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Combining data from numerous laboratories working on many different species of vertebrates, the authors propose, for the first time, a comprehensive overview of the neural crest regulatory network. In this way, they provide a backbone for further refinement of the molecular description of neural crest formation from a gene regulatory standpoint. This suggests a possible mechanism of neural crest evolution through the elaborations of this regulatory state.
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Meulemans D., and Bronner-Fraser M. Gene-regulatory interactions in neural crest evolution and development. Dev Cell 7 (2004) 291-299. Combining data from numerous laboratories working on many different species of vertebrates, the authors propose, for the first time, a comprehensive overview of the neural crest regulatory network. In this way, they provide a backbone for further refinement of the molecular description of neural crest formation from a gene regulatory standpoint. This suggests a possible mechanism of neural crest evolution through the elaborations of this regulatory state.
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The authors show that co-activation of the neural plate border specifiers Pax3 and Zic1 is essential for neural crest specification, as assessed by expression of neural crest markers FoxD3 and Snail2, and requires Wnt signaling.
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The authors show that the small helix-loop-helix protein Id3 is a direct target of c-Myc. Its depletion in Xenopus embryos leads to failure of neural crest development and excess formation of central nervous system progenitors, indicating a cell fate switch. By contrast, prolonged expression maintains the neural crest in an extended multipotent progenitor state.
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The authors show that Snail is post-transcriptionally regulated by GSK3β. The Snail protein is dually regulated by GSK3β, which binds to and phosphorylates it at two consecutive consensus motifs, thereby controlling the nuclear export of the protein and its subsequent βTrcp-mediated ubiquitination.
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The authors show that Sox9 and Sox10, two members of the SoxE transcription factor family, might undergo post-translational modification by sumoylation. Modified and non-modified SoxE proteins might mediate different subsets of transcriptional activities in either neural crest specification or otic placode formation.
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