Metabolic profiling by 13C-NMR spectroscopy: [1,2-13C2]glucose reveals a heterogeneous metabolism in human leukemia T cells

Biochimie

Volumn 88, Issue 5, 2006, Pages 437-448

  Miccheli, A ^a   Tomassini, A ^a   Puccetti, C ^a   Valerio, M ^a   Peluso, G ^b   Tuccillo, F ^c   Calvani, M ^d   Manetti, C ^a   Conti, F ^a  

^a SAPIENZA UNIVERSITY OF ROME   (Italy)

^b Institute of Protein Biochemistry   (Italy)

^c NATIONAL CANCER INSTITUTE   (Italy)

^d Scientific Department   (Italy)

Author keywords

13C; Cancer; Metabolic profiling; Metabolomics; NMR spectroscopy

Indexed keywords

ALANINE; AMINO ACID; CITRIC ACID; GLUCOSE; GLUCOSE 6 PHOSPHATE DEHYDROGENASE; GLUTAMIC ACID; GLYCINE; ISOTOPE; LACTIC ACID; MALIC ACID; NUCLEOTIDE; PENTOSE; PENTOSE PHOSPHATE; PHOSPHORIBOSYL PYROPHOSPHATE; PROLINE; PYRUVATE CARBOXYLASE; PYRUVATE DEHYDROGENASE; RIBOSE 5 PHOSPHATE; SERINE; TRACER; TRANSKETOLASE; CARBON; GLUTAMINE;

AMINO ACID METABOLISM; ARTICLE; CARBON NUCLEAR MAGNETIC RESONANCE; CELL COMPARTMENTALIZATION; CELL CYCLE PHASE; CELL HETEROGENEITY; CELL POPULATION; CHEMICAL ANALYSIS; CITRIC ACID CYCLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME SYNTHESIS; EVALUATION; GLYCOLYSIS; HUMAN; HUMAN CELL; INFORMATION PROCESSING; LEUKEMIA; LEUKEMIA CELL LINE; LYMPHOCYTE PROLIFERATION; MEASUREMENT; METABOLISM; METABOLITE; METABOLOMICS; MOLECULAR STABILITY; PHENOTYPE; SIGNAL TRANSDUCTION; T LYMPHOCYTE; BIOLOGICAL MODEL; CELL CYCLE; CELL PROLIFERATION; METHODOLOGY; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PATHOLOGY; PATHOPHYSIOLOGY; PENTOSE PHOSPHATE CYCLE; PHYSIOLOGY; T CELL LEUKEMIA;

CARBON ISOTOPES; CELL CYCLE; CELL PROLIFERATION; CITRIC ACID CYCLE; GLUCOSE; GLUTAMINE; GLYCINE; HUMANS; JURKAT CELLS; LACTIC ACID; LEUKEMIA, T-CELL; MAGNETIC RESONANCE SPECTROSCOPY; MODELS, BIOLOGICAL; NUCLEOTIDES; PENTOSE PHOSPHATE PATHWAY; PENTOSES; SERINE;

EID: 33745083035     PISSN: 03009084     EISSN: 61831638     Source Type: Journal    
DOI: 10.1016/j.biochi.2005.10.004     Document Type: Article

References (38)

