Osteogenic differentiation within intact human embryoid bodies result in a marked increase in osteocalcin secretion after 12 days of in vitro culture, and formation of morphologically distinct nodule-like structures

Tissue and Cell

Volumn 37, Issue 4, 2005, Pages 325-334

  Cao, Tong ^a   Heng, Boon Chin ^a   Ye, Chao Peng ^a   Liu, Hua ^a   Toh, Wei Seong ^a   Robson, Paul ^b   Li, Pin ^b   Hong, Yun Han ^c   Stanton, Lawrence Walter ^b  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^b GENOME INSTITUTE OF SINGAPORE   (Singapore)

^c NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

Differentiation; Embryonic; Human; Osteocalcin; Osteogenic; Stem cells

Indexed keywords

ASCORBIC ACID; CHEMICAL COMPOUND; CYTOKINE; DEXAMETHASONE; GLYCEROL 2 PHOSPHATE; GROWTH FACTOR; OSTEOCALCIN;

ARTICLE; BONE DEVELOPMENT; CELL DIFFERENTIATION; CELL GROWTH; CELL MIGRATION; CELL STRUCTURE; CONTROLLED STUDY; CULTURE MEDIUM; EMBRYONIC STEM CELL; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; IN VITRO STUDY; MESENCHYMAL STEM CELL; OSTEOBLAST; PROTEIN SECRETION; QUANTITATIVE ASSAY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

EID: 21644442456     PISSN: 00408166     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.tice.2005.03.008     Document Type: Article

References (32)

