Purification and Assays of Saccharomyces cerevisiae Homologous Recombination Proteins

Methods in Enzymology

Volumn 408, Issue , 2006, Pages 445-463

  Van Komen, Stephen ^a   Macris, Margaret ^a   Sehorn, Michael G ^a   Sung, Patrick ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

DOUBLE STRANDED DNA; RECOMBINASE;

BIOCHEMISTRY; CHROMOSOME; DNA STRAND BREAKAGE; EUKARYOTE; HOMOLOGOUS RECOMBINATION; HUMAN; NONHUMAN; PRIORITY JOURNAL; PROTEIN INTERACTION; PURIFICATION; REVIEW; SACCHAROMYCES CEREVISIAE; YEAST;

DNA DAMAGE; DNA REPAIR; DNA REPAIR ENZYMES; DNA, SINGLE-STRANDED; RAD51 RECOMBINASE; RAD52 DNA REPAIR AND RECOMBINATION PROTEIN; RECOMBINATION, GENETIC; REPLICATION PROTEIN A; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

EUKARYOTA; SACCHAROMYCES CEREVISIAE;

EID: 33745195456     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0076-6879(06)08028-1     Document Type: Review

References (40)

1. Alexeev A., Mazin A., and Kowalczykowski S.C. Rad54 protein possesses chromatin-remodeling activity stimulated by the Rad51-ssDNA nucleoprotein filament. Nature Struct. Biol. 10 (2003) 182-186
2. Alexiadis V., and Kadonaga J.T. Strand pairing by Rad54 and Rad51 is enhanced by chromatin. Genes Dev. 16 (2002) 2767-2771
3. Brill S.J., and Stillman B. Yeast replication factor-A functions in the unwinding of the SV40 origin of DNA replication. Nature 342 (1989) 92-95
4. Clever B., Interthal H., Schmuckli-Maurer J., King J., Sigrist M., and Heyer W.D. Recombinational repair in yeast: Functional interactions between Rad51 and Rad54 proteins. EMBO J. 16 (1997) 2535-2544
5. Eggler A.L., Inman R.B., and Cox M.M. The Rad51-dependent pairing of long DNA substrates is stabilized by replication protein A. J. Biol. Chem. 277 (2002) 39280-39288
6. Eisen J.A., Sweder K.S., and Hanawalt P.C. Evolution of the SNF2 family of proteins: Subfamilies with distinct sequences and functions. Nucleic Acids Res. 23 (1995) 2715-2723
7. Emery H.S., Schild D., Kellogg D.E., and Mortimer R.K. Sequence of RAD54, a Saccharomyces cerevisiae gene involved in recombination and repair. Gene 104 (1991) 103-106
8. Gupta R.C., Bazemore L.R., Golub E.I., and Radding C.M. Activities of human recombination protein Rad51. Proc. Natl. Acad. Sci. USA 94 (1997) 463-468
9. Henricksen L.A., Umbricht C.B., and Wold M.S. Recombinant replication protein A: Expression, complex formation, and functional characterization. J. Biol. Chem. 269 (1994) 11121-11132
10. Ito H., Fukuda Y., Murata K., and Kimura A. Transformation of intact yeast cells treated with alkali cations. J. Bacteriol. 153 (1983) 163-168
11. Jaskelioff M., Van Komen S., Krebs J.E., Sung P., and Peterson C.L. Rad54p is a chromatin remodeling enzyme required for heteroduplex DNA joint formation with chromatin. J. Biol. Chem. 278 (2003) 9212-9218
12. Jiang H., Xie Y., Houston P., Stemke-Hale K., Mortensen U.H., Rothstein R., and Kodadek T. Direct association between the yeast Rad51 and Rad54 recombination proteins. J. Biol. Chem. 271 (1996) 33181-33186
13. Kingsman S.M., Cousens D., Stanway C.A., Chambers A., Wilson M., and Kingsman A.J. High-efficiency yeast expression vectors based on the promoter of the phosphoglycerate kinase gene. Methods Enzymol. 185 (1990) 329-341
14. Mazin A.V., Bornarth C.J., Solinger J.A., Heyer W.D., and Kowalczykowski S.C. Rad54 protein is targeted to pairing loci by the Rad51 nucleoprotein filament. Mol. Cell 6 (2000) 583-592
15. Mortensen U.H., Bendixen C., Sunjevaric I., and Rothstein R. DNA strand annealing is promoted by the yeast Rad52 protein. Proc. Natl. Acad. Sci. USA 93 (1996) 10729-10734
16. Nakagawa T., Flores-Rozas H., and Kolodner R.D. The MER3 helicase involved in meiotic crossing over is stimulated by single-stranded DNA-binding proteins and unwinds DNA in the 3′ to 5′ direction. J. Biol. Chem. 276 (2001) 31487-31493
