Metabolic profiling of laser microdissected vascular bundles of Arabidopsis thaliana

Plant Methods

Volumn 1, Issue 1, 2005, Pages

  Schad, Martina ^a   Mungur, Rajsree ^a   Fiehn, Oliver ^b   Kehr, Julia ^a  

^a MAX PLANCK INSTITUTE OF MOLECULAR PLANT PHYSIOLOGY   (Germany)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

GC TOF MS; laser microdissection; metabolite profiling; tissue specific analysis; vascular bundle

Indexed keywords

EID: 33745944637     PISSN: None     EISSN: 17464811     Source Type: Journal    
DOI: 10.1186/1746-4811-1-2     Document Type: Article

References (27)

1. Tomos AD, Hinde P, Pritchard J, Fricke W. Microsampling and measurements of solutes in single cells. Plant cell biology: a practical approach Edited by Harris N, Oparka KJ Oxford: Oxford University Press 1994, 297-314.
2. Andersson H, van den Berg A. Microtechnologies and nanotechnologies for single-cell analysis. Current Opinion in Biotechnology 2004, 15:44-49.
3. Brandt S, Kehr J, Walz C, Imlau A, Willmitzer L, Fisahn J. A rapid method for detection of plant gene transcripts from single epidermal, mesophyll and companion cells of intact leaves. Plant Journal 1999, 20:245-250. 10.1046/j.1365-313x.1999.00583.x, 10571884.
4. Arlt K, Brandt S, Kehr J. Amino acid analysis in five pooled single plant cell samples using capillary electrophoresis coupled to laser-induced fluorescence detection. Journal of Chromatography A 2001, 926:319-325. 10.1016/S0021-9673(01)01052-4, 11556336.
5. Tomos AD, Sharrock RA. Cell sampling and analysis (SiCSA): metabolites measured at single cell resolution. Journal of Experimental Botany 2001, 52:623-630. 10.1093/jexbot/52.356.623, 11373310.
6. Schuetze K, Lahr G. Identification of expressed genes by laser-mediated manipulation of single cells. Nat Biotech 1998, 16:737-742.
7. Emmert-Buck M, Bonner R, Smith P, Chuaqui R, Zhuang Z, Goldstein S, Weiss R, Liotta L. Laser capture microdissection. Science 1996, 274:998-1001. 10.1126/science.274.5289.998, 8875945.
8. Wittliff JL, Erlander MG. Laser capture microdissection and its applications in genomics and and proteomics. Methods in Enzymology 2002, 356:12-25.
9. Westphal G, Friedemann RB, Wellmann A, Wernert N, Wollscheid V, Becker B, Vogt T, Knuchel R, Stolz W, Schutze K. Noncontact laser catapulting: a basic procedure for functional genomics and proteomics. Methods in Enzymology 2002, 356:80-99.
10. Kerk NM, Ceserani T, Tausta SL, Sussex IM, Nelson TM. Laser capture microdissection of cells from plant tissues. Plant Physiology 2003, 132:27-35. 10.1104/pp.102.018127, 12746508.
11. Asano T, Masumura T, Kusano H, Kikuchi S, Kurita A, Shimada H, Kadowaki K. Construction of a specialized cDNA library from plant cells isolated by laser capture microdissection: toward comprehensive analysis of the genes expressed in the rice phloem. Plant Journal 2002, 32:401-408. 10.1046/j.1365-313X.2002.01423.x, 12410817.
12. Nakazono M, Qiu F, Borsuk LA, Schnable PS. Laser-capture microdissection, a tool for the global analysis of gene expression in specific plant cell types: identification of genes expressed differentially in epidermal cells or vascular tissues of maize. Plant Cell 2003, 15:583-596. 150015, 12615934, 10.1105/tpc.008102.
13. Schad M, Lipton MS, Giavalisco P, Smith RD, Kehr J. Evaluation of 2-DE and LC-MS/MS for tissue-specific protein profiling of laser microdissected plant samples. Electrophoresis 2005,
14. Ahram M. Evaluation of ethanol-fixed, paraffin-embedded tissues for proteomic applications. Proteomics 2003, 3:413. 10.1002/pmic.200390056, 12687609.
15. Gillespie JW, Best CJM, Emmert-Buck MR. Evaluation of non-formalin tissue fixation for molecular profiling studies. American Journal of Pathology 2002, 160:449-457.
16. Ge H, Gong X, Tang CK. Evidence of high incidence of EGFRvIII expression and coexpression with EGFR in human invasive breast cancer by laser capture microdissection and immunohistochemical analysis. International Journal of Cancer 2002, 98:357-361.
17. Upson JJ, Stoyanova R, Cooper HS, Patriotis C, Ross EA, Boman B, Clapper ML, Knudson AG, Bellacosa A. Optimized procedures for microarray analysis of histological specimens processed by laser capture microdissection. Journal of Cellular Physiology 2004, 201:366-373.
18. Kehr J. High resolution spatial analysis of plant systems. Current Opinion in Plant Biology 2001, 197-201. 10.1016/S1369-5266(00)00161-8, 11312129.
19. Kehr J. Single cell technology. Current Opinion in Plant Biology 2003, 6:1-5.
20. Burgemeister R, Gangnus R, Haar B, Schütze K, Sauer U. High quality RNA retrieved from samples obtained by using LMPC (Laser Microdissection and Pressure Catapulting) technology. Pathology Research and Practice 2003, 199:431-436.
21. Brandt S, Walz C, Schad M, Pavlovic N, Kehr J. A simple, chisel assisted mechanical microdissection system for harvesting homogenous plant tissue suitable for the analysis of nucleic acids and proteins. Plant Molecular Biology Reporter 2003, 21:417-427.
22. Fellenberg J, Krauthoff A, Pollandt K, Delling G, Parsch D. Evaluation of the predictive value of Her-2/neu gene expression on osteosarcoma therapy in laser-microdissected paraffin-embedded tissue. Laboratory Investigation 2004, 84:113-121. 10.1038/sj.labinvest.3700006, 14631380.
23. Kim JO, Kim HN, Hwang MH, Shin HI, Kim SY, Park RW, Park EY, Kim IS, Wijnen AJ, Stein JL, Lian JB, Stein GS, Choi JY. Differential gene expression analysis using paraffin-embedded tissues after laser microdissection. Journal of Cellular Biochemistry 2003, 90:998-1006.
24. Kehr J, Hustiak F, Walz C, Willmitzer L, Fisahn J. Transgenic plants changed in carbon allocation pattern display a shift in diurnal growth pattern. Plant Journal 1998, 16:497-503. 10.1046/j.1365-313x.1998.00318.x, 9881169.
25. Weckwerth W, Loureiro ME, Wenzel K, Fiehn O. Differential metabolic networks unravel the effects of silent plant phenotypes. PNAS 2004, 101:7809-7814. 419688, 15136733, 10.1073/pnas.0303415101.
26. Weckwerth W, Wenzel K, Fiehn O. Process for the integrated extraction, identification and quantification of metabolites, proteins and RNA to reveal their co-regulation in biochemical networks. Proteomics 2004, 4:78-83.
27. Zhu X, Shaw PN, Pritchard J, Newbury J, Hunt EJ, Barrett DA. Amino acid analysis by micellar electrokinetic chromatography with laser-induced fluorescence detection: Application to nanolitre-volume biological samples from Arabidopsis thaliana and Myzus persicae. Electrophoresis 2005, 26:911-919. 10.1002/elps.200410259, 15714547.