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This study used 2-photon microscopy of intact lymph nodes to define the kinetics and micro-anatomical requirements for antigen-specific B cell-T cell interactions. Antigen-engaged B cells within 100 μm of the follicle edge were observed to migrate along relatively straight paths to the edge of the follicular boundary in a CCR7 chemokine receptor-determined fashion. A gradient of CCL21 extending into the follicle over the same range of 100 μm was detected. The antigen-engaged B cells formed long-lasting and highly motile conjugates with antigen-specific helper T cells. These findings provide the first indication of lymphocyte chemotaxis in vivo, and define the complex cellular dynamics of T cell-dependent antibody responses.
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Okada T., Miller M.J., Parker I., Krummel M.F., Neighbors M., Hartley S.B., O'Garra A., Cahalan M.D., and Cyster J.G. Antigen-engaged B cells undergo chemotaxis toward the T zone and form motile conjugates with helper T cells. PLoS Biol 3 (2005) e150. This study used 2-photon microscopy of intact lymph nodes to define the kinetics and micro-anatomical requirements for antigen-specific B cell-T cell interactions. Antigen-engaged B cells within 100 μm of the follicle edge were observed to migrate along relatively straight paths to the edge of the follicular boundary in a CCR7 chemokine receptor-determined fashion. A gradient of CCL21 extending into the follicle over the same range of 100 μm was detected. The antigen-engaged B cells formed long-lasting and highly motile conjugates with antigen-specific helper T cells. These findings provide the first indication of lymphocyte chemotaxis in vivo, and define the complex cellular dynamics of T cell-dependent antibody responses.
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This study combined calcium imaging with cell tracking in a thymus slice preparation to demonstrate that a rise in cytosolic calcium level is sufficient to cause motility arrest and prolonged interaction with stromal cells during positive selection.
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Bhakta N.R., Oh D.Y., and Lewis R.S. Calcium oscillations regulate thymocyte motility during positive selection in the three-dimensional thymic environment. Nat Immunol 6 (2005) 143-151. This study combined calcium imaging with cell tracking in a thymus slice preparation to demonstrate that a rise in cytosolic calcium level is sufficient to cause motility arrest and prolonged interaction with stromal cells during positive selection.
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This study demonstrated directional migration of a subset of thymocytes from the cortex into the medulla during positive selection in the thymus.
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Visualizing regulatory T cell control of autoimmune responses in nonobese diabetic mice
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