Ultrasensitive quantitative HIV-1 p24 antigen assay adapted to dried plasma spots to improve treatment monitoring in low-resource settings

Journal of Clinical Virology

Volumn 36, Issue 1, 2006, Pages 64-67

  Knuchel, Marlyse C ^a   Tomasik, Zuzana ^a   Speck, Roberto F ^b   Lüthy, Ruedi ^c   Schüpbach, Jörg ^a  

^a UNIVERSITY OF ZURICH   (Switzerland)

^b UNIVERSITY HOSPITAL ZURICH   (Switzerland)

^c Swiss AIDS Care International ^*  (Zimbabwe)

Author keywords

Dried plasma spots; HIV 1; Low resource setting; p24 antigen; Treatment monitoring; Viral load

Indexed keywords

ANTIGEN P24; VIRUS RNA; ANTI HUMAN IMMUNODEFICIENCY VIRUS AGENT; GAG PROTEIN; HUMAN IMMUNODEFICIENCY VIRUS ANTIBODY; HUMAN IMMUNODEFICIENCY VIRUS ANTIGEN;

ARTICLE; CORRELATION ANALYSIS; DIAGNOSTIC ACCURACY; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN IMMUNODEFICIENCY VIRUS 1; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; VIRUS IDENTIFICATION; VIRUS LOAD; ADULT; BLOOD; CASE CONTROL STUDY; CHEMISTRY; CHILD; COMPARATIVE STUDY; COST; ECONOMICS; EVALUATION; HEAT; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; IMMUNOLOGY; METHODOLOGY; PROTEIN DENATURATION; SENSITIVITY AND SPECIFICITY; SERODIAGNOSIS; TREATMENT OUTCOME; VIROLOGY;

ADULT; AIDS SERODIAGNOSIS; ANTI-HIV AGENTS; CASE-CONTROL STUDIES; CHILD; COSTS AND COST ANALYSIS; ENZYME-LINKED IMMUNOSORBENT ASSAY; EVALUATION STUDIES; HEAT; HIV ANTIBODIES; HIV ANTIGENS; HIV CORE PROTEIN P24; HIV INFECTIONS; HIV SEROPOSITIVITY; HIV-1; HUMANS; PROTEIN DENATURATION; SENSITIVITY AND SPECIFICITY; TREATMENT OUTCOME; VIRAL LOAD;

EID: 33744520712     PISSN: 13866532     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jcv.2005.12.005     Document Type: Article

References (19)

