Ex vivo assessment of chemotherapy-induced apoptosis and associated molecular changes in patient tumor samples

Anticancer Research

Volumn 26, Issue 3 A, 2006, Pages 1765-1772

  Pirnia, Farzaneh ^a   Frese, Steffen ^a   Gloor, Beat ^a   Hotz, Michel A ^a   Luethi, Alexander ^a   Gugger, Mathias ^b   Betticher, Daniel C ^a   Borner, Markus M ^a,b  

^a UNIVERSITY OF BERN   (Switzerland)

^b UNIVERSITY OF BERN   (Switzerland)

Author keywords

Apoptosis; ex vivo; Non small cell lung cancer; Quantitative PCR

Indexed keywords

BENZYLOCARBONYL VALYL ALANYL ASPARTYL FLUOROMETHYL KETONE; CASPASE 3; CASPASE INHIBITOR; CISPLATIN; DOCETAXEL; MITOMYCIN C; UNCLASSIFIED DRUG;

APOPTOSIS; ARTICLE; CELL DEATH; CHEMOTHERAPY; CHROMATIN; CONTROLLED STUDY; DRUG EFFICACY; DRUG MECHANISM; ENZYME ACTIVATION; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; IN VITRO STUDY; LUNG NON SMALL CELL CANCER; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; RNA EXTRACTION; TISSUE CULTURE;

ANTINEOPLASTIC AGENTS; APOPTOSIS; CARCINOMA, NON-SMALL-CELL LUNG; CASPASE 3; CASPASES; CISPLATIN; DNA-BINDING PROTEINS; DRUG SCREENING ASSAYS, ANTITUMOR; ENZYME ACTIVATION; FEMALE; GENES, TUMOR SUPPRESSOR; HUMANS; IMMUNOHISTOCHEMISTRY; KI-67 ANTIGEN; LUNG NEOPLASMS; MALE; MITOMYCIN; NUCLEAR PROTEINS; TAXOIDS; TUMOR CELLS, CULTURED; TUMOR SUPPRESSOR PROTEINS;

EID: 33745591139     PISSN: 02507005     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (42)

