Construction of a large signature-tagged mini-Tn5 transposon library and its application to mutagenesis of Sinorhizobium meliloti

Applied and Environmental Microbiology

Volumn 72, Issue 6, 2006, Pages 4329-4337

  Pobigaylo, Nataliya ^a   Wetter, Danijel ^a   Szymczak, Silke ^a   Schiller, Ulf ^a,b   Kurtz, Stefan ^a,c   Meyer, Folker ^a   Nattkemper, Tim W ^a   Becker, Anke ^a  

^a BIELEFELD UNIVERSITY   (Germany)

^b MAX PLANCK INSTITUTE FOR POLYMER RESEARCH   (Germany)

^c UNIVERSITY OF HAMBURG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA; MICROBIOLOGY; MUTAGENESIS; NITROGEN;

ALPHA-PROTEOBACTERIUM; GROWTH PATTERNS; MUTANT PHENOTYPES; MUTANTS; SIGNATURE TAGS; SINORHIZOBIUM MELILOTI; TRANSPOSONS;

GENES;

GENETIC ANALYSIS; GENOMICS; NITROGEN FIXATION; RHIZOBACTERIUM;

ALPHAPROTEOBACTERIA; ARTICLE; BACTERIAL GENOME; BACTERIAL GROWTH; BACTERIAL MUTATION; BACTERIAL SURVIVAL; BACTERIUM MUTANT; COMPETITION; DNA BASE COMPOSITION; DNA LIBRARY; FUNCTIONAL GENOMICS; GENE AMPLIFICATION; GENE DISRUPTION; GENE IDENTIFICATION; GENE SEQUENCE; MELTING POINT; MUTAGENESIS; NITROGEN FIXATION; NONHUMAN; NUCLEOTIDE SEQUENCE; SEQUENCE TAG; SEQUENCE TAGGED SITE; SIGNATURE TAGGED MINI TN5 TRANSPOSON; SIGNATURE TAGGED TRANSPOSON MUTAGENESIS; SINORHIZOBIUM MELILOTI; SYMBIOSIS; TRANSPOSON; VALIDATION PROCESS;

BASE SEQUENCE; CLONING, MOLECULAR; DNA TRANSPOSABLE ELEMENTS; GENOME, BACTERIAL; MOLECULAR SEQUENCE DATA; MUTAGENESIS, INSERTIONAL; PLASMIDS; RESTRICTION MAPPING; SINORHIZOBIUM MELILOTI;

PROTEOBACTERIA; SINORHIZOBIUM MELILOTI;

EID: 33745122953     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.03072-05     Document Type: Article

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