Formation of a functional thymus initiated by a postnatal epithelial progenitor cell

Nature

Volumn 441, Issue 7096, 2006, Pages 992-996

  Bleul, Conrad C ^a   Corbeaux, Tatiana ^a   Reuter, Alexander ^a,c   Fisch, Paul ^b   Mönting, Jürgen Schulte ^b   Boehm, Thomas ^a  

^a MAX PLANCK INSTITUTE OF IMMUNOBIOLOGY AND EPIGENETICS   (Germany)

^b UNIVERSITY OF FREIBURG   (Germany)

^c UNIVERSITY OF KONSTANZ   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYMES; GENETIC ENGINEERING; IMMUNOLOGY; MORPHOLOGY;

EPITHELIAL CELLS; GENETIC DEFECTS; POSTNATAL PROGENITORS; THYMIC EPITHELIAL CELLS (TEC);

CELLS;

AUTOIMMUNE REGULATOR PROTEIN; FORKHEAD BOX N1 PROTEIN; NUCLEIC ACID BINDING PROTEIN; UNCLASSIFIED DRUG;

BIRTH RATE; CELL ORGANELLE; IMMUNE RESPONSE; MORPHOLOGY;

ADOPTIVE IMMUNOTHERAPY; AIRE GENE; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; AUTOIMMUNITY; CELL FUNCTION; CELL LINEAGE; CELL SELECTION; CELL STRUCTURE; CONTROLLED STUDY; EFFECTOR CELL; EMBRYO; EPITHELIUM CELL; FEMALE; FOXN1 GENE; GENE; GENE MUTATION; IMMUNE DEFICIENCY; IMMUNOLOGICAL TOLERANCE; IN VIVO STUDY; LYMPHOCYTE DIFFERENTIATION; MALE; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; POSTNATAL DEVELOPMENT; PRIORITY JOURNAL; REGULATORY T LYMPHOCYTE; STEM CELL; THYMUS; THYMUS DISEASE; THYMUS FUNCTION;

EID: 33745532385     PISSN: 00280836     EISSN: 14764687     Source Type: Journal    
DOI: 10.1038/nature04850     Document Type: Article

References (30)