1. Hoffman RM, Connors KM, Meerson-Monosov AZ, Herrera H and Price JH: A general native-state method for determination of proliferation capacity of human normal and tumor tissues in vitro. Proc Natl Acad Sci USA 86(6): 2013-2017, 1989.
2. Vescio RA, Redfern CH, Nelson TJ, Ugoretz S, Stern PH and Hoffman RM: In vivo-like drug responses of human tumors growing in three-dimensional gel-supported primary culture. Proc Natl Acad Sci USA 84(14): 5029-5033, 1987.
3. Hoffman RM: The three-dimensional question: can clinically relevant tumor drug resistance be measured in vitro? Cancer Metastasis Rev 13(2): 169-173, 1994.
4. Nakamura T, Kato Y, Fuji H, Horiuchi T, Chiba Y and Tanaka K: E-cadherin-dependent intercellular adhesion enhances chemoresistance. Int J Mol Med 12(5): 693-700, 2003.
5. Green SK, Frankel A and Kerbel RS: Adhesion-dependent multicellular drug resistance. Anticancer Drug Des 14(2): 153-168, 1999.
6. Furukawa T, Kubota T, Watanabe M, Kase S, Saikawa Y, Nishibori H et al: Increased drug resistance of cultured human cancer cell lines in three-dimensional cellular growth assay using collagen gel matrix. J Surg Oncol 49(2): 86-92, 1992.
7. Kerbel RS, St. Croix B, Florenes VA and Rak J: Induction and reversal of cell adhesion-dependent multicellular drug resistance in solid breast tumors. Hum Cell 9(4): 257-264, 1996.
8. 3H]thymidine incorporation assay. J Immunol Methods 170(2): 211-224, 1994.
9. Hamid R, Rotshteyn Y, Rabadi L, Parikh R and Bullock P: Comparison of Alamar Blue and MTT assays for high throughput screening. Toxicol In Vitro 18(5): 703-710, 2004.
10. Gloeckner H, Jonuleit T and Lemke HD: Monitoring of cell viability and cell growth in a hollow-fiber bioreactor by use of the dye Alamar Blue. J Immunol Meth 252(1-2): 131-138, 2001.
11. Byth HA, McHunu BI, Dubery IA and Bornman L: Assessment of a simple, non-toxic Alamar Blue cell survival assay to monitor tomato cell viability. Phytochem Anal 12(5): 340-346, 2001.
12. Johnstone RW, Ruefli AA and Lowe SW: Apoptosis: a link between cancer genetics and chemotherapy. Cell 108(2): 153-164, 2002.
13. Dixon SC, Soriano BJ, Lush RM, Borner MM and Figg WD: Apoptosis: its role in the development of malignancies and its potential as a novel therapeutic target. Ann Pharmacother 31(1): 76-82, 1997.
14. Borner MM, Myers CE, Sartor O, Sei Y, Toko T, Trepel JB et al: Drug-induced apoptosis is not necessarily dependent on macromolecular synthesis or proliferation in the p53-negative human prostate cancer cell line PC-3. Cancer Res 55(10): 2122-2128, 1995.
15. Pirnia F, Schneider E, Betticher DC and Borner MM: Mitomycin C induces apoptosis and caspase-8 and -9 processing through a caspase-3 and Fas-independent pathway. Cell Death Differ 9(9): 905-914, 2002.
16. Grob TJ, Novak U, Maisse C, Barcaroli D, Luthi AU, Pirnia F et al: Human delta Np73 regulates a dominant negative feedback loop for TAp73 and p53. Cell Death Differ 8(12): 1213-1223, 2001.
17. Strasser A, Harris AW, Huang DC, Krammer PH and Cory S: Bcl-2 and Fas/APO-1 regulate distinct pathways to lymphocyte apoptosis. EMBO J 14(24): 6136-6147, 1995.
18. Thornberry NA and Lazebnik Y: Caspases: enemies within. Science 281(5381): 1312-1316, 1998.
19. Melino G, De Laurenzi V and Vousden KH: p73: friend or foe in tumorigenesis. Nat Rev Cancer 2(8): 605-615, 2002.
20. Lunghi P, Costanzo A, Levrero M and Bonati A: Treatment with arsenic trioxide (ATO) and MEK1 inhibitor activates the p73-p53AIP1 apoptotic pathway in leukemia cells. Blood 104(2): 519-525, 2004.
21. Gore SD, Weng LJ and Burke PJ: Validation of flow-cytometric determination of Ki67 expression as a measure of growth factor response in acute myelogenous leukemia. Exp Hematol 21(13): 1702-1708, 1993.
22. Vescio RA, Connors KM, Bordin GM, Robb JA, Youngkin T, Umbreit JN et al: The distinction of small cell and non-small cell lung cancer by growth in native-state histoculture. Cancer Res 50(18): 6095-6099, 1990.
