Early detection of bacterium-induced basal resistance in tobacco leaves with diaminobenzidine and dichlorofluorescein diacetate

Journal of Phytopathology

Volumn 153, Issue 10, 2005, Pages 596-607

  Bozso Z ^a   Ott, P G ^a   Szatmari, A ^a   Czelleng, A ^a   Varga, G ^a   Besenyei, E ^a   Sardi E ^a,b   Banyai E ^a,b   Klement, Z ^a  

^a INSTITUTE OF EXPERIMENTAL MEDICINE   (Hungary)

^b Institute of Entrepreneurship and Innovation   (Hungary)

Author keywords

Basal resistance; Diaminobenzidine; General recognition mechanism; hrp mutants; Hypersensitive response; Innate immunity; Pseudomonas syringae; Tobacco

Indexed keywords

BACTERIUM; DEFENSE MECHANISM; ENZYME ACTIVITY; FECAL COLIFORM; GENE EXPRESSION; PATHOGEN; PHYSIOLOGY; VIRULENCE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NICOTIANA OBTUSIFOLIA; NICOTIANA TABACUM; PSEUDOMONAS FLUORESCENS; PSEUDOMONAS SYRINGAE; PSEUDOMONAS SYRINGAE PV. SYRINGAE;

EID: 33745298446     PISSN: 09311785     EISSN: 14390434     Source Type: Journal    
DOI: 10.1111/j.1439-0434.2005.01026.x     Document Type: Article

References (41)

