable polyglycolic acid or polyglactin mesh. Biomaterials 12 (1991) 243-248
13. Bettger W.J., Boyce S.T., Walthall B.J., and Ham R.G. Rapid clonal growth and serial passage of human diploid fibroblasts in a lipid-enriched synthetic medium supplemented with epidermal growth factor, insulin, and dexamethasone. Proc Natl Acad Sci USA 78 (1981) 5588-5592
14. Walthall B.J., and Ham R.G. Multiplication of human diploid fibroblasts in a synthetic medium supplemented with EGF, insulin, and dexamethasone. Exp Cell Res 134 (1981) 303-311
15. Goldstein R.H., Poliks C.F., Pilch P.F., Smith B.D., and Fine A. Stimulation of collagen formation by insulin and insulin-like growth factor I in cultures of human lung fibroblasts. Endocrinology 124 (1989) 964-970
16. Carpenter G., and Cohen S. Epidermal growth factor. Annu Rev Biochem 48 (1979) 193-216
17. Russell S.B., Russell J.D., and Trupin K.M. Collagen synthesis in human fibroblasts: effects of ascorbic acid and regulation by hydrocortisone. J Cell Physiol 109 (1981) 121-131
18. Hata R., Sunada H., Arai K., Sato T., Ninomiya Y., Nagai Y., et al. Regulation of collagen metabolism and cell growth by epidermal growth factor and ascorbate in cultured human skin fibroblasts. Eur J Biochem 173 (1988) 261-267
19. Booth B.A., Polak K.L., and Uitto J. Collagen biosynthesis by human skin fibroblasts. I. Optimization of the culture conditions for synthesis of type I and type III procollagens. Biochim Biophys Acta 607 (1980) 145-160
20. Minor R.R., Sippola-Thiele M., McKeon J., Berger J., and Prockop D.J. Defects in the processing of procollagen to collagen are demonstrable in cultured fibroblasts from patients with the Ehlers-Danlos and osteogenesis imperfecta syndromes. J Biol Chem 261 (1986) 10006-10014
21. Boyce S.T., and Ham R.G. Calcium-regulated differentiation of normal human epidermal keratinocytes in chemically defined clonal culture. J Invest Dermatol 81 (1983) 33s-40s
22. Lee D.Y., Lee J.H., Lee E.S., Cho K.H., and Yang J.M. Fibroblasts play a stimulatory role in keratinocyte proliferation but an inhibitory role in melanocyte growth and pigmentation in a skin equivalent system from skin type IV. Arch Dermatol Res 294 (2003) 444-446
23. Barlow Y., and Pye R.J. Keratinocyte culture. Methods Mol Biol 75 (1997) 117-129
24. Kurata S., and Hata R. Epidermal growth factor inhibits transcription of type I collagen genes and production of type I collagen in cultured human skin fibroblasts in the presence and absence of l-ascorbic acid 2-phosphate, a long-acting vitamin C derivative. J Biol Chem 266 (1991) 9997-10003
25. Colige A., Nusgens B., and Lapiere C.M. Effect of EGF on human skin fibroblasts is modulated by the extracellular matrix. Arch Dermatol Res 280 (1988) S42-S46
26. Krupsky M., Fine A., Kuang P.P., Berk J.L., and Goldstein R.H. Regulation of type I collagen production by insulin and transforming growth factor-beta in human lung fibroblasts. Connect Tissue Res 34 (1996) 53-62
27. Kan M., and Yamane I. In vitro proliferation and lifespan of human diploid fibroblasts in serum-free BSA-containing medium. J Cell Physiol 111 (1982) 155-162
28. Kan M., and Yamane I. Effects of ferrous iron and transferrin on cell proliferation of human diploid fibroblasts in serum-free culture. In Vitro 20 (1984) 89-94
29. Skinner S.J., Ashby C.J., and Liggins G.C. Transferrin stimulates proteoglycan accumulation by fetal lung cells in culture. Exp Lung Res 15 (1989) 269-283
30. Shishiba Y., Takeuchi Y., Yokoi N., Ozawa Y., and Shimizu T. Thyroid hormone excess stimulates the synthesis of proteoglycan in human skin fibroblasts in culture. Acta Endocrinol 123 (1990) 541-549
31. de Rycker C., Vandalem J.L., and Hennen G. Effects of 3,5,3′-triiodothyronine on collagen synthesis by cultured human skin fibroblasts. FEBS Lett 174 (1984) 34-37
32. Narayanan A.S., and Page R.C. Serum regulation of collagen biosynthesis in human diploid fibroblasts. Biochem Biophys Res Commun 145 (1987) 639-645
33. Grinnell F., Fukamizu H., Pawelek P., and Nakagawa S. Collagen processing, crosslinking, and fibril bundle assembly in matrix produced by fibroblasts in long-term cultures supplemented with ascorbic acid. Exp Cell Res 181 (1989) 483-491
34. L'Heureux N., Paquet S., Labbe R., Germain L., and Auger F.A. A completely biological tissue-engineered human blood vessel. FASEB J 12 (1998) 47-56
35. Murad S., Tajima S., Johnson G.R., Sivarajah S., and Pinnell S.R. Collagen synthesis in cultured human skin fibroblasts: effect of ascorbic acid and its analogs. J Invest Dermatol 81 (1983) 158-162
36. Geesin J., Murad S., and Pinnell S.R. Ascorbic acid stimulates collagen production without altering intracellular degradation in cultured human skin fibroblasts. Biochim Biophys Acta 886 (1986) 272-274
37. Peterkofsky B. The effect of ascorbic acid on collagen polypeptide synthesis and proline hydroxylation during the growth of cultured fibroblasts. Arch Biochem Biophys 152 (1972) 318-328
38. Hata R., and Senoo H. l-Ascorbic acid 2-phosphate stimulates collagen accumulation, cell proliferation, and formation of a three-dimensional tissue like substance by skin fibroblasts. J Cell Physiol 138 (1989) 8-16
39. Chung J.H., Cho K.H., Lee D.Y., Kwon O.S., Sung M.W., Kim K.H., et al. Human oral buccal mucosa reconstructed on dermal substrates: a model for oral epithelial differentiation. Arch Dermatol Res 289 (1997) 677-685
40. El Ghalbzouri A., and Ponec M. Diffusible factors released by fibroblasts support epidermal morphogenesis and deposition of basement membrane components. Wound Repair Regen 12 (2004) 359-367
41. Pouliot R., Larouche D., Auger F.A., Juhasz J., Xu W., Li H., et al. Reconstructed human skin produced in vitro and grafted on athymic mice. Transplantation 73 (2002) 1751-1757
42. Demarchez M., Hartmann D.J., Regnier M., and Asselineau D. The role of fibroblasts in dermal vascularization and remodeling of reconstructed human skin after transplantation onto the nude mouse. Transplantation 54 (1992) 317-326