1. Behets F., Kashamuka M., Pappaioanou M., Green T.A., Ryder R.W., Batter V., et al. Stability of human immunodeficiency virus type 1 antibodies in whole blood dried on filter paper and stored under various tropical conditions in Kinshasa. Zaire J Clin Microbiol 30 (1992) 1179-1182
2. Burgisser P., Vernazza P., Flepp M., Boni J., Tomasik Z., Hummel U., et al. Performance of five different assays for the quantification of viral load in persons infected with various subtypes of HIV-1. Swiss HIV Cohort Study. J Acquir Immune Defic Syndr 23 (2000) 138-144
3. de Baets A.J., Edidi B.S., Kasali M.J., Beelaert G., Schrooten W., Litzroth A., et al. Pediatric human immunodeficiency virus screening in an African district hospital. Clin Diagn Lab Immunol 12 (2005) 86-92
4. Friedl A.C., Ledergerber B., Flepp M., Hirschel B., Telenti A., Furrer H., et al. Response to first protease inhibitor- and efavirenz-containing antiretroviral combination therapy. The Swiss HIV Cohort Study. AIDS 15 (2001) 1793-1800
5. Ledergerber B., Flepp M., Boni J., Tomasik Z., Cone R.W., Luthy R., et al. Human immunodeficiency virus type 1 p24 concentration measured by boosted ELISA of heat-denatured plasma correlates with decline in CD4 cells, progression to AIDS, and survival: comparison with viral RNA measurement. J Infect Dis 181 (2000) 1280-1288
6. Li C.C., Seidel K.D., Coombs R.W., and Frenkel L.M. Detection and quantification of human immunodeficiency virus type 1 p24 antigen in dried whole blood and plasma on filter paper stored under various conditions. J Clin Microbiol 43 (2005) 3901-3905
7. Mascolini M. Transfer of HIV diagnostic and monitoring technologies into resource-poor settings, http://www.hivforum.org/publications/TechTransFinal.pdf; 2002.
8. Respess R.A., Cachafeiro A., Withum D., Fiscus S.A., Newman D., Branson B., et al. Evaluation of an ultrasensitive p24 antigen assay as a potential alternative to human immunodeficiency virus type 1 RNA viral load assay in resource-limited settings. J Clin Microbiol 43 (2005) 506-508
9. Respess R.A., Cachafeiro A., Fiscus S.A., Newman D., Branson B., Varnier O., et al. Evaluation of a commercially available ultrasensitive p24 antigen viral load assay in samples from patients infected with genetically diverse HIV-1 from different geographic settings. 10th conference on retroviruses and opportunistic infections. Boston (2003)
10. Ribas S.G., Ondoa P., Schupbach J., van der Groen G., and Fransen K. Performance of a quantitative human immunodeficiency virus type 1 p24 antigen assay on various HIV-1 subtypes for the follow-up of human immunodeficiency type 1 seropositive individuals. J Virol Methods 113 (2003) 29-34
11. Schupbach J. Viral RNA and p24 antigen as markers of HIV disease and antiretroviral treatment success. Int Arch Allergy Immunol 132 (2003) 196-209
12. Schupbach J., Boni J., Bisset L.R., Tomasik Z., Fischer M., Gunthard H.F., et al. HIV-1 p24 antigen is a significant inverse correlate of CD4 T-cell change in patients with suppressed viremia under long-term antiretroviral therapy. J Acquir Immune Defic Syndr 33 (2003) 292-299
13. Schupbach J., Gunthard H.F., Joos B., Fischer M., Tomasik Z., Boni J., et al. HIV-1 p24 may persist during long-term HAART, increases little during short treatment breaks, and its rebound after treatment stop correlates with the CD4+ loss. J Acquir Immune Defic Syndr 40 (2005) 250-256
14. Sherman G.G., Stevens G., Jones S.A., Horsfield P., and Stevens W.S. Dried blood spots improve access to HIV diagnosis and care for infants in low-resource settings. J Acquir Immune Defic Syndr 38 (2005) 615-617
15. Sterling T.R., Hoover D.R., Astemborski J., Vlahov D., Bartlett J.G., and Schupbach J. Heat-denatured human immunodeficiency virus type 1 protein 24 antigen: prognostic value in adults with early-stage disease. J Infect Dis 186 (2002) 1181-1185
16. Stevens G., Rekhviashvili N., Scott L.E., Gonin R., and Stevens W. Evaluation of two commercially available, inexpensive alternative assay used for assessing viral load in a cohort of human immunodeficiency virus type1 subtype C-infected patients form South Africa. J Clin Microbiol 43 (2005) 857-861
17. Stevens R., Pass K., Fuller S., Wiznia A., Noble L., Duva S., et al. Blood spot screening and confirmatory tests for syphilis antibody. J Clin Microbiol 30 (1992) 2353-2358
18. Sutthent R., Gaudart N., Chokpaibulkit K., Tanliang N., Kanoksinsombath C., and Chaisilwatana P. p24 Antigen detection assay modified with a booster step for diagnosis and monitoring of human immunodeficiency virus type 1 infection. J Clin Microbiol 41 (2003) 1016-1022
19. Tehe A, Maurice C, Hanson DL, Borget MY, Abiola N, Maran M, et al. Quantification and evaluation of HIV-1 p24 by a highly improved ELISA as an alternative to HIV-1 RNA measurement in patients receiving antiretroviral therapy in Abidjan, Côte d'Ivoire, submitted for publication.