1. Alfano JR Collmer A 1997) The type III (Hrp) secretion pathway of plant pathogenic bacteria: trafficking harpins, Avr proteins, and death. J Bacteriol 179 : 5655 5662.
2. Alfano JR Kim H-S Delenay TP Collmer A 1997) Evidence that the Pseudomonas syringae pv. syringae hrp-linked hrmA gene encodes an Avr-like protein that acts in an hrp-dependent manner within tobacco cells. Mol Plant Microbe Interact 10 : 580 588.
3. Allan AC Fluhr R 1997) Two distinct sources of elicited reactive oxygen species in tobacco epidermal cells. Plant Cell 9 : 1559 1572.
4. Alvarez ME Pennell RI Meijer PJ Ishikawa A Dixon RA Lamb C 1998) Reactive oxygen intermediates mediate a systemic signal network in the establishment of plant immunity. Cell 92 : 773 784.
5. Baker CJ Orlandi EW 1995) Active oxygen in plant pathogenesis. Ann Rev Phytopathol 33 : 299 321.
6. Bestwick CS Bennett MH Mansfield JW 1995) Hrp mutant of Pseudomonas syringae pv. phaseolicola induces cell wall alterations but not membrane damage leading to the HR in lettuce (Lactuca sativa). Plant Physiol 108 : 503 516.
7. Bestwick CS Brown IR Mansfield JW 1998) Localized changes in peroxidase activity accompany hydrogen peroxide generation during the development of a nonhost hypersensitive reaction in Lactuca sativa L. Plant Physiol 118 : 1067 1078.
8. Bolwell GP Wojtaszek P 1997) Mechanisms for the generation of reactive oxygen species in plant defence - a broad perspective. Physiol Mol Plant Pathol 51 : 347 366.
9. Bozsó Z Ott PG Kecskés ML Klement Z 1999) Effect of heat and cycloheximide treatment of tobacco on the ability of Pseudomonas syringae pv. syringae 61 hrp/hrmA mutants to cause HR. Physiol Mol Plant Pathol 55 : 215 223.
10. Brown IR Trethowan J Kerry M Mansfield J Bolwell GP 1998) Localization of components of the oxidative cross-linking of glycoproteins and of callose in papillae formed during the interactions between non-pathogenic strains of Xanthomonas campestris and French bean mesophyll cells. Plant J 15 : 333 343.
11. Burgyán J Klement Z 1979) Early induced selective inhibition of incompatible bacteria in tobacco plants. Phytopathol Mediterr 18 : 153 161.
12. Costet L Cordelier S Dorey S Baillieul F Fritig B Kauffmann S 1999) Relationship between localized acquired resistance (LAR) and the hypersensitive response (HR): HR is necessary for LAR to occur and salicylic acid is not sufficient to trigger LAR. Mol Plant Microbe Interact 12 : 655 662.
13. Felix G Duran JD Volko S Boller T 1999) Plants have a sensitive perception system for the most conserved domain of bacterial flagellin. Plant J 18 : 256 276.
14. Frederick SE. The cytochemistry of diaminobenzidine. In: Kevin CV ( ed. CRC Handbook of Plant Cytochemistry, Vol. 1. Boca Raton, FL 33487, USA, CRC Press, 1987, pp. 3 23.
15. Gomez-Gomez L Boller T 2002) Flagellin perception: a paradigm for innate immunity. Trends Plant Sci 7 : 251 256.
16. Hammond-Kosack KE Jones JDG 1996) Resistance gene-dependent plant defense responses. Plant Cell 8 : 1773 1791.
17. Hauck P Thilmony R He SY 2003) A Pseudomonas syringae type III effector suppresses cell wall-based extracellular defense in susceptible Arabidopsis plants. Proc Natl Acad Sci U S A 100 : 8577 8582.
18. Hoekema A Hirsch PR Hooykaas PJJ Schilperoort RA. ( 1983) A binary plant vector strategy based on separation of vir-region and T-region of the Agrobacterium tumefaciens ti-plasmid. Nature 303 : 179 180.
19. Huang HC Schuurink R Denny TP et al. ( 1988) Molecular cloning of Pseudomonas syringae pv syringae gene cluster that enables Pseudomonas fluorescens to elicit the hypersensitive response in tobacco. J Bacteriol 170 : 4748 4756.
20. Jakobek JL Lindgren PB 1993) Generalized induction of defense responses in bean is not correlated with the induction of the hypersensitive reaction. Plant Cell 5 : 49 56.
21. Kimpel JA Key JL 1985) Heat shock in plants. Trends Biochem Sci 10 : 353 357.
22. King EO Ward MK Raney DE 1954) Two simple media for the demonstration of pyocyanine and fluorescein. J Lab Med 22 : 301 307.
23. Klement Z 1963) Rapid detection of the pathogenicity of phytophatogenic pseudomonads. Nature 199 : 299 300.
24. Klement Z. Generally used pathophysiological methods. In: Klement Z Rudolph K Sands DC ( eds Methods in Phytobacteriology. Budapest, Hungary, Akadémiai Kiadó, 1990, pp. 96 121.
25. Klement Z Bozsó Z Ott PG Kecskés ML Rudolp K 1999) Symptomless resistant response instead of the hypersensitive reaction in tobacco leaves after infiltration of heterologous pathovars of Pseudomonas syringae. J Phytopathol 147 : 467 475.
26. Klement Z Bozsó Z Kecskés ML Besenyei E Czelleng A Ott PG. ( 2003) Local early induced resistance of plants as the first line of defence against bacteria. Pest Manag Sci 59 : 465 474.
27. Lovrekovich L Farkas GL 1965) Induced protection against wildfire disease in tobacco leaves treated with heat-killed bacteria. Nature 205 : 823 824.
28. Lu H Higgins VJ 1998) Measurement of active oxygen species generated in planta in response to elicitor AVR9 of Cladosporium fulvum. Physiol Mol Plant Pathol 52 : 35 51.
29. Mazzucchi U Bazzi C Pupillo P 1979) The inhibition of susceptible and hypersensitive reactions by protein-lipopolysaccharide complexes from phytopathogenic pseudomonads: relationship to polysaccharide antigenic determinants. Physiol Plant Pathol 14 : 19 30.
30. Meier MB Shaw N Slusarenko AJ 1993) Spatial and temporal accumulation of defense gene transcripts in bean (Phaseolus vulgaris) leaves in relation to bacteria-induced hypersensitive cell death. Mol Plant Microbe Interact 6 : 453 466.
31. Newman MA Daniels MJ Dow JM 1995) Lipopolysaccharide from Xanthomonas campestris induces defense-related gene expression in Brassica campestris. Mol Plant Microbe Interact 8 : 778 780.
32. Newman MA Daniels MJ Dow JM 1997) The activity of lipid A and core components of bacterial lipopolysaccharides in the prevention of the hypersensitive response in pepper. Mol Plant Microbe Interact 10 : 926 928.
33. Olson PD Varner JE 1993) Hydrogen peroxide and lignification. Plant J 4 : 887 892.
34. Ott PG Szabó L Balázs E Klement Z 1997) Submicroscopic evidence of bacterially induced resistance in tobacco leaves. Acta Phytopathol Entomol Hung 32 : 265 280.
35. Papadakis AK Roubelakis-Angelakis KA 1999) The generation of active oxygen species differs in tobacco and grapevine mesophyll protoplasts. Plant Physiol 121 : 197 205.
36. Sequeira L 1983) Mechanisms of induced resistance in plants. Ann Rev Microbiol 37 : 51 79.
37. de Torres M Sanchez P Fernandez-Delmond I Grant M 2003) Expression profiling of the host response to bacterial infection: the transition from basal to induced defence responses in RPM1-mediated resistance. Plant J 33 : 665 676.
38. Varet A Parker J Tornero P et al. ( 2002) NHL25 and NHL3, two NDR1/HIN1-1ike genes in Arabidopsis thaliana with potential role(s) in plant defense. Mol Plant Microbe Interact 15 : 608 616.
39. Veena ? Jiang H Doerge RW Gelvin SB 2003) Transfer of T-DNA and Vir proteins to plant cells by Agrobacterium tumefaciens induces expression of host genes involved in mediating transformation and suppresses host defense gene expression. Plant J 35 : 219 236.
40. Woodcock DM Crowther PJ Doherty J et al. ( 1989) Quantitative evaluation of Escherichia coli host strains for tolerance to cytosine methylation in plasmid and phage recombinants. Nucleic Acids Res 17 : 3469 3478.
41. Xiao Y Lu Y Heu S Hutcheson SW 1992) Organization and environmental regulation of the Pseudomonas syringae pv. syringae 61 hrp cluster. J Bacteriol 174 : 1734 1741.