1. Gatenby R.A., and Gillies R.J. Why do cancers have high aerobic glycolysis?. Nat. Rev. Cancer 4 (2004) 891-899
2. Yamajy Y., Shiotani T., Nakamura H., Hata Y., Hashimoto Y., Nagai M., Fujita J., and Takahara J. Reciprocal alterations of enzymic phenotype of purine and pyrimidine metabolism in induced differentiation of leukemic cells. Adv. Exp. Med. Biol. 370 (1994) 747-751
3. Mazurek S., Boschek C.B., and Eigenbrodt E. The role of phosphometabolites in cell proliferation, energy metabolism, and tumor therapy. J. Bioener. Biomemb. 29 (1997) 315-330
4. Mazurek S., and Eigenbrodt E. The tumor metabolome. Anticancer Res. 23 (2003) 1149-1154
5. Mazurek S., Grimm H., Boschek C.B., Vaupel P., and Eigenbrodt E. Pyruvate kinase type M2: a crossroad in the tumor metabolome. Br. J. Nutr. 87 Suppl.1 (2002) S23-S29
6. Zwersche W., Mazurek S., Massimi P., Banks L., Eigenbrodt E., and Jansen-Durr P. Modulation of type M2 pyruvate kinase activity by the human papillomavirus type 16 E7 oncoprotein. Proc. Natl. Acad. Sci. USA 96 (1999) 1291-1296
7. Cascante M., Centelles J.J., Veech R.L., Lee W.N., and Boros L.G. Role of thiamine (vitamin B-1) and transketolase in tumor cell proliferation. Nutr. Cancer 36 (2000) 150-154
8. Mazurek S., Eigenbrodt E., Failing K., and Steinberg P. Alterations in the glycolytic and glutaminolytic pathways after malignant transformation of rat liver oval cells. J. Cell. Physiol. 181 (1999) 136-146
9. Mazurek S., Zwerschke W., Jansen-Durr P., and Eigenbrodt E. Effects of the human papilloma virus HPV-16 E7 oncoprotein on glycolysis and glutaminolysis: role of pyruvate kinase type M2 and the glycolytic-enzyme complex. Biochem. J. 356 (2001) 247-256
10. Mazurek S., Zwerschke W., Jansen-Durr P., and Eigenbrodt E. Metabolic cooperation between different oncogenes during cell transformation: interaction between activated ras and HPV-16 E7. Oncogene 20 (2001) 6891-6898
11. Le Mellay V., Houben R., Troppmair J., Hagemann C., Mazurek S., Frey U., Beigel J., Weber C., Benz R., Eigenbrodt E., and Rapp U.R. Regulation of glycolysis by Raf protein serine/threonine kinases. Advan. Enzyme Regul. 42 (2002) 317-332
12. Boros L.G., Cascante M., and Lee W.-N.P. Metabolic profiling of cell growth and death in cancer: applications in drug discovery. DDT 7 (2002) 364-372
13. Lee W.-N.P., Boros L.G., Puigjaner J., Bassilian S., Lim S., and Cascante M. Mass isotopomer study of the nonoxidative pathways of the pentose cycle with [1,2-13C2] glucose. Am. J. Physiol. 274 (1998) E843-E851 (Endocrinol. Metab. 37)
14. Griffiths J.R., McSheehy P.M.J., Robinson S.P., Troy H., Chung Y.-L., Leek R.D., Williams K.J., Stratford I.J., Harris A.L., and Stubbs M. Metabolic changes detected by in vivo magnetic resonance studies of HEPA-1 wild-type tumors and tumors deficient in hypoxia-inducible factor-1β (HIF-1β): evidence of an anabolic role for the HIF-1 pathway. Cancer Res. 62 (2002) 688-695
15. Griffin J.L., and Shockcor J.P. Metabolic profiles of cancer cells. Nat. Rev. Cancer 4 (2004) 551-561
16. 31P NMR spectroscopy. Cell. Mol. Biol. 34 (1988) 591-603
17. 31P-NMR study. BBA 1093 (1991) 29-35
18. 13C NMR. J. Biol. Chem. 265 (1990) 12916-12926
19. 13C NMR spectroscopy. Brain Res. 966 (2003) 116-125
20. 13C] glucose metabolism. J. Neurochem. 61 (1993) 315-323
21. 13C-NMR spectroscopy. Brain Res. 765 (1997) 218-227
22. Kansara M., and Berridge M.V. Hemopoietic cell transformation is associated with failure to downregulate glucose uptake during the G2/M phase of the cell cycle. Exp. Cell Res. 293 (2004) 321-330
23. Warburg O. On respiratory impairment in cancer cells. Science 124 (1956) 269-270
24. 1 phase cycle arrest in Eirlich's tumor cells through inhibition of the pentose cycle. FEBS Lett. 456 (1999) 113-118
25. Boros L.G., Puigjaner J., Lee W.-N.P., Brandes J.L., Bassilian S., Yusuf F.I., Williams R.D., Muscarella P., Melvin W.S., and Schirmer W.J. Oxythiamine and dehydroepiandrosterone inhibit the non-oxidative synthesis of ribose and tumor cell proliferation. Cancer Res. 57 (1997) 4242-4248
26. Comin-Anduix B., Boros L.G., Marin S., Boren J., Callol-Massot C., Centelles J.J., Torres J.L., Agel N., Bassilian S., and Cascante M. Fermented wheat germ extract inhibits glycolysis/pentose cycle enzymes and induces apoptosis through poly(ADP-robose) polymerase activation in Jurkat T-cell leukemia tumor cells. J. Biol. Chem. 277 (2002) 46408-46414
27. Moreadith R.W., and Lehinger A.L. The pathways of glutamate and glutamine oxidation by tumor cell mitochondria. J. Biol. Chem. 259 (1984) 6215-6221
28. Piva T.J., and McEvoy-Bowe E. Oxidation of glutamine in HeLa cells: role and control of truncated TCA cycles in tumour mitochondria. J. Cell. Biochem. 68 (1998) 213-225
29. + malic enzyme of tumor mitochondria. J. Biol. Chem. 259 (1984) 6222-6227
30. Maxwell S.A., and Rivera A. Proline oxidase induces apoptosis in tumor cells, and its expression is frequently absent or reduced in renal carcinomas. J. Biol. Chem. 278 (2003) 9784-9789
31. Maxwell S.A., and Davis G.E. Differential gene expression in p53-mediated apoptosis-resistant vs. apoptosis-sensitive tumor cell lines. Proc. Natl. Acad. Sci. USA 97 (2000) 13009-13014
32. Phang J.M. The regulatory functions of proline and pyrroline-5-carboxylic acid. Curr. Top. Cell. Regul. 25 (1985) 91-132
33. Ahmed W., Rahmani M., Dent P., and Grant S. The cyclin-dependent kinase inhibitor p21 (CIP1/WAF1) blocks paclitaxel-induced G2M arrest and attenuates mitochondrial injury and apoptosis in p53-null human leukemia cells. Cell Cycle 3 (2004) 1305-1311
34. Engel M., Mazurek S., Eigenbrodt E., and Welter C. Phosphoglycerate mutase-derived polypeptide inhibits glycolytic flux and induces cell growth arrest in tumor cell lines. J. Biol. Chem. 279 (2004) 35803-35812
35. Rezaul K., Wu L., Mayya V., Wang S.I., and Han D. A systematic characterization of mitochondrial proteome from human T leukemia cells. Mol. Cell. Proteomics 4 (2005) 169-181
36. Shalom-Barak T., and Knaus U.G. A p21-activated kinase-controlled metabolic switch up-regulates phagocyte NADPH oxidase. J. Biol. Chem. 277 (2002) 40659-40665
37. Gururaj A., Barnes C.J., Vadlamudi R.K., and Kumar R. Regulation of phosphoglucomutase 1 phosphorylation and activity by a signaling kinase. Oncogene 23 (2004) 8118-8127
38. Thiel D.A., Reeder M.K., Pfaff A., Coleman T.R., Sells M.A., and Chernoff J. Cell cycle-regulated phosphorylation of p21-activated kinase 1. Curr. Biol. 12 (2002) 1227-1232