Yersinia YopJ acetylates and inhibits kinase activation by blocking phosphorylation

Science

Volumn 312, Issue 5777, 2006, Pages 1211-1214

  Mukherjee, Sohini ^a   Keitany, Gladys ^a   Li, Yan ^a,b   Wang, Yong ^a   Ball, Haydn L ^a,b   Goldsmith, Elizabeth J ^b   Orth, Kim ^a  

^a UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

^b UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACETYLATION; AMINO ACIDS; BIODIVERSITY; IMMUNOLOGY; NITROGEN; PROTEINS; REACTION KINETICS; SIGNALING;

BIOLOGICAL SIGNALING; BLOCKING PHOSPHORYLATION; EUKARYOTIC SIGNALING SYSTEMS; MAPK KINASES (MAPKK);

ENZYME INHIBITION;

ACETYL COENZYME A; ACYLTRANSFERASE; AMINO ACID; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; MITOGEN ACTIVATED PROTEIN KINASE KINASE; MITOGEN ACTIVATED PROTEIN KINASE KINASE 6; OUTER MEMBRANE PROTEIN; SERINE; THREONINE; UNCLASSIFIED DRUG; YERSINIA OUTER PROTEIN J;

BACTERIUM; EUKARYOTE; IMMUNE RESPONSE; PROTEIN;

ACETYLATION; AMINO ACID SEQUENCE; ARTICLE; ENZYME INHIBITION; ENZYME PHOSPHORYLATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN FUNCTION; SEQUENCE ANALYSIS; SIGNAL TRANSDUCTION; SINGAPORE; YERSINIA;

ACETYL COENZYME A; ACETYLATION; ACETYLTRANSFERASES; BACTERIAL PROTEINS; CATALYTIC DOMAIN; CELL LINE; CELL-FREE SYSTEM; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME ACTIVATION; EXTRACELLULAR SIGNAL-REGULATED MAP KINASES; HUMANS; I-KAPPA B KINASE; MAP KINASE KINASE 6; MAP KINASE SIGNALING SYSTEM; NF-KAPPA B; PHOSPHORYLATION; RECOMBINANT PROTEINS; SERINE; SIGNAL TRANSDUCTION; THREONINE; YERSINIA;

EUKARYOTA; YERSINIA;

EID: 33744457909     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1126867     Document Type: Article

References (19)

1. B. J. Hinnebusch, Curr. Issues Mol. Biol. 7, 197 (2005).
2. G. I. Viboud, J. B. Bliska, Annu. Rev. Microbiol. 59, 69 (2005).
3. K. Orth, Curr. Opin. Microbiol. 5, 38 (2002).
4. L. Navarro, N. M. Alto, J. E. Dixon, Curr. Opin. Microbiol. 8, 21 (2005).
5. K. Orth et al., Science 285, 1920 (1999).
6. S. Yoon, Z. Liu, Y. Eyobo, K. Orth, J. Biol. Chem. 278, 2131 (2003).
7. K. Orth et al., Science 290, 1594 (2000).
8. L. E. Palmer, A. R. Pancetti, S. Greenberg, J. B. Bliska, Infect. Immun. 67, 708 (1999).
9. L. Deng et al., Cell 103, 351 (2000).
10. Materials and methods can be found on Science Online.
11. G. Hardie, S. Hanks, Eds., The Protein Kinase FactsBook (Academic Press, San Diego, CA, 1995), p. 418.
12. W. Gu, R. G. Roeder, Cell 90, 595 (1997).
13. J. Ding, W. J. McGrath, R. M. Sweet, W. F. Mangel, EMBO J. 15, 1778 (1996).
14. A. M. Bode, Z. Dong, Sci. STKE 2005, re4 (2005).
15. E. Sim, M. Payton, M. Noble, R. Minchin, Hum. Mol. Genet 9, 2435 (2000).
16. E. W. Brooke et al., Bioorg. Med. Chem. 11, 1227 (2003).
17. S. Y. Roth, J. M. Denu, C. D. Allis, Annu. Rev. Biochem. 70, 81 (2001).
18. Single-letter abbreviations for the amino add residues are as follows: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; O, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; and Y, Tyr.
19. We thank M. Phillips, M. Cobb, E. Olson, J. Goldstein, and M. Brown for insightful discussions and critical reading of the manuscript; H. Zui, S. Sprang, L. Hooper, and J. Humphreys for their helpful conversations; Z. J. Chen, M. White, and K. L. Guan for their generous supply of reagents; and the members of the Orth lab, A. Tizenor, A. Haughey, and W. Simpson for their generous support and assistance. S.M. participation was in partial fulfillment of degree requirements. K.O. thanks R. Taussig, M. Phillips, Y. M. Chook, M. Cobb, and R. Bassel-Duby for years of unconditional support and friendship. K.O., S.M., G.K., Y.W., and E.J.G. were supported by grants from NIH National Institute of Allergy and Infectious Diseases (R01-AI056404 and R21-DK072134) and the Welch Research Foundation (I-1561 and I-1128). K.O. is a Beckman Young Investigator and C. C. Caruth Biomedical Scholar.