Alternative method of rapid drying vascular specimens for scanning electron microscopy

Journal of Endovascular Therapy

Volumn 10, Issue 2, 2003, Pages 285-287

  Slížvá, Dáša ^a,b   Krs, Otakar ^a   Pospíšilová, Blanka ^a  

^a UNIVERSITY HOSPITAL HRADEC KRALOVE   (Czech Republic)

^b CHARLES UNIVERSITY   (Czech Republic)

Author keywords

Blood vessels; Desiccation; Endothelium; Hexamethyldisilazane; Morphology; Neointima; Scanning electron microscopy; Stent

Indexed keywords

ALCOHOL; DESICCANT; HEXAMETHYLDISILAZANE; STAINLESS STEEL; UNCLASSIFIED DRUG;

ANIMAL TISSUE; ARTICLE; BLOOD VESSEL WALL; CONTROLLED STUDY; COST; DEHYDRATION; DESICCATION; DIAGNOSIS, MEASUREMENT AND ANALYSIS; ENDOTHELIUM; HISTOPATHOLOGY; INTIMA; NONHUMAN; PRIORITY JOURNAL; QUALITY CONTROL; SCANNING ELECTRON MICROSCOPY; STENT; TISSUE FIXATION; VASCULAR TISSUE;

ANIMALS; BLOOD VESSELS; DOGS; FIXATIVES; MICROSCOPY, ELECTRON, SCANNING; ORGANOSILICON COMPOUNDS; TIME FACTORS; TISSUE FIXATION;

EID: 0041731700     PISSN: 15266028     EISSN: None     Source Type: Journal    
DOI: 10.1583/1545-1550(2003)010<0285:AMORDV>2.0.CO;2     Document Type: Article

References (15)

1. Kaska M, Pospisilova B, Slizova D. Pathomorphological changes in microcirculation of pancreas during experimental acute pancreatitis. Hepatogastroenterology. 2000;47:1570-1574.
2. Ferko A, Paral J, Raupach J, et al. Autologous vein stent-graft: feasibility study. J Vasc Interv Radiol. 2000;11:111-114.
3. Nation JL. A new method using hexamethyldisilazane for preparation of soft insect tissues for scanning electron microscopy. Stain Technol. 1983;58:347-351.
4. Foliguet B, Vicari F, Guedenet JC, et al. Scanning electron microscopic study of Campylobacter pylori and associated gastroduodenal lesions [in French], Gastroenterol Clin Biol. 1989;13:65B-70B.
5. Kolb BA, Najmabadi S, Paulson RJ, Ultrastructural characteristics of the luteal phase endometrium in patients undergoing controlled ovarian hyperstimulation. Fertil Steril. 1997;67: 625-630.
6. Hirst RA, Sikand KS, Rutman A, et al. Relative roles of pneumolysin and hydrogen peroxide from Streptococcus pneumoniae in inhibition of ependymal ciliary beat frequency. Infect Immun. 2000;68:1557-1562.
7. Heegaard S, Jensen OA, Prause JU. Hexamethyldisilazane in preparation of retinal tissue for scanning electron microscopy. Ophthalmic Res. 1986;18:203-208.
8. Forge A, Nevill G, Zajic G, et al. Scanning electron microscopy of the mammalian organ of Corti: assessment of preparative procedures. Scanning Microsc. 1992;6:521-534.
9. Weryha G, Marchal L, Leclere J, et al. Scanning electron microscopy (SEM) of the human thyroid gland: a technical improvement for follicular cell observation. J Submicrosc Cytol Pathol. 1990;22:459-464.
10. Maeda N, Hagihara H, Nakata Y, et al. Aortic wall damage in mice unable to synthesize ascorbic acid. Proc Natl Acad Sci USA. 2000;97: 841-846.
11. Kuhnel T, Koveker G, Muller GH. Drying with hexamethyldisilazane - a time-saving alternative to the "critical point" method [in German]. Handchir Mikrochir Plast Chir. 1989;21:164-165.
12. Wong PC, Crain EJ, Knabb RM, et al. Nonpeptide factor Xa inhibitors II. Antithrombotic evaluation in a rabbit model of electrically induced carotid artery thrombosis. J Pharmacol Exp Ther. 2000;295:212-218.
13. Macdonald RL, Mojtahedi S, Johns L, et al. Randomized comparison of Guglielmi detachable coils and cellulose acetate polymer for treatment of aneurysms in dogs. Stroke. 1998; 29:478-486.
14. Bray DF, Bagu J, Koegler P. Comparison of hexamethyldisilazane (HMDS), Peldri II, and critical-point drying methods for scanning electron microscopy of biological specimens. Microsc Res Tech. 1993;26:489-495.
15. Krizmanich WJ, Lee RM. Scanning electron microscopy of vascular smooth muscle cells from spontaneously hypertensive rats. Scanning Microsc. 1993;7:129-135.