17. New J.H., Sugiyama T., Zaitseva E., and Kowalczykowski S.C. Rad52 protein stimulates DNA strand exchange by Rad51 and replication protein A. Nature 391 (1998) 407-410
18. Ogawa T., Yu X., Shinohara A., and Egelman E.H. Similarity of the yeast RAD51 filament to the bacterial RecA filament. Science 259 (1993) 1896-1899
19. Petukhova G., Stratton S., and Sung P. Catalysis of homologous DNA pairing by yeast Rad51 and Rad54 proteins. Nature 393 (1998) 91-94
20. Petukhova G., Sung P., and Klein H. Promotion of Rad51-dependent D-loop formation by yeast recombination factor Rdh54/Tid1. Genes Dev. 14 (2000) 2206-2215
21. Petukhova G., Van Komen S., Vergano S., Klein H., and Sung P. Yeast Rad54 promotes Rad51-dependent homologous DNA pairing via ATP hydrolysis-driven change in DNA double helix conformation. J. Biol. Chem. 274 (1999) 29453-29462
22. Pierce A.J., Stark J.M., Araujo F.D., Moynahan M.E., Berwick M., and Jasin M. Double-strand breaks and tumorigenesis. Trends Cell Biol. 11 (2001) S52-S59
23. Ristic D., Wyman C., Paulusma C., and Kanaar R. The architecture of the human Rad54-DNA complex provides evidence for protein translocation along DNA. Proc. Natl. Acad. Sci. USA 98 (2001) 8454-8460
24. Sherman F. Getting started with yeast. Methods Enzymol. 194 (1991) 3-21
25. Shinohara A., and Ogawa T. Stimulation by Rad52 of yeast Rad51-mediated recombination. Nature 391 (1998) 404-407
26. Sigurdsson S., Van Komen S., Petukhova G., and Sung P. Homologous DNA pairing by human recombination factors Rad51 and Rad54. J. Biol. Chem. 277 (2002) 42790-42794
27. Solinger J.A., and Heyer W.D. Rad54 protein stimulates the postsynaptic phase of Rad51 protein-mediated DNA strand exchange. Proc. Natl. Acad. Sci. USA 98 (2001) 8447-8453
28. Sugiyama T., Zaitseva E.M., and Kowalczykowski S.C. A single-stranded DNA-binding protein is needed for efficient presynaptic complex formation by the Saccharomyces cerevisiae Rad51 protein. J. Biol. Chem. 272 (1997) 7940-7945
29. Sung P. Catalysis of ATP-dependent homologous DNA pairing and strand exchange by yeast RAD51 protein. Science 265 (1994) 1241-1243
30. Sung P. Yeast Rad55 and Rad57 proteins form a heterodimer that functions with replication protein A to promote DNA strand exchange by Rad51 recombinase. Genes Dev. 11 (1997) 1111-1121
31. Sung P. Function of yeast Rad52 protein as a mediator between replication protein A and the Rad51 recombinase. J. Biol. Chem. 272 (1997) 28194-28197
32. Sung P., Krejci L., Van Komen S., and Sehorn M.G. Rad51 recombinase and recombination mediators. J. Biol. Chem. 278 (2003) 42729-42732
33. Sung P., and Robberson D.L. DNA strand exchange mediated by a RAD51ssDNA nucleoprotein filament with polarity opposite to that of RecA. Cell 82 (1995) 453-461
34. Sung P., and Stratton S.A. Yeast Rad51 recombinase mediates polar DNA strand exchange in the absence of ATP hydrolysis. J. Biol. Chem. 271 (1996) 27983-27986
35. Sung P., Trujillo K.M., and Van Komen S. Recombination factors of Saccharomyces cerevisiae. Mutat. Res. 451 (2000) 257-275
36. Swagemakers S.M., Essers J., de Wit J., Hoeijmakers J.H., and Kanaar R. The human RAD54 recombinational DNA repair protein is a double-stranded DNA-dependent ATPase. J. Biol. Chem. 273 (1998) 28292-28297
37. Symington L.S. Role of RAD52 epistasis group genes in homologous recombination and double-strand break repair. Microbiol. Mol. Biol. Rev. 66 (2002) 630-670
38. Van Komen S., Petukhova G., Sigurdsson S., Stratton S., and Sung P. Superhelicity-driven homologous DNA pairing by yeast recombination factors Rad51 and Rad54. Mol. Cell 6 (2000) 563-572
39. Van Komen S., Petukhova G., Sigurdsson S., and Sung P. Functional cross-talk among Rad51, Rad54, and replication protein A in heteroduplex DNA joint formation. J. Biol. Chem. 277 (2002) 43578-43587
40. Yu X., Jacobs S.A., West S.C., Ogawa T., and Egelman E.H. Domain structure and dynamics in the helical filaments formed by RecA and Rad51 on DNA. Proc. Natl. Acad. Sci. USA 98 (2001) 8419-8424