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Using a sophisticated approach that encompasses biochemical and genetic methods, the authors describe a biological function for the MKK4-MPK6 cascade in a pathogen response that regulates ethylene biosynthesis. They found that two ACS molecules act as substrates of MPK6, which is activated by MKK4. Phosphorylation by MPK6 leads to the accumulation of ACS protein and to elevated levels of ACS activity in vivo, consequently increasing ethylene production. This represents the first evidence of a direct plant MAPK phosphorylation target, and demonstrates that ethylene is biosynthesized in response to stress signaling.
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Liu Y., and Zhang S. Phosphorylation of 1-aminocyclopropane-1-carboxylic acid synthase by MPK6, a stress-responsive mitogen-activated protein kinase, induces ethylene biosynthesis in Arabidopsis. Plant Cell 16 (2004) 3386-3399. Using a sophisticated approach that encompasses biochemical and genetic methods, the authors describe a biological function for the MKK4-MPK6 cascade in a pathogen response that regulates ethylene biosynthesis. They found that two ACS molecules act as substrates of MPK6, which is activated by MKK4. Phosphorylation by MPK6 leads to the accumulation of ACS protein and to elevated levels of ACS activity in vivo, consequently increasing ethylene production. This represents the first evidence of a direct plant MAPK phosphorylation target, and demonstrates that ethylene is biosynthesized in response to stress signaling.
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Xiong L., and Yang Y. Disease resistance and abiotic stress tolerance in rice are inversely modulated by an abscisic acid-inducible mitogen-activated protein kinase. Plant Cell 15 (2003) 745-759
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Moon H., Lee B., Choi G., Shin D., Prasad D.T., Lee O., Kwak S.S., Kim D.H., Nam J., Bahk J., et al. NDP kinase 2 interacts with two oxidative stress-activated MAPKs to regulate cellular redox state and enhances multiple stress tolerance in transgenic plants. Proc Natl Acad Sci USA 100 (2003) 358-363
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Transgenic plants expressing epitope-tagged Mla variants are used to describe the effects of the rar1 (required for Mla resistance1) mutation upon the abundance of the barley powdery mildew resistance R proteins MLA1 and MLA6. This analysis of rar1 mutants led the authors to propose that an effective resistance response requires a threshold level of R protein. In addition, the authors demonstrate that MLA protein stability decreased under high-temperature conditions. Transgene transcription levels and the amounts of RAR1 and SGT1 (SUPPRESSOR OF THE G2 ALLELE OF skp1-4) proteins remained unchanged during stress treatment.
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