Cloning and expression of a Melanocarpus albomyces steryl esterase gene in Pichia pastoris and Trichoderma reesei

Biotechnology and Bioengineering

Volumn 94, Issue 3, 2006, Pages 407-415

  Kontkanen, Hanna ^a   Reinikainen, Tapani ^b   Saloheimo, Markku ^a  

^a VTT TECHNICAL RESEARCH CENTRE OF FINLAND   (Finland)

^b National Bureau of Investigation Finland   (Finland)

Author keywords

Heterologous expression; Lipase; Melanocarpus albomyces; Pichia pastoris; Steryl esterase; Trichoderma reesei

Indexed keywords

AMINO ACIDS; CLONING; FUNGI; GENES; PROTEINS; TISSUE CULTURE;

GENE ENCODING; HETEROLOGOUS EXPRESSION; LIPASE; MATURE PROTEIN; MELANOCARPUS ALBOMYCES; PICHIA PASTORIS; STERYL ESTERASE; TRICHODERMA REESEI;

ENZYME KINETICS;

CARBOXYLESTERASE; ESTERASE; STERYL ESTERASE; TRIACYLGLYCEROL LIPASE; TRITON X 100; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; FUNGUS; GENE; GENE EXPRESSION; INTRON; MOLECULAR CLONING; MYCELIUM; NONHUMAN; NUCLEOTIDE SEQUENCE; PICHIA PASTORIS; STE1 GENE; TRICHODERMA REESEI;

AMINO ACID SEQUENCE; CLONING, MOLECULAR; CYTOPLASM; ESTERASES; FUNGAL PROTEINS; GENE EXPRESSION; MOLECULAR SEQUENCE DATA; PICHIA; PROTEIN PRECURSORS; PROTEIN SORTING SIGNALS; RECOMBINANT PROTEINS; TRICHODERMA;

FUNGI; HYPOCREA JECORINA; MELANOCARPUS ALBOMYCES; PICHIA PASTORIS;

EID: 33745196238     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.20686     Document Type: Article

References (42)

