Induction of apoptosis by intracellular potassium ion depletion: Using the fluorescent dye PBFI in a 96-well plate method in cultured lung cancer cells

Toxicology in Vitro

Volumn 20, Issue 6, 2006, Pages 986-994

  Andersson, B ^a   Janson, V ^a   Behnam Motlagh, P ^a   Henriksson, R ^a   Grankvist, K ^a  

^a UMEÅ UNIVERSITY   (Sweden)

Author keywords

Apoptosis; K+ depletion; Mesothelioma; PBFI AM; Small cell lung cancer

Indexed keywords

AMPHOTERICIN B; BUMETANIDE; CASPASE 3; FLUORESCENT DYE; NIGERICIN; OUABAIN; POTASSIUM BINDING BENZOFURAN ISOPHTHALATE; POTASSIUM ION; UNCLASSIFIED DRUG;

APOPTOSIS; ARTICLE; CELL PROLIFERATION; CONCENTRATION RESPONSE; CONTROLLED STUDY; ENZYME ACTIVITY; FLUORESCENCE; HUMAN; HUMAN CELL; LUNG SMALL CELL CANCER; MESOTHELIOMA; NICK END LABELING; POTASSIUM CELL LEVEL; POTASSIUM TRANSPORT;

APOPTOSIS; BENZOFURANS; CASPASE 3; CASPASES; CELL LINE, TUMOR; ETHERS, CYCLIC; HUMANS; IN SITU NICK-END LABELING; LUNG NEOPLASMS; POTASSIUM;

STROPHANTHUS GRATUS;

EID: 33745188225     PISSN: 08872333     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.tiv.2005.12.013     Document Type: Article

References (17)

1. Andersson B., Behnam-Motlagh P., Henriksson R., and Grankvist K. Pharmacological modulation of lung cancer cells for potassium ion depletion. Anticancer Research 25 (2005) 2609-2616
2. Barbiero G., Duranti F., Bonelli G., Amenta J.S., and Baccino F.M. Intracellular ionic variations in the apoptotic death of L cells by inhibitors of cell cycle progression. Experimental Cell Research 217 (1995) 410-418
3. Bergh J., Nilsson K., Ekman R., and Giovanella B. Establishment and characterization of cell lines from human small cell and large cell carcinomas of the lung. Acta et Pathologica Microbiologica et Immunologica Scandinavica Section A 93 (1985) 133-147
4. + efflux in the activation of apoptosis. Journal of Biological Chemistry 272 (1997) 32436-32442
5. + inhibit cytochrome c-dependent formation of the apoptosome. Journal of Biological Chemistry 276 (2001) 41985-41990
6. Chen N., and Reith M.E. Interaction between dopamine and its transporter: role of intracellular sodium ions and membrane potential. Journal of Neurochemistry 3 (2004) 750-765
7. Cook A.L., Frydenberg M., and Haynes J.M. Protein kinase G activation of K(ATP) channels in human-cultured prostatic stromal cells. Cellular Signaling 14 (2002) 1023-1029
8. Hughes Jr. F.M., and Cidlowski J.A. Potassium is a critical regulator of apoptotic enzymes in vitro and in vivo. Advances in Enzyme Regulation 39 (1999) 157-171
9. + suppresses the activation of apoptosis in lymphocytes. Journal of Biological Chemistry 272 (1997) 30567-30576
10. Kasner S.E., and Ganz M.B. Regulation of intracellular potassium in mesangial cells: a fluorescence analysis using the dye, PBFI. American Journal of Physiology 262 (1992) F462-F467
11. + channels induces apoptosis in vascular smooth muscle cells. American Journal of Physiology. Cell Physiology 280 (2001) C970-C979
12. Li Y., Ganta S., von Stein F.B., Mason D.E., Mitchell B.M., and Freeman L.C. 4-Aminopyridine decreases progesterone production by porcine granulosa cells. Reproductive Biology and Endocrinology 1 (2003) 31
13. Marklund S.L., Westman G.N., Lundgren E., and Roos G. Copper- and zinc-containing superoxide dismutase, manganese-containing superoxide dismutase, catalase, and glutathione peroxidase in normal and neoplastic human cell lines and normal human tissues. Cancer Research 42 (1982) 1955-1961
14. Scoltock A.B., and Cidlowski J.A. Activation of intrinsic and extrinsic pathways in apoptotic signaling during UV-C-induced death of Jurkat cells: the role of caspase inhibition. Experimental Cell Research 297 (2004) 212-223
15. Wang X., Xiao A.Y., Ichinose T., and Yu S.P. Effects of tetraethylammonium analogs on apoptosis and membrane currents in cultured cortical neurons. Journal of Pharmacology and Experimental Therapeutics 295 (2000) 524-530
16. Yu S.P., Yeh C.H., Sensi S.L., Gwag J.B., Canzoniero L.M.T., Farhangrazi Z.S., Ying H.S., Tian M., Dugan L.L., and Choi D.W. Mediation of neuronal apoptosis by enhancement of outward potassium current. Science 278 (1997) 114-117
17. + efflux and neuronal apoptosis. Science 284 (1999) 336-339