Protein adsorption and hydrodynamic stability of a dense, pellicular adsorbent in high-biomass expanded bed chromatography

Biotechnology and Bioprocess Engineering

Volumn 11, Issue 3, 2006, Pages 268-272

  Chow, Yen Mei ^a   Tey, Beng Ti ^a   Ibrahim, Mohd Nordin ^a   Ariff, Arbakariya ^a   Ling, Tau Chuan ^a  

^a UNIVERSITY PUTRA MALAYSIA   (Malaysia)

Author keywords

Bovine serum albumin; Expanded bed chromatography; Hydrodynamic stability; Protein adsorption; Yeast cells

Indexed keywords

ADSORBENT; BOVINE SERUM ALBUMIN; CIBACRON BLUE F3GA;

ADSORPTION KINETICS; ANIMAL CELL; ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; CORRELATION COEFFICIENT; EXPANDED BED CHROMATOGRAPHY; HYDRODYNAMICS; MEAN RESIDENCE TIME; NONHUMAN; PROTEIN STABILITY; YEAST CELL;

BOS TAURUS;

EID: 33745615827     PISSN: 12268372     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02932042     Document Type: Article

References (24)

1. Chow, Y. M., M. N. Ibrahim, B. T. Tey, A. B. Ariff, and T. C. Ling (2005) The influence of bakers' yeast cells on protein adsorption in anion exchange expanded bed chromatography. Biotechnol. Bioprocess Eng. 10: 280-283.
2. Chow, Y. M., M. N. Ibrahim, B. T. Tey, A. B. Ariff, and T. C. Ling (2005) The influence of bakers' yeast cells on protein adsorption in dye-ligand expanded bed chromatography. Biotechnol. Bioprocess Eng. 10: 552-555.
3. Ling, T. C., C. K. Loong, W. S. Tan, B. T. Tey, W. M. W. Abdullah, and A. Ariff (2004) Purification of filamentous bacteriophage M13 by expanded bed anion exchange chromatography. J. Microbiol. 42: 228-232.
4. Tan, Y. P., T. C. Ling, K. Yusoff, W. S. Tan, and B. T. Tey (2005) Comparative evaluation of three purification methods for the nucleocapsid protein of Newcastle disease virus from Escherichia coli homogenates. J. Microbiol. 43: 295-300.
5. Thömmes J., M. Halfar, S. Lenz, and M. R. Kula (1995) Purification of monoclonal antibodies from whole hybridoma fermentation broth by fluidized bed adsorption. Biotechnol. Bioeng. 45: 205-211.
6. Hjorth, R. (1997) Expanded-bed adsorption in industrial bioprocessing: recent developments. Trends Biotechnol. 15: 230-235.
7. Ling, T. C. and A. Lyddiatt (2005) Process intensification of fluidized bed dye-ligand adsorption of G3PDH from unclarified disrupted yeast: a case study of the performance of a high-density steel-agarose pellicular adsorbent. Protein Expr. Purif. 42: 160-165.
8. Ling, T. C. (2002) Development of Rapid and Selective Method for the Direct Recovery of Intracellular Protein from Bakers' Yeast. Ph.D. Thesis. The University of Birmingham, Birmingham, UK.
9. Zhu, J., A. Lyddiatt, A. W. Pacek, and A. W. Nienow (1997) Fabrication and characterisation of agar/zircon sand composite adsorbents for protein recovery in liquid fluidised beds. pp. 103-114. In: A. W. Nienow (eds.). Bioreactor/Process Fluid Dynamics. BHR Group/Mechanical Engineering Publications, London, UK.
10. Dean, P. D. G. and D. H. Watson (1979) Protein purification using immobilized traizine dyes. J. Chromatogr. 165: 301-319.
11. Chambers, G. K. (1977) Determination of Cibacron Blue F3gA substitution in blue Sephadex and blue dextran-Sepharose. Anal. Biochem. 83: 551-556.
12. Barnfield Frej, A. K., H. J. Johansson, S. Johansson, and P. Leijon (1997) Expanded bed adsorption at production scale: Scale-up verification, process example, and sanitization of column and adsorbent. Bioprocess Biosyst. Eng. 16: 57-63.
13. Amersham Biosciences (1997) Expanded Bed Adsorption: Principles and Methods, Handbooks. pp. 14-16. Uppsala, Sweden.
14. Richardson, J. F. and W. N. Zaki (1954) Sedimentation and fluidization: Part I. Trans. Inst. Chem. Eng. 32: 35-52.
15. Nayak, D. P., S. Ponrathnam, and C. R. Rajan (2001) Macroporous copolymer matrix. IV. Expanded bed adsorption application. J. Chromatogr. A 922: 63-76.
16. Draeger, N. M. and H. A. Chase (1991) Liquid fluidized bed adsorption of protein in the presence of cells. Bioseparation 2: 67-80.
17. Chang, Y. K. and H. A. Chase (1996) Development of operating conditions for protein purification using expanded bed techniques: The effect of the degree of bed expansion on adsorption performance. Biotechnol. Bioeng. 49: 512-526.
18. Ameskamp, N., C. Priesner, J. Lehmann, and D. Lutkemeyer (1999) Pilot scale recovery of monoclonal antibodies by expanded bed ion exchange adsorption. Bioseparation 8: 169-188.
19. Thömmes J., M. Weiher, A. Karau, and M. R. Kula (1995) Hydrodynamics and performance in fluidized bed adsorption. Biotechnol. Bioeng. 48: 367-374.
20. Anspach, F. B., D. Curbelo, R. Hartmann, G. Garke, and W. D. Deckwer (1999) Expanded-bed chromatography in primary protein purification. J. Chromatogr. A 865: 129-44.
21. Tong, X. D. and Y. Sun (2002) Particle size and density distributions of two dense matrices in an expanded bed system. J. Chromatogr. A 977: 173-183.
22. Bruce, L. J. and H. A. Chase (2001) Hydrodynamics and adsorption behaviour within an expanded bed adsorption column studied using in-bed sampling. Chem. Eng. Sci. 56: 3149-3162.
23. Bascoul, A., J. P. Couderc, and H. Delmas (1993) Mouvement des particules solides en fluidisation liquide solide. Chem. Eng. J. 51: 135-150.
24. Kaufmann, M. (1997) Unstable proteins: how to subject them to chromatographic separations for purification procedures. J. Chromatogr. B 699: 347-369.