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Volumn 17, Issue 9, 2006, Pages 869-874

Simultaneous detection of tau phospho-epitopes with haptenylated antibodies

Author keywords

Alzheimer's disease; Anti digoxin; Anti fluorescein; Carbocyanine; Digoxigenin anti digoxigenin; Hapten anti hapten technique; Neocortex; Tau pathology; Triple immunofluorescence labelling

Indexed keywords

CARBOCYANINE; DIGOXIGENIN; EPITOPE; FLUORESCEIN; HAPTEN ANTIBODY; MONOCLONAL ANTIBODY; PHOSPHOSERINE; PHOSPHOTHREONINE; TAU PROTEIN;

EID: 33745019110     PISSN: 09594965     EISSN: None     Source Type: Journal    
DOI: 10.1097/01.wnr.0000215778.76602.da     Document Type: Article
Times cited : (5)

References (25)
  • 2
    • 0030937952 scopus 로고    scopus 로고
    • The phosphorylation of tau: A critical stage in neurodevelopment and neurodegenerative processes
    • Lovestone S, Reynolds CH. The phosphorylation of tau: a critical stage in neurodevelopment and neurodegenerative processes. Neuroscience 1997; 78:309-324.
    • (1997) Neuroscience , vol.78 , pp. 309-324
    • Lovestone, S.1    Reynolds, C.H.2
  • 3
    • 0025863618 scopus 로고
    • Neuropathological staging of Alzheimer-related changes
    • Braak H, Braak E. Neuropathological staging of Alzheimer-related changes. Acta Neuropathol 1991; 82:239-259.
    • (1991) Acta Neuropathol , vol.82 , pp. 239-259
    • Braak, H.1    Braak, E.2
  • 4
    • 0028946744 scopus 로고
    • Monoclonal antibody AT8 recognises tau protein phosphorylated at both serine 202 and threonine 205
    • Goedert M, Jakes R, Vanmechelen E. Monoclonal antibody AT8 recognises tau protein phosphorylated at both serine 202 and threonine 205. Neurosci Lett 1995; 189:167-170.
    • (1995) Neurosci Lett , vol.189 , pp. 167-170
    • Goedert, M.1    Jakes, R.2    Vanmechelen, E.3
  • 5
    • 0026551711 scopus 로고
    • The switch of tau protein to an Alzheimer-like state includes the phosphorylation of two serine-proline motifs upstream of the microtubule binding region
    • Biernat J, Mandelkow E-M, Schröter C, Lichtenberg-Kraag B, Steiner B, Berling B, et al. The switch of tau protein to an Alzheimer-like state includes the phosphorylation of two serine-proline motifs upstream of the microtubule binding region. EMBO J 1992; 11:1593-1597.
    • (1992) EMBO J , vol.11 , pp. 1593-1597
    • Biernat, J.1    Mandelkow, E.-M.2    Schröter, C.3    Lichtenberg-Kraag, B.4    Steiner, B.5    Berling, B.6
  • 6
    • 0028362458 scopus 로고
    • A sequence of cytoskeleton changes related to the formation of neurofibrillary tangles and neuropil threads
    • Braak E, Braak H, Mandelkow EM. A sequence of cytoskeleton changes related to the formation of neurofibrillary tangles and neuropil threads. Acta Neuropathol 1994; 87:554-567.
    • (1994) Acta Neuropathol , vol.87 , pp. 554-567
    • Braak, E.1    Braak, H.2    Mandelkow, E.M.3
  • 7
    • 0027984739 scopus 로고
    • Epitope mapping of monoclonal antibodies to the paired helical filaments of Alzheimer's disease: Identification of phosphorylation sites in tau protein
    • Goedert M, Jakes R, Crowther RA, Cohen P, Vanmechelen E, Vandermeeren M, et al. Epitope mapping of monoclonal antibodies to the paired helical filaments of Alzheimer's disease: identification of phosphorylation sites in tau protein. Biochem J 1994; 301:871-877.
    • (1994) Biochem J , vol.301 , pp. 871-877
    • Goedert, M.1    Jakes, R.2    Crowther, R.A.3    Cohen, P.4    Vanmechelen, E.5    Vandermeeren, M.6
  • 8
    • 0030750558 scopus 로고    scopus 로고
    • Unique Alzheimer's disease paired helical filament specific epitopes involve double phosphorylation at specific sites
    • Hoffmann R, Lee VM-Y, Leight S, Varga I, Otvos L Jr. Unique Alzheimer's disease paired helical filament specific epitopes involve double phosphorylation at specific sites. Biochemistry 1997; 36:8114-8124.