1. J.A. Andrades B. Han J. Becerra N. Sorgente F.L. Hall M.E. Nimni A recombinant human TGF-beta1 fusion protein with collagen-binding domain promotes migration, growth, and differentiation of bone marrow mesenchymal cells Exp. Cell Res. 250 2 1999 485-498
2. S. Bourne J.M. Polak S.P. Hughes L.D. Buttery Osteogenic differentiation of mouse embryonic stem cells: Differential gene expression analysis by cDNA microarray and purification of osteoblasts by cadherin-11 magnetically activated cell sorting Tissue Eng. 10 5-6 2004 796-806
3. A.L. Bronckers S. Gay R.D. Finkelman W.T. Butler Developmental appearance of Gla proteins (osteocalcin) and alkaline phosphatase in tooth germs and bones of the rat Bone Miner. 2 5 1987 361-373
4. L.D. Buttery S. Bourne J.D. Xynos H. Wood F.J. Hughes S.P. Hughes V. Episkopou J.M. Polak Differentiation of osteoblasts and in vitro bone formation from murine embryonic stem cells Tissue Eng. 7 1 2001 89-99
5. G.R. Chaudhry D. Yao A. Smith A. Hussain Osteogenic cells derived from embryonic stem cells produced bone nodules in three-dimensional scaffolds J. Biomed. Biotechnol. 2004 4 2004 203-210
6. H. Cheng W. Jiang F.M. Phillips R.C. Haydon Y. Peng L. Zhou H.H. Luu N. An B. Breyer P. Vanichakarn J.P. Szatkowski J.Y. Park T.C. He Osteogenic activity of the fourteen types of human bone morphogenetic proteins (BMPs) J. Bone Joint Surg. Am. 85-A 8 2003 1544-1552
7. H. Chiba N. Sawada M. Oyamada T. Kojima S. Nomura S. Ishii M. Mori Relationship between the expression of the gap junction protein and osteoblast phenotype in a human osteoblastic cell line during cell proliferation Cell Struct. Funct. 18 6 1993 419-426
8. J.E. Dennis J.P. Carbillet A.I. Caplan P. Charbord The STRO-1+ marrow cell population is multipotential Cells Tissues Organs 170 2-3 2002 73-82
9. G. D'Ippolito P.C. Schiller C. Ricordi B.A. Roos G.A. Howard Age-related osteogenic potential of mesenchymal stromal stem cells from human vertebral bone marrow J. Bone Miner. Res. 14 7 1999 1115-1122
10. H.J. Donahue Z. Li Z. Zhou C.E. Yellowley Differentiation of human fetal osteoblastic cells and gap junctional intercellular communication Am. J. Physiol. Cell Physiol. 278 2 2000 C315-C322
11. M. Drukker N. Benvenisty The immunogenicity of human embryonic stem-derived cells Trends Biotechnol. 22 3 2004 136-141
12. T. Fujita R. Fukuyama N. Izumo T. Hirai T. Meguro H. Nakamuta M. Koida Transactivation of core binding factor alpha1 as a basic mechanism to trigger parathyroid hormone-induced osteogenesis Jpn. J. Pharmacol. 86 4 2001 405-416
13. B.K. Hall T. Miyake Divide, accumulate, differentiate: Cell condensation in skeletal development revisited Int. J. Dev. Biol. 39 6 1995 881-893
14. B.C. Heng T. Cao L.W. Stanton P. Robson B. Olsen Strategies for directing the differentiation of stem cells into the osteogenic lineage in vitro J. Bone Miner. Res. 19 9 2004 1379-1394
15. W.S. Hwang Y.J. Ryu J.H. Park E.S. Park E.G. Lee J.M. Koo H.Y. Jeon B.C. Lee S.K. Kang S.J. Kim C. Ahn J.H. Hwang K.Y. Park J.B. Cibelli S.Y. Moon Evidence of a pluripotent human embryonic stem cell line derived from a cloned blastocyst Science 303 5664 2004 1669-1674
16. D. Kedra M. Peyrard I. Fransson J.E. Collins I. Dunham B.A. Roe J.P. Dumanski Characterization of a second human clathrin heavy chain polypeptide gene (CLH-22) from chromosome 22q11 Hum. Mol. Genet. 5 5 1996 625-631
17. R. Minkoff V.R. Rundus S.B. Parker E.L. Hertzberg J.G. Laing E.C. Beyer Gap junction proteins exhibit early and specific expression during intramembranous bone formation in the developing chick mandible Anat. Embryol. (Berl.) 190 3 1994 231-241
18. NIH backgrounder on stem cells (2003) accessed on 8th November 2004: http://www.nih.gov/news/backgrounders/stemcellbackgrounder.htm
19. W.R. Otto J. Rao Tomorrow's skeleton staff: Mesenchymal stem cells and the repair of bone and cartilage Cell Prolif. 37 1 2004 97-110
20. M.Z. Ratajczak M. Kucia M. Majka R. Reca J. Ratajczak Heterogeneous populations of bone marrow stem cells - are we spotting on the same cells from the different angles? Folia Histochem. Cytobiol. 42 3 2004 139-146
21. B.E. Reubinoff M.F. Pera C.Y. Fong A. Trounson A. Bongso Embryonic stem cell lines from human blastocysts: Somatic differentiation in vitro Nat. Biotechnol. 18 4 2000 399-404
22. G.A. Rodan S.B. Rodan Hormone-adenylate cyclase coupling in osteosarcoma clonal cell lines Adv. Cyclic Nucleotide Protein Phosphorylation Res. 17 1984 127-134
23. P.J. Simmons B. Torok-Storb Identification of stromal cell precursors in human bone marrow by a novel monoclonal antibody, STRO-1 Blood 78 1 1991 55-62
24. V. Sottile A. Thomson J. McWhir In vitro osteogenic differentiation of human ES cells Cloning Stem Cells 5 2 2003 149-155
25. M. Sugiyama T. Kodama K. Konishi K. Abe S. Asami S. Oikawa Compactin and simvastatin, but not pravastatin, induce bone morphogenetic protein-2 in human osteosarcoma cells Biochem. Biophys. Res. Commun. 271 3 2000 688-692
26. J.A. Thomson J. Itskovitz-Eldor S.S. Shapiro M.A. Waknitz J.J. Swiergiel V.S. Marshall J.M. Jones Embryonic stem cell lines derived from human blastocysts Science 282 5391 1998 1145-1147
27. A. Trounson Human embryonic stem cells: Mother of all cell and tissue types Reprod. Biomed. Online 4 Suppl. 1 2002 58-63
28. M. Tzukerman T. Rosenberg Y. Ravel I. Reiter R. Coleman K. Skorecki An experimental platform for studying growth and invasiveness of tumor cells within teratomas derived from human embryonic stem cells Proc. Natl. Acad. Sci. USA 100 23 2003 13507-13512
29. J.P. van Leeuwen M. van Driel G.J. van den Bemd H.A. Pols Vitamin D control of osteoblast function and bone extracellular matrix mineralization Crit. Rev. Eukaryot. Gene Expr. 11 1-3 2001 199-226
30. M.A. Vander Molen C.T. Rubin K.J. McLeod L.K. McCauley H.J. Donahue Gap junctional intercellular communication contributes to hormonal responsiveness in osteoblastic networks J. Biol. Chem. 271 21 1996 12165-12171
31. C.M. Verfaillie Adult stem cells: Assessing the case for pluripotency Trends Cell Biol. 12 11 2002 502-508
32. N.I. zur Nieden G. Kempka H.J. Ahr In vitro differentiation of embryonic stem cells into mineralized osteoblasts Differentiation 71 1 2003 18-27