1. Boehm, T., Bleul, C. C. & Schorpp, M. Genetic dissection of thymus development in mouse and zebrafish. Immunol. Rev. 195, 15-27 (2003).
2. Anderson, G. & Jenkinson, E. J. Lymphostromal interactions in thymic development and function. Nature Rev. Immunol. 1, 31-40 (2001).
3. Gray, D. H. et al. Controlling the thymic microenvironment. Curr. Opin. Immunol. 17, 137-143 (2005).
4. Nehls, M. et al. Two genetically separable steps in the differentiation of thymic epithelium. Science 272, 886-889 (1996).
5. Anderson, M. S. et al. Projection of an immunological self shadow within the thymus by the aire protein. Science 298, 1395-1401 (2002); published online 10 October 2002 (doi: 10.1126/science.1075958).
6. Hogquist, K. A. & Bevan, M. J. The nature of the peptide/MHC ligand involved in positive selection. Semin. Immunol. 8, 63-68 (1996).
7. Kyewski, B., & Derbinski, J. Self-representation in the thymus: an extended view. Nature Rev. Immunol. 4, 688-698 (2004).
8. Blackburn, C. C. et al. One for all and all for one: thymic epithelial stem cells and regeneration. Trends Immunol. 23, 391-395 (2002).
9. Nehls, M., Pfeifer, D., Schorpp, M., Hedrich, H. & Boehm, T. New member of the winged-helix protein family disrupted in mouse and rat nude mutations. Nature 372, 103-107 (1994).
10. Blackburn, C. C. et al. The nu gene acts cell-autonomously and is required for differentiation of thymic epithelial progenitors. Proc. Natl Acad. Sci. USA 93, 5742-5746 (1996).
11. Cunningham-Rundles, C. & Ponda, P. P. Molecular defects in T- and B-cell primary immunodeficiency diseases. Nature Rev. Immunol. 5, 880-892 (2005).
12. Boehm, T., Scheu, S., Pfeffer, K. & Bleul, C. C. Thymic medullary epithelial cell differentiation, thymocyte emigration, and the control of autoimmunity require lympho-epithelial cross talk via LTbetaR. J. Exp. Med. 198, 757-769 (2003).
13. Akiyama, T. et al. Dependence of self-tolerance on TRAF6-directed development of thymic stroma. Science 308, 248-251 (2005); published online 10 February 2005 (doi: 10.1126/science.1105677).
14. Bennett, A. R. et al. Identification and characterization of thymic epithelial progenitor cells. Immunity 16, 803-814 (2002).
15. Gill, J., Malin, M., Hollander, G. A. & Boyd, R. Generation of a complete thymic microenvironment by MTS24(+) thymic epithelial cells. Nature Immunol. 3, 635-642 (2002); advance online publication 17 June 2002 (doi:10.1038/ni812).
16. Srinivas, S. et al. Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus. BMC Dev. Biol. 1, 4 (2001); published online 27 March 2001 (doi: 10.1186/1471-213x-1-4).
17. Li, M. et al. Skin abnormalities generated by temporally controlled RXRalpha mutations in mouse epidermis. Nature 407, 633-636 (2000).
18. Vassar, R., Rosenberg, M., Ross, S., Tyner, A. & Fuchs, E. Tissue-specific and differentiation-specific expression of a human K14 keratin gene in transgenic mice. Proc. Natl Acad. Sci. USA 86, 1563-1567 (1989).
19. Rodewald, H. R., Paul, S., Haller, C., Bluethmann, H. & Blum, C. Thymus medulla consisting of epithelial islets each derived from a single progenitor. Nature 414, 763-768 (2001).
20. Soriano, P. Generalized lacZ expression with the ROSA26 Cre reporter strain. Nature Genet. 21, 70-71 (1999).
21. Huth, M., Schlake, T. & Boehm, T. Transposon-induced splicing defect in the rat nude gene. Immunogenetics 45, 282-283 (1997).
22. Adriani, M. et al. Ancestral founder mutation of the nude (FOXN1) gene in congenital severe combined immunodeficiency associated with alopecia in southern Italy population. Ann. Hum. Genet. 68, 265-268 (2004).
23. Frank, J. et al. Exposing the human nude phenotype. Nature 398, 473-474 (1999).
24. Pignata, C. et al. Human equivalent of the mouse Nude/SCID phenotype: long-term evaluation of immunologic reconstitution after bone marrow transplantation. Blood 97, 880-885 (2001).
25. Rossi, S. W., Jenkinson, W. E., Anderson, G. & Jenkinson, E. J. Clonal analysis reveals a common progenitor for thymic cortical and medullary epithelium. Nature doi:10.1038/nature04813 (this issue).
26. Schwenk, F., Baron, U., & Rajewsky, K. A cre-transgenic mouse strain for the ubiquitous deletion of loxP-flanked gene segments including deletion in germ cells. Nucleic Acids Res. 23, 5080-5081 (1995).
27. Nehls, M., Messerle, M., Sirulnik, A., Smith, A. J. & Boehm, T. Two large insert vectors, lambda PS and lambda KO, facilitate rapid mapping and targeted disruption of mammalian genes. Biotechniques 17, 770-775 (1994).
28. Nehls, M., Pfeifer, D., Micklem, G., Schmoor, C. & Boehm, T. The sequence complexity of exons trapped from the mouse genome. Curr. Biol. 4, 983-989 (1994).
29. te Riele, H., Maandag, E. R., Clarke, A., Hooper, M. & Berns, A. Consecutive inactivation of both alleles of the pim-1 proto-oncogene by homologous recombination in embryonic stem cells. Nature 348, 649-651 (1990).
30. Schorpp, M., Hofmann, M., Dear, T. N. & Boehm, T. Characterization of mouse and human nude genes. Immunogenetics 46, 509-515 (1997).