A new skin equivalent model: Dermal substrate that combines de-epidermized dermis with fibroblast-populated collagen matrix

Journal of Dermatological Science

Volumn 23, Issue 2, 2000, Pages 132-137

  Lee, Dong Youn ^a   Ahn, Hi Tae ^a   Cho, Kwang Hyun ^a  

^a Seoul National University Hospital   (South Korea)

Author keywords

DED; Differentiation; Fibroblast; Skin equivalent

Indexed keywords

COLLAGEN; COLLAGEN TYPE 1; FILAGGRIN; INVOLUCRIN; LORICRIN;

ARTICLE; BIOLOGICAL MODEL; CELL DIFFERENTIATION; COLLAGEN METABOLISM; EPIDERMIS; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; KERATINOCYTE; PREPUCE; PRIORITY JOURNAL; SKIN FIBROBLAST;

CELLS, CULTURED; COLLAGEN; DERMIS; EPIDERMIS; FIBROBLASTS; HUMANS; IMMUNOHISTOCHEMISTRY; INFANT, NEWBORN; KERATINOCYTES; MALE; SKIN; SKIN, ARTIFICIAL;

EID: 0034212153     PISSN: 09231811     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0923-1811(00)00068-2     Document Type: Article

References (28)

1. Regnier M., Prunieras M., Woodley D. Growth and differentiation of adult human epidermal cells on dermal substrates. Front. Matrix Biol. 9:1981;4-35.
2. Bell E., Sher S., Hull B., Merrill C., Rosen S., Chamson A., Asselineau D., Dubertret L., Coulomb B., Lapiere C., Nusgens B., Neveux Y. The reconstitution of living skin. J. Invest. Dermatol. 81:1983;2s-10s.
3. Regnier M., Asselineau D., Lenoir M.C. Human epidermis reconstructed on dermal substrates in vitro: an alternative to animals in skin pharmacology. Skin Pharmacol. 3:1990;70-85.
4. Fartasch M., Ponec M. Improved barrier structure formation in air-exposed human keratinocyte culture system. J. Invest. Dermatol. 102:1994;366-374.
5. Asselineau D., Bernard B.A., Bailly C., Darmon M., Prunieras M. Human epidermis reconstructed by culture: is it `normal'? J. Invest. Dermatol. 86:1986;181-186.
6. Ponec M. Reconstruction of human epidermis on de-epidermized dermis: expression of differentiation-specific protein markers and lipid composition. Toxicol. Vitro. 5:1991;597-606.
7. Bodde H.E., Holman B., Spies F., Weerheim A., Kempernaar J., Mommaas A.M., Ponec M. Freeze-fracture electron microscopy of in vitro reconstructed epidermis. J. Invest. Dermatol. 95:1990;108-116.
8. Bouwstra J.A., Gooris G.S., Spek J.A., Bras W. Structural investigations of human stratum corneum by small-angle X-ray scattering. J. Invest. Dermatol. 97:1991;1007-1012.
9. Mak V.H.W., Cumpstone M.B., Kennedy A.H., Harmon C.S., Guy R.H., Potts R.O. Barrier function of human keratinocyte cultures grown at the air-liquid interface. J. Invest. Dermatol. 96:1991;323-327.
10. Regnier M., Caron D., Reichert U., Schaefer H. Reconstructed human epidermis: a model to study in vitro the barrier function of the skin. Skin Pharmacol. 5:1992;49-56.
11. Bosca A.R., Tinois E., Faure M., Kanitakis J., Roche P., Thivolet J. Epithelial differentiation of human skin equivalents after grafting onto nude mice. J. Invest. Dermatol. 91:1988;136-141.
12. Higounenc I., Demarchez M., Regnier M., Schmidt R., Ponec M., Shroot B. Improvement of epidermal differentiation and barrier function in reconstructed human skin after grafting onto athymic nude mice. Arch. Dermatol. Res. 286:1994;107-114.
13. Ponec M., Weerheim A., Kempenaar J., Mulder A., Gooris G.S., Bouwstra J., Mommaas A.M. The formation of competent barrier lipids in reconstructed human epidermis requires the presence of vitamin C. J. Invest. Dermatol. 109:1997;348-355.