1. Akoh CC, Lee G-C, Liaw Y-C, Huang T-H, Shaw J-F. 2004. GDSL family of serine esterases/lipases. Prog Lipid Res 43:534-552.
2. Allain CC, Poon LS, Chan CSG, Richmond W, Fu PC. 1974. Enzymatic determination of total serum cholesterol. Clin Chem 20:470-475.
3. Antczak T, Patura J, Szczesna-Antczak M, Hiler D, Bielecki S. 2004. Sugar ester synthesis by a mycelium-bound Mucor circinelloides lipase in a micro-reactor equipped with water activity sensor. J Mol Cat B: Enz 29:155-161.
4. Brady L, Brozozowski AM, Derewenda ZS, Dodson E, Dodson G, Tolley S, Turkenburg JP, Christiansen L, Huge-Jensen B, Norskov L, Thim L, Menge U. 1990. A serine protease triad forms the catalytic centre of a triacylglycerol lipase. Nature 343:767-770.
5. Brocca S, Schmidt-Dannert C, Lotti M, Alberghina L, Schmid RD. 1998. Design, total synthesis and functional overexpression of the Candida rugosa lip 1 gene coding for a major industrial lipase. Protein Sci 7:1415-1422.
6. Brush TS, Chapman R, Kurzman R, Williams DP. 1999. Purification and characterization of extracellular lipases from Ophiostoma piliferum. Bioorg Med Chem 7:2131-2138.
7. Calero-Rueda O, Plou FJ, Ballesteros A, Martínez AT, Martínez MJ. 2002. Production, isolation and characterization of a sterol esterase from Ophiostoma piceae. Biochim Biophys Acta 1599:28-35.
8. Catoni E, Schmidt-Dannert C, Brocca S, Schmid RD. 1997. Overexpression of lipase A and B of Geotrichum candidum in Pichia pastoris: High-level production and some properties of functional expressed lipase B. Biotechnol Tech 11:689-695.
9. Cygler M, Schrag JD. 1999. Structure and conformational flexibility of Candida rugosa lipase. Biochim Biophys Acta 1441:205-214.
10. Cygler M, Schrag JD, Sussman JL, Harel M, Silman I, Gentry MK, Doctor BP. 1993. Relationship between sequence conservation and three-dimensional structure in a large family of esterases, lipases, and related proteins. Protein Sci 2:366-382.
11. Hoegh I, Patkar S, Halkier T, Hansen MT. 1995. Two lipases from Candida antarctica: Cloning and expression in Aspergillus oryzae. Can J Bot 73:S869-S875.
12. Holmquist M, Tessier DC, Cygler M. 1997. High-level production of recombinant Geotrichum candidum lipases in yeast Pichia pastoris. Protein Expr Purif 11:35-40.
13. Huge-Jensen B, Andreasen F, Christensen T, Christensen M, Thim L, Boel E. 1989. Rhizomucor miehei triglyceride lipase is processed and secreted from transformed Aspergillus oryzae. Lipids 24:781-785.
14. Kaiser R, Erman M, Duax WL, Ghosh D, Jörnvall H. 1994. Monomeric and dimeric forms of cholesterol esterase from Candida cylindracea. FEBS Lett 337:123-127.
15. Keränen S, Penttilä M. 1995. Production of recombinant proteins in the filamentous fungus Trichoderma reesei. Curr Opin Biotechnol 6:534-537.
16. Kiiskinen L-L, Kruus K, Bailey M, Ylösmäki E, Siika-aho M, Saloheimo M. 2004. Expression of Melanocarpus albomyces laccase in Trichoderma reesei and characterization of the purified enzyme. Microbiology 150:3065-3074.
17. Kiiskinen L-L, Saloheimo M. 2004. Molecular cloning and expression in Saccharomyces cerevisiae of a laccase gene from the ascomycete Melanocarpus albomyces. Appl Environ Microbiol 70:137-144.
18. Kiiskinen L-L, Viikari L, Kruus K. 2002. Purification and characterisation of a novel laccase from the ascomycete Melanocarpus albomyces. Appl Microbiol Biotechnol 59:198-204.
19. Kontkanen H, Tenkanen M, Reinikainen T. 2005. Purification and characterisation of a novel steryl esterase from Melanocarpus albomyces. Enzyme Microb Technol, in press.
20. Liew MYB, Ghazali HM, Long K, Lai OM, Yazid AM. 2001. Physical properties of palm kernel olein-anhydrous milk fat mixtures transesterified using mycelium-bound lipase from Rhizomucor miehei. Food Chem 72:447-454.
21. Long K, Ghazali HM, Ariff A, Man YC, Bucke C. 1998. Substrate preference of mycelium-bound lipase from a strain of Aspergillus flavus Link. Biotechnol Lett 20:369-372.
22. Lotti M, Grandori R, Fusetti F, Longhi S, Brocca S, Tramontano A, Alberghina L. 1993. Cloning and analysis of Candida cylindracea lipase sequences. Gene 124:45-55.
23. Minning S, Schmidt-Dannert C, Schmid RD. 1998. Functional expression of Rhizopus oryzae lipase in Pichia pastoris: High-level production and some properties. J Biotechnol 66:147-156.
24. Minning S, Serrano A, Ferrer P, Solá C, Schmid RD, Valero F. 2001. Optimization of the high-level production of Rhizopus oryzae lipase in Pichia pastoris. J Biotechnol 86:59-70.
25. Molinari F, Gandolfi R, Converti A, Zilli M. 2000. Mycelium-bound carboxylesterase from Aspergillus oryzae: An efficient catalyst for acetylation in organic solvent. Enzyme Microb Technol 27:626-630.
26. Montenecourt BS, Eveleigh DE. 1979. Selective screening methods for the isolation of high yielding mutants of Trichoderma reesei. Adv Chem Ser 181:289-301.
27. Nishimura M, Sugiyama M. 1994. Cloning and sequence analysis of a Streptomyces cholesterol esterase gene. Appl Microbiol Biotechnol 41:419-424.
28. Okawa Y, Yamaguchi T. 1977. Studies on sterol-ester hydrolase from Fusarium oxysporum. J Biochem 81:1209-1215.
29. Ollis DL, Cheah E, Cygler M, Dijkstra B, Frolow F, Franken SM, Harel M, Remington SJ, Silman I, Schrag JD, Sussman JL, Verschueren KHG, Goldman A. 1992. The α/β hydrolase fold. Protein Eng 5:197-211.
30. Penttilä M. 1998. Heterologous protein production in Trichoderma. In: Harman G, Kubicek C, editors. Trichoderma and Gliocladium, Vol. 2. London: Taylor & Francis Ltd. p 365-382.
31. Penttilä M, Nevalainen H, Rättö M, Salminen E, Knowles J. 1987. A versatile transformation system for cellulolytic filamentous fungus Trichoderma reesei. Gene 61:155-164.
32. Quyen DT, Schmidt-Dannert C, Schmid RD. 2003. High-level expression of a lipase from Bacillus thermocatenulatus BTL2 in Pichia pastoris and some properties of the recombinant lipase. Protein Expr Purif 28:102-110.
33. Rotticci-Mulder JC, Gustavsson M, Holmquist M, Hult K, Martinelle M. 2001. Expression in Pichia pastoris of Candida antarctica lipase B and lipase B fused to a cellulose-binding domain. Protein Expr Purif 21:386-392.
34. Rúa ML, Schmidt-Dannert C, Wahl S, Sprauer A, Schmid RD. 1997. Thermoalkalophilic lipase of Bacillus thermocatenulatus. Large-scale production, purification and properties: Aggregation behaviour and its effect on activity. J Biotechnol 56:89-102.
35. Sambrook J, Russel DW. 2001. Molecular cloning: A laboratory manual, 3rd edn. NY: Cold Spring Harbor Laboratory Press, Cold Spring Harbor.
36. Sarda L, Desnuelle P. 1958. Action de la lipase pancréatique sur les esters en émulsion. Biochim Biophys Acta 30:513-521.
37. Schmid RD, Verger R. 1998. Lipases: Interfacial enzymes with attractive applications. Angew Chem Int Ed 37:1608-1633.
38. Sugihara A, Shimada Y, Nomura A, Terai T, Imayasu M, Nagai Y, Nagao T, Watanabe Y, Tominaga Y. 2002. Purification and characterization of a novel cholesterol esterase from Pseudomonas aeruginosa, with its application to cleaning lipid-stained contact lenses. Biosci Biotechnol Biochem 66:2347-2355.
39. Taketani S, Nishino T, Katsuki H. 1981. Purification and properties of sterolester hydrolase from Saccharomyces cerevisiae. J Biochem 89:1667-1673.
40. Tang S-J, Shaw J-F, Sun K-H, Sun G-H, Chang T-Y, Lin C-K, Lo Y-C, Lee G-C. 2001. Recombinant expression and characterization of the Candida rugosa lip4 lipase in Pichia pastoris: Comparison of glycosylation, activity, and stability. Arch Biochem Biophys 387:93-98.
41. Tenkanen M, Kontkanen H, Isoniemi R, Spetz P, Holmbom B. 2002. Hydrolysis of steryl esters by a lipase (Lip 3) from Candida rugosa. Appl Microbiol Biotechnol 60:120-127.
42. Uwajima T, Terada O. 1976. Purification and properties of cholesterol esterase from Pseudomonas fluorescens. Agr Biol Chem 40:1957-1964.