A novel method for simultaneous anterograde and retrograde labeling of spinal cord motor tracts in the same animal

Journal of Histochemistry and Cytochemistry

Volumn 49, Issue 9, 2001, Pages 1111-1122

  Tsai, E C ^a   Van Bendegem, R L ^a   Hwang, S W ^a   Tator, C H ^a  

^a TORONTO WESTERN HOSPITAL   (Canada)

Author keywords

Adult rats; Anterograde tracing; Axon regeneration; Decalcification; DiI; Fluoro Gold; Immunohistochemistry; Retrograde tracing; Spinal cord repair

Indexed keywords

DEXTRAN; DYE; FLUOROGOLD;

ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; FEMALE; HISTOLOGY; IMMUNOHISTOCHEMISTRY; NERVE FIBER REGENERATION; NONHUMAN; PRIORITY JOURNAL; RAT; SPINAL CORD; TECHNIQUE;

ANIMALIA;

EID: 0034869395     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1177/002215540104900905     Document Type: Article

References (53)

1. Role of group I metabotropic glutamate receptors in traumatic spinal cord white matter injury
2. Differential sites of origin and collateralization of corticospinal neurons in the rat: A multiple fluorescent retrograde tracer study
3. Lack of evidence that myelin-associated glycoprotein is a major inhibitor of axonal regeneration in the CNS
4. Photoconversion of fluorescent retrograde tracers
5. Spontaneous longitudinally orientated axonal regeneration is associated with the Schwann cell framework within the lesion site following spinal cord compression injury of the rat
6. Spinal cord repair in adult paraplegic rats: Partial restoration of hind limb function
7. Direct projections from the lumbosacral spinal cord to Barrington's nucleus in the rat: A special reference to micturition reflex
8. Characterization of commissural interneurons in the lumbar region of the neonatal rat spinal cord
9. Double-labeling of tissue containing the carbocyanine dye DiI for immunocytochemistry
10. Influence of the axotomy to cell body distance in rat rubrospinal and spinal motoneurons: Differential regulation of GAP-43, tubulins, and neurofilament-M
11. Transcellular labeling of taste bud cells by carbocyanine dye (DiI) applied to peripheral nerves in the barbels of the catfish, Ictalurus punctatus
12. Cellular and molecular mechanisms of glial scarring and progressive cavitation: In vivo and in vitro analysis of inflammation-induced secondary injury after CNS trauma
13. Dextran amines in neuronal tracing
14. The development of ipsilateral retinal projections into the tectum in the cichlid fish Haplochromis burtoni: A DiI study in fixed tissue
15. A study in developing visual systems with a new method of staining neurones and their processes in fixed tissue
16. The ability of human Schwann cell grafts to promote regeneration in the transected nude rat spinal cord
17. DiI tracing in combination with immunocytochemistry for analysis of connectivities and chemoarchitectonics of specific neural systems in a teleost, the Atlantic salmon
18. DiI and diO: Versatile fluorescent dyes for neuronal labelling and pathway tracing
19. Survival of chronically-injured neurons can be prolonged by treatment with neurotrophic factors
20. A therapeutic vaccine approach to stimulate axon regeneration in the adult mammalian spinal cord
21. Development of specificity in corticospinal connections by axon collaterals branching selectively into appropriate spinal targets
22. Degenerative changes of the primary trigeminal axons and neurons following infraorbital nerve transection
23. The NG2 chondroitin sulfate proteoglycan: A multifunctional proteoglycan associated with immature cells
24. Schwann cells induce sprouting in motor and sensory axons in the adult rat spinal cord
25. Carbocyanine postmortem neuronal tracing. Influence of different parameters on tracing distance and combination with immunocytochemistry
26. Retrograde transport of fluoro-gold in corticospinal and rubrospinal neurons 10 and 20 weeks after T-9 spinal cord transection
27. Tetramethyl benzidine for horseradish peroxidase neurohistochemistry: A non-carcinogenic blue reaction product with superior sensitivity for visualizing neural afferents and efferents
28. Comparison of horseradish peroxidase visualization methods: Quantitative results and further technical specifics
29. Effect of brain-derived neurotrophic factor, nerve growth factor, and neurotrophin-3 on functional recovery and regeneration after spinal cord injury in adult rats
30. Retrograde tracing with Fluoro-Gold: Different methods of tracer detection at the ultrastructural level and neurodegenerative changes of back-filled neurons in long-term studies
31. Persistent neuronal labeling by retrograde fluorescent tracers: A comparison between Fast Blue, Fluoro-Gold and various dextran conjugates
32. Cortical axons branch to multiple subcortical targets by interstitial axon budding: Implications for target recognition and "waiting periods"
33. DiI labeling combined with conventional immunocytochemical techniques for correlated light and electron microscopic studies
34. Use of lipophilic dyes in studies of axonal pathfinding in vivo
35. Cellular inflammatory response after spinal cord injury in Sprague-Dawley and Lewis rats
36. Functional recovery of paraplegic rats and motor axon regeneration in their spinal cords by olfactory ensheathing glia
37. Efficacy of seven retrograde tracers, compared in multiple-labelling studies of feline motoneurones
38. Visualization of axonally transported horseradish peroxidase using enhanced immunocytochemical detection: A direct comparison with the tetramethylbenzidine method
39. Colocalization of VIP with AVP in neurons of the human paraventricular, supraoptic and suprachiasmatic nucleus
40. Fluoro-Gold: A new fluorescent retrograde axonal tracer with numerous unique properties
41. Neurotrophin-3 enhances sprouting of corticospinal tract during development and after adult spinal cord lesion
42. Axonal regeneration in the rat spinal cord produced by an antibody against myelin-associated neurite growth inhibitors
43. Reduction of lipofuscin-like autofluorescence in fluorescently labeled tissue
44. Increased calpain I-mediated proteolysis, and preferential loss of dephosphorylated NF200, following traumatic spinal cord injury
45. Observations on the survival of grafted embryonic motoneurons in the spinal cord of developing rats
46. Regeneration of dorsal root axons into experimentally altered glial environments in the rat spinal cord
47. Alpha calcium/calmodulin-dependent protein kinase II immunoreactivity in corticospinal neurons: Combination of axonal transport method and immunofluorescence
48. Persistence of rubrospinal projections following spinal cord injury in the rat
49. Recent techniques for tracing pathways in the central nervous system of developing and adult mammals
50. Neuronal tracing with DiI: Decalcification, cryosectioning, and photoconversion for light and electron microscopic analysis
51. Ipsilateral retinal projections into the tectum during regeneration of the optic nerve in the cichlid fish Haplochromis burtoni: A DiI study in fixed tissue
52. Combination of anterograde tracing with Phaseolus vulgaris-leucoagglutinin, retrograde fluorescent tracing and fixed-slice intracellular injection of lucifer yellow
53. Fluorescent tracers as potential candidates for double labeling of descending brain neurons in larval lamprey