23. Griffon-Etienne G, Merlin JL and Marchal C: Evaluation of Taxol in head and neck squamous carcinoma multicellular tumor spheroids. Anticancer Drugs 8(1): 48-55, 1997.
24. Freeman AE and Hoffman RM: In vivo-like growth of human tumors in vitro. Proc Natl Acad Sci USA 83(8): 2694-2698, 1986.
25. Kubota T, Sasano N, Abe O, Nakao I, Kawamura E, Saito T et al: Potential of the histoculture drug-response assay to contribute to cancer patient survival. Clin Cancer Res 1(12): 1537-1543, 1995.
26. Li LN, Margolis LB and Hoffman RM: Skin toxicity determined in vitro by three-dimensional, native-state histoculture. Proc Natl Acad Sci USA 88(5): 1908-1912, 1991.
27. Vescio RA, Connors KM, Youngkin T, Bordin GM, Robb JA, Umbreit JN et al: Cancer biology for individualized therapy: correlation of growth fraction index in native-state histoculture with tumor grade and stage. Proc Natl Acad Sci USA 87(2): 691-695, 1990.
28. Furukawa T, Kubota T, Watanabe M, Kase S, Takahara T, Yamaguchi H et al: Chemosensitivity testing of clinical gastrointestinal cancers using histoculture and the MTT endpoint. Anticancer Res 12(5): 1377-1382, 1992.
29. Robbins KT, Varki NM, Storniolo AM, Hoffman H and Hoffman RM: Drug response of head and neck tumors in native-state histoculture. Arch Otolaryngol Head Neck Surg 117(1): 83-86, 1991.
30. Singh B, Li R, Xu L, Poluri A, Patel S, Shaha AR et al: Prediction of survival in patients with head and neck cancer using the histoculture drug response assay. Head Neck 24(5): 437-442, 2002.
31. Hoffman RM: To do tissue culture in two or three dimensions? That is the question. Stem Cells 11(2): 105-111, 1993.
32. Barzanti F, Zoli W, Susino MD, Ricotti L, Tesei A, Papa S et al: Simultaneous determination of apoptosis and surface antigen expression in tumor adherent cells. J Biol Regul Homeost Agents 15(4): 359-365, 2001.
33. Brouwer RE, Hoefnagel J, Borger van Der Burg B, Jedema I, Zwinderman BCH, Starrenburg IC et al: Expression of co-stimulatory and adhesion molecules and chemokine or apoptosis receptors on acute myeloid leukaemia: high CD40 and CD11a expression correlates with poor prognosis. Br J Haematol 115(2): 298-308, 2001.
34. Damiano JS, Hazlehurst LA and Dalton WS: Cell adhesion-mediated drug resistance (CAM-DR) protects the K562 chronic myelogenous leukemia cell line from apoptosis induced by BCR/ABL inhibition, cytotoxic drugs, and gamma-irradiation. Leukemia 15(8): 1232-1239, 2001.
35. Kim JB, Stein R and O'Hare MJ: Three-dimensional in vitro tissue culture models of breast cancer - a review. Breast Cancer Res Treat 85(3): 281-291, 2004.
36. Morin PJ: Drug resistance and the microenvironment: nature and nurture. Drug Resist Updat 6(4): 169-172, 2003.
37. Truong T, Sun G, Doorly M, Wang JY and Schwartz MA: Modulation of DNA damage-induced apoptosis by cell adhesion is independently mediated by p53 and c-Abl. Proc Natl Acad Sci USA 100(18): 10281-10286, 2003.
38. Kraus AC, Ferber I, Bachmann SO, Specht H, Wimmel A, Gross MW et al: In vitro chemo- and radio-resistance in small cell lung cancer correlates with cell adhesion and constitutive activation of AKT and MAP kinase pathways. Oncogene 21(57): 8683-8695, 2002.
39. Rintoul RC and Sethi T: Extracellular matrix regulation of drug resistance in small-cell lung cancer. Clin Sci (Lond) 102(4): 417-424, 2002.
40. Tanino H, Oura S, Hoffman RM, Kubota T, Furukawa T, Arimoto J et al: Acquisition of multidrug resistance in recurrent breast cancer demonstrated by the histoculture drug response assay. Anticancer Res 21(6A): 4083-4086, 2001.
41. Hoffman RM: In vitro sensitivity assays in cancer: a review, analysis, and prognosis. J Clin Lab Anal 5(2): 133-143, 1991.
42. Schrag D, Garewal HS, Burstein HJ, Samson DJ, Von Hoff DD and Somerfield MR: American Society of Clinical Oncology Technology Assessment: chemotherapy sensitivity and resistance assays. J Clin Oncol 22(17): 3631-3638, 2004.