    • (1997) Biochemistry , vol.36 , pp. 8114-8124
    • Hoffmann, R.1    Lee, V.M.-Y.2    Leight, S.3    Varga, I.4    Otvos Jr., L.5
  • 9
    • 0032521599 scopus 로고    scopus 로고
    • Sequential phosphorylation of tau by glycogen synthase kinase-3β and protein kinase a at Thr212 and Ser214 generates the Alzheimer-specific epitope of antibody AT100 and requires a paired-helical-filament-like conformation
    • Zheng-Fischhöfer Q, Biernat J, Mandelkow E-M, Illenberger S, Godemann R, Mandelkow E. Sequential phosphorylation of tau by glycogen synthase kinase-3β and protein kinase A at Thr212 and Ser214 generates the Alzheimer-specific epitope of antibody AT100 and requires a paired-helical- filament-like conformation. Eur J Biochem 1998; 252:542-552.
    • (1998) Eur J Biochem , vol.252 , pp. 542-552
    • Zheng-Fischhöfer, Q.1    Biernat, J.2    Mandelkow, E.-M.3    Illenberger, S.4    Godemann, R.5    Mandelkow, E.6
  • 10
    • 0036937699 scopus 로고    scopus 로고
    • Specific tau phosphorylation sites correlate with severity of neuronal cytopathology in Alzheimer's disease
    • Augustinack JC, Schneider A, Mandelkow E-M, Hyman BT. Specific tau phosphorylation sites correlate with severity of neuronal cytopathology in Alzheimer's disease. Acta Neuropathol 2002; 103:26-35.
    • (2002) Acta Neuropathol , vol.103 , pp. 26-35
    • Augustinack, J.C.1    Schneider, A.2    Mandelkow, E.-M.3    Hyman, B.T.4
  • 11
    • 0024543531 scopus 로고
    • Multiple immunofluorescence staining techniques. Use of fluoresceinated, biotinylated and unlabelled monoclonal antibodies
    • Van der Loos CM, Das PK, Van den Oord JJ, Houthoff HJ. Multiple immunofluorescence staining techniques. Use of fluoresceinated, biotinylated and unlabelled monoclonal antibodies. J Immunol Methods 1989; 117:45-52.
    • (1989) J Immunol Methods , vol.117 , pp. 45-52
    • Van Der Loos, C.M.1    Das, P.K.2    Van Den Oord, J.J.3    Houthoff, H.J.4
  • 12
    • 0035066332 scopus 로고    scopus 로고
    • Alzheimer's disease: Genes, proteins and therapy
    • Selkoe DJ. Alzheimer's disease: genes, proteins and therapy. Phys Rev 2001; 81:741-766.
    • (2001) Phys Rev , vol.81 , pp. 741-766
    • Selkoe, D.J.1
  • 13
    • 0029610011 scopus 로고
    • The inflammatory response system of brain: Implications for therapy of Alzheimer and other neurodegenerative disorders
    • McGeer PL, McGeer EG. The inflammatory response system of brain: implications for therapy of Alzheimer and other neurodegenerative disorders. Brain Res Rev 1995; 21:195-218.
    • (1995) Brain Res Rev , vol.21 , pp. 195-218
    • McGeer, P.L.1    McGeer, E.G.2
  • 14
    • 0028324321 scopus 로고
    • Digoxigenin-tagged anti-GFAP and multiple labelling of human glia, vessels and β-amyloid
    • Härtig W, Hausen D, Brauer K, Arendt T, Bigl V, Brückner G. Digoxigenin-tagged anti-GFAP and multiple labelling of human glia, vessels and β-amyloid. NeuroReport 1994; 5:573-576.
    • (1994) NeuroReport , vol.5 , pp. 573-576
    • Härtig, W.1    Hausen, D.2    Brauer, K.3    Arendt, T.4    Bigl, V.5    Brückner, G.6
  • 15
    • 0344906159 scopus 로고    scopus 로고
    • Blot analyses and immunocytochemistry of neural antigens with digoxigenylated primary and secondary antibodies
    • Härtig W, Kirazov L, Brückner G, Holzer M, Gärtner U, Bigl V. Blot analyses and immunocytochemistry of neural antigens with digoxigenylated primary and secondary antibodies. Brain Res Prot 1997; 2:35-43.
    • (1997) Brain Res Prot , vol.2 , pp. 35-43
    • Härtig, W.1    Kirazov, L.2    Brückner, G.3    Holzer, M.4    Gärtner, U.5    Bigl, V.6
  • 16
    • 0020035644 scopus 로고
    • A simple, single-step technique for selecting and cloning hybridomas for the production of monoclonal antibodies
