Optimizing conditions and avoiding pitfalls for prolonged axonal tracing with carbocyanine dyes in fixed rat spinal cords

Journal of Neuroscience Methods

Volumn 154, Issue 1-2, 2006, Pages 256-263

  Chen, Bingkun K ^a,b   Miller, Steven M ^a   Mantilla, Carlos B ^a   Gross, LouAnn ^a   Yaszemski, Michael J ^a   Windebank, Anthony J ^a  

^a MAYO GRADUATE SCHOOL   (United States)

^b JIAMUSI UNIVERSITY   (China)

Author keywords

Axon tracing; DiI; DiO; Spinal cord injury; Trans cellular migration

Indexed keywords

1,1' DIOCTADECYL 3,3,3',3' TETRAMETHYLINDOCARBOCYANINE; 3,3' DIOCTADECYLOXACARBOCYANINE PERCHLORATE; CARBOCYANINE; PARAFORMALDEHYDE; SILASTIC; UNCLASSIFIED DRUG; WAX;

ANIMAL TISSUE; AQUEOUS SOLUTION; ARTICLE; CADAVER; CELL MIGRATION; CONFOCAL MICROSCOPY; CONTROLLED STUDY; CRYSTAL; DIFFUSION; EMBEDDING; HIGH TEMPERATURE; INCUBATION TIME; INTRAMEDULLARY NAILING; LIQUID CULTURE; MATERIAL COATING; MYELIN SHEATH; NERVE FIBER MEMBRANE; NONHUMAN; PRIORITY JOURNAL; RAT; ROOM TEMPERATURE; SILICONE PROSTHESIS; SPINAL CORD; TISSUE SECTION;

ANIMALS; AXONS; CARBOCYANINES; COLORING AGENTS; DIFFUSION; FEMALE; FIXATIVES; FORMALDEHYDE; MICROSCOPY, CONFOCAL; NERVE REGENERATION; RATS; RATS, SPRAGUE-DAWLEY; SPINAL CORD; TEMPERATURE; TISSUE FIXATION;

EID: 33744549075     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2005.12.025     Document Type: Article

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