Rad54 protein possesses chromatin-remodeling activity stimulated by the Rad51-ssDNA nucleoprotein filament

Nature Structural Biology

Volumn 10, Issue 3, 2003, Pages 182-186

  Alexeev, Andrei ^a   Mazin, Alexander ^a,b   Kowalczykowski, Stephen C ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b DREXEL UNIVERSITY COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATASE; DOUBLE STRANDED DNA; FUNGAL PROTEIN; NUCLEOPROTEIN; RAD51 PROTEIN; RAD54 PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; CHROMATIN; DNA REPAIR; DNA STRAND; ENZYME ACTIVITY; GENETIC RECOMBINATION; IN VITRO STUDY; NONHUMAN; NUCLEOSOME; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; SACCHAROMYCES CEREVISIAE; SEQUENCE HOMOLOGY;

BIOLOGICAL TRANSPORT; CHROMATIN; DNA REPAIR; DNA, SINGLE-STRANDED; DNA-BINDING PROTEINS; MICROFILAMENT PROTEINS; MICROFILAMENTS; NUCLEOPROTEINS; NUCLEOSOMES; RAD51 RECOMBINASE; RECOMBINATION, GENETIC; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

SACCHAROMYCES CEREVISIAE;

EID: 0037334946     PISSN: 10728368     EISSN: None     Source Type: Journal    
DOI: 10.1038/nsb901     Document Type: Article

References (18)

1. Paques, F. & Haber, J.E. Multiple pathways of recombination induced by double-strand breaks in Saccharomyces cerevisiae. Microbiol. Mol. Biol. Rev. 63, 349-404 (1999).
2. Fyodorov, D.V. & Kadonaga, J.T. The many faces of chromatin remodeling: SWItching beyond transcription. Cell 106, 523-525 (2001).
3. Langst, G. & Becker, P.B. Nucleosome mobilization and positioning by ISWI-containing chromatin-remodeling factors. J. Cell Sci. 114, 2561-2568 (2001).
4. Aalfs, J.D. & Kingston, R.E. What does 'chromatin remodeling' mean? Trends Biochem. Sci. 25, 548-555 (2000).
5. Hamiche, A., Sandaltzopoulos, R., Gdula, D.A. & Wu, C. ATP-dependent histone octamer sliding mediated by the chromatin remodeling complex NURF. Cell 25, 833-842 (1999).
6. Langst, G., Bonte, E.J., Corona, D.F. & Becker, P.B. Nucleosome movement by CHRAC and ISWI without disruption or trans-displacement of the histone octamer. Cell 97, 843-852 (1999).
7. Whitehouse, I. et al. Nucleosome mobilisation catalysed by the yeast SWI/SNF complex. Nature 400, 784-787 (1999).
8. Sugawara, N. et al. DNA structure-dependent requirements for yeast RAD genes in gene conversion. Nature 373, 84-86 (1995).
9. Linxweiler, W. & Horz, W. Reconstitution of mononucleosomes: characterization of distinct particles that differ in the position of the histone core. Nucleic Acids Res. 12, 9395-9413 (1984).
10. Pennings, S., Meersseman, G. & Bradbury, E.M. Mobility of positioned nucleosomes on 5 S rDNA. J. Mol. Biol. 220, 101-110 (1991).
11. Van Komen, S., Petukhova, G., Sigurdsson, S., Stratton, S. & Sung, P. Superhelicity-driven homologous DNA pairing by yeast recombination factors Rad51 and Rad54. Mol. Cell 6, 563-572 (2000).
12. Mazin, A.V., Bornarth, C.J., Solinger, J.A., Heyer, W.D. & Kowalczykowski, S.C. Rad54 protein is targeted to pairing loci by the Rad51 nucleoprotein filament. Mol. Cell 6, 583-592 (2000).
13. Grigoriev, M. & Hsieh, P. A histone octamer blocks branch migration of a Holliday junction. Mol. Cell. Biol. 17, 7139-7150 (1997).
14. Kotani, H. & Kmiec, E.B. Transcription activates RecA-promoted homologous pairing of nucleosomal DNA. Mol. Cell Biol. 14, 1949-1955 (1994).
15. Alexiadis, V. & Kadonaga, J.T. Strand pairing by Rad54 and Rad51 is enhanced by chromatin. Genes Dev. 16, 2767-2771 (2002).
16. Solinger, J.A., Kiianitsa, K. & Heyer, W.D. Rad54, a Swi2/Snf2-like recombinational repair protein, disassembles Rad51:dsDNA filaments. Mol. Cell 10, 1175-1188 (2002).
17. Owen-Hughes, T. et al. Analysis of nucleosome disruption by ATP-driven chromatin remodeling complexes. Methods Mol. Biol. 119, 319-331 (1999).
18. Lorch, Y., LaPointe, J.W. & Kornberg, R.D. Nucleosomes inhibit the initiation of transcription but allow chain elongation with the displacement of histones. Cell 49, 203-210 (1987).