Automated high-throughput process for site-directed mutagenesis, production, purification, and kinetic characterization of enzymes

Analytical Biochemistry

Volumn 355, Issue 1, 2006, Pages 110-116

  Carapito, Raphaël ^a   Gallet, Benoit ^a   Zapun, André ^a   Vernet, Thierry ^a  

^a INSTITUT DE BIOLOGIE STRUCTURALE   (France)

Author keywords

Automation; Enzyme kinetics; High throughput; Mutagenesis; Protein purification

Indexed keywords

AUTOMATION; ENZYME KINETICS; MUTAGENESIS; PURIFICATION;

AUTOMATED LIQUID HANDLING; FUNCTIONAL CHARACTERIZATION; HIGH THROUGHPUT; KINETIC CHARACTERIZATION; PENICILLIN- BINDING PROTEINS; PROTEIN PURIFICATION; SITE DIRECTED MUTAGENESIS; STRUCTURE-FUNCTION RELATIONSHIP;

THROUGHPUT;

PENICILLIN BINDING PROTEIN; PENICILLIN BINDING PROTEIN 2X; UNCLASSIFIED DRUG;

ARTICLE; AUTOMATION; ENZYME ANALYSIS; ENZYME KINETICS; ENZYME STRUCTURE; ENZYME SYNTHESIS; HIGH THROUGHPUT SCREENING; NONHUMAN; PRIORITY JOURNAL; PROTEIN FUNCTION; PROTEIN PURIFICATION; SITE DIRECTED MUTAGENESIS; STREPTOCOCCUS PNEUMONIAE;

STREPTOCOCCUS PNEUMONIAE;

EID: 33745897335     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2006.04.047     Document Type: Article

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