    • Davis JM, Pennington JE, Kubler AM, Conscience JK. A simple, single-step technique for selecting and cloning hybridomas for the production of monoclonal antibodies. J Immunol Methods 1982; 50:161-171.
    • (1982) J Immunol Methods , vol.50 , pp. 161-171
    • Davis, J.M.1    Pennington, J.E.2    Kubler, A.M.3    Conscience, J.K.4
  • 17
    • 0017033341 scopus 로고
    • Conjugation of antibodies with fluorochromes: Modification to the standard methods
    • Goding JW. Conjugation of antibodies with fluorochromes: modification to the standard methods. J Immunol Methods 1976; 13:215-226.
    • (1976) J Immunol Methods , vol.13 , pp. 215-226
    • Goding, J.W.1
  • 18
    • 0028875677 scopus 로고
    • Digoxigenylated primary antibodies for sensitive dual-peroxidase labelling of neuronal markers
    • Härtig W, Brückner G, Holzer M, Brauer K, Bigl V. Digoxigenylated primary antibodies for sensitive dual-peroxidase labelling of neuronal markers. Histochem Cell Biol 1995; 104:467-472.
    • (1995) Histochem Cell Biol , vol.104 , pp. 467-472
    • Härtig, W.1    Brückner, G.2    Holzer, M.3    Brauer, K.4    Bigl, V.5
  • 19
    • 0033022410 scopus 로고    scopus 로고
    • Reduction of lipofuscin-like autofluorescence in fluorescently labeled tissue
    • Schnell SA, Staines WA, Wessendorf MW. Reduction of lipofuscin-like autofluorescence in fluorescently labeled tissue. J Histochem Cytochem 1999; 47:719-730.
    • (1999) J Histochem Cytochem , vol.47 , pp. 719-730
    • Schnell, S.A.1    Staines, W.A.2    Wessendorf, M.W.3
  • 20
    • 10944223484 scopus 로고    scopus 로고
    • Tau protein as a differential biomarker of tauopathies
    • Sergeant N, Delacourte A, Buée L. Tau protein as a differential biomarker of tauopathies. Biochim Biophys Acta 2005; 1739:179-197.
    • (2005) Biochim Biophys Acta , vol.1739 , pp. 179-197
    • Sergeant, N.1    Delacourte, A.2    Buée, L.3
  • 22
    • 10944241542 scopus 로고    scopus 로고
    • Tau alteration and neuronal degeneration in tauopathies: Mechanisms and models
    • Brandt R, Hundelt M, Shahani N. Tau alteration and neuronal degeneration in tauopathies: mechanisms and models. Biochim Biophys Acta 2005; 1739:331-354.
    • (2005) Biochim Biophys Acta , vol.1739 , pp. 331-354
    • Brandt, R.1    Hundelt, M.2    Shahani, N.3
  • 23
    • 0342626774 scopus 로고    scopus 로고
    • Abnormally phosphorylated protein tau in the cortex of aged individuals of various mammalian orders
    • Härtig W, Klein C, Brauer K, Schüppel K-F, Arendt T, Brückner G, et al. Abnormally phosphorylated protein tau in the cortex of aged individuals of various mammalian orders. Acta Neuropathol 2000; 100:305-312.
    • (2000) Acta Neuropathol , vol.100 , pp. 305-312
    • Härtig, W.1    Klein, C.2    Brauer, K.3    Schüppel, K.-F.4    Arendt, T.5    Brückner, G.6
  • 24
    • 0042125603 scopus 로고    scopus 로고
    • Reversible paired helical filament-like phosphorylation is an adaptive process associated with neuronal plasticity in hibernating animals
    • Arendt T, Stieler J, Strijkstra AM, Hut RA, Rüdiger J, Van der Zee EA, et al. Reversible paired helical filament-like phosphorylation is an adaptive process associated with neuronal plasticity in hibernating animals. J Neurosci 2003; 18:6972-6981.
    • (2003) J Neurosci , vol.18 , pp. 6972-6981
    • Arendt, T.1    Stieler, J.2    Strijkstra, A.M.3    Hut, R.A.4    Rüdiger, J.5    Van Der Zee, E.A.6
  • 25
    • 17044439021 scopus 로고    scopus 로고
    • Alzheimer's disease: Aβ, tau and synaptic dysfunction
    • LaFerla FM, Oddo S. Alzheimer's disease: Aβ, tau and synaptic dysfunction. Trends Mol Med 2005; 11:170-176.
    • (2005) Trends Mol Med , vol.11 , pp. 170-176
    • LaFerla, F.M.1    Oddo, S.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.