14. Ponec M., Gibbs S., Weerheim A., Kempenaar J., Mulder A., Mommaas A.M. Epidermal growth factor and temperature regulate keratinocyte differentiation. Arch. Dermatol. Res. 289:1997;317-326.
15. Gibbs S., Vicanova J., Bouwstra J., Valstar C., Kempenaar J., Ponec M. Culture of reconstructed epidermis in a defined medium at 33°C shows a delayed epidermal maturation, prolonged lifespan and improved stratum corneum. Arch. Dermatol. Res. 289:1997;585-595.
16. Gibbs S., Boelsma E., Kempenaar J., Ponec M. Temperature-sensitive regulation of epidermal morphogenesis and the expression of cornified envelope precursors by EGF and TGFα Cell Tissue Res. 292:1998;107-114.
17. Krejci N.C., Cuono C.B., Langdon R.C., McGuire J. In vitro reconstitution of skin: fibroblasts facilitate keratinocyte growth and differentiation on acellular reticular dermis. J. Invest. Dermatol. 97:1991;843-848.
18. Smola H., Thiekotter G., Fusenig N.E. Mutual induction of growth factor gene expression by epidermal-dermal interaction. J. Cell Biol. 122:1993;417-429.
19. Maruguchi T., Maruguchi Y., Suzuki S., Matsuda K., Toda K.I., Isshiki N. A new skin equivalent: keratinocytes proliferated and differentiated on collagen sponge containing fibroblasts. Plast. Reconstr. Surg. 93:1994;537-544.
20. Ghosh M.M., Boyce S., Layton C., Freedlander E., Neil S.M. A comparison of methodologies for the preparation of human epidermal-dermal composites. Ann. Plast. Surg. 39:1997;390-404.
21. Ralston D.R., Layton C., Dalley A.J., Boyce S.G., Freedlander E., Neil S.M. The requirement for basement membrane antigens in the production of human epidermal/dermal composites in vitro. Br. J. Dermatol. 140:1999;605-615.
22. Schoop V.M., Mirancea N., Fusenig N.E. Epidermal organization and differentiation of HaCaT keratinocytes in organotypic coculture with human dermal fibroblasts. J. Invest. Dermatol. 112:1999;343-353.
23. Boyce S.T., Ham R.G. Calcium-regulated differentiation of normal human epidermal keratinocytes in chemically defined clonal culture. J. Invest. Dermatol. 81:1983;33s-40s.
24. Kopan R., Traska G., Fuchs E. Retinoids as important regulators of terminal differentiation: examining keratin expression in individual epidermal cells at various stages of keratinization. J. Cell Biol. 105:1987;427-440.
25. Chung J.H., Cho K.H., Lee D.Y., Kwon O.S., Sung M.W., Kim K.H., Eun H.C. Human oral buccal mucosa reconstructed on dermal substrates: a model for oral epithelial differentiation. Arch. Dermatol. Res. 289:1997;677-685.
26. Limat A., Breitkreutz D., Hunziker T., Boillat C., Wiesmann U., Klein E., Noser F., Fusenig N.E. Restoration of the epidermal phenotype by follicular outer root sheath cells in recombinant culture with fibroblast. Exp. Cell Res. 194:1991;218-227.
27. Saintigny G., Bonnard M., Damour O., Collombel C. Reconstruction of epidermis on a chitosan cross-linked collagen-GAG lattice: effect of fibroblasts. Acta Derm. Venereol. 73:1993;175-180.
28. Medalie D.A., Eming S.A., Tompkins R.G., Yarmush M.L., Krueger G.G., Morgan J.R. Evaluation of human skin reconstituted from composite grafts of cultured keratinocytes and human acellular dermis transplantation to athymic mice. J. Invest. Dermatol. 107:1996;121-127.