Crystal structure of the human adenovirus proteinase with its 11 amino acid cofactor

EMBO Journal

Volumn 15, Issue 8, 1996, Pages 1778-1783

  Ding, Jianzhong ^a   McGrath, William J ^b   Sweet, Robert M ^b   Mangel, Walter F ^b  

^a STONY BROOK UNIVERSITY   (United States)

^b BROOKHAVEN NATIONAL LABORATORY   (United States)

Author keywords

Catalytic triad; Convergent evolution; Cysteine proteinase; Peptide cofactor; Viral proteinase

Indexed keywords

CYSTEINE PROTEINASE; PAPAIN; VIRUS ENZYME;

AMINO ACID SEQUENCE; ARTICLE; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME BINDING; ENZYME STRUCTURE; EVOLUTION; HUMAN ADENOVIRUS 2; NONHUMAN; PRIORITY JOURNAL; PROTEIN FOLDING; PROTEIN SECONDARY STRUCTURE; X RAY CRYSTALLOGRAPHY;

EID: 0029914251     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1002/j.1460-2075.1996.tb00526.x     Document Type: Article

References (38)

1. Allaire, M., Chernaia, M.M , Malcolm, B.A and James, M.N G (1994) Picornaviral 3C cysteine proteinases have a fold similar to chymotrypsin-like serine proteinases. Nature, 369, 72-76.
2. Anderson, C.W. (1990) The proteinase polypeptide of adenovirus serotype-2 virions. Virology, 177, 259-272.
3. Anderson, C.W. (1993) Expression and purification of the adenovirus proteinase polypeptide and of a synthetic proteinase substrate. Protein Expression Purif., 4, 8-15
4. Bernstein, F.C., Koetzle, T.F., Williams, G.J.B., Meyer, E.F., Brice, M D., Rodgers, J.R., Kennard, O., Shimanouchi, T. and Tasumi, M. (1977) The protein data bank: A computer-based archival file for macromolecular structures. J. Mol. Biok., 112, 535-542.
5. Blow, D.M., Birktoft, J.J and Hartley, B.S. (1969) Role of a buried acid group in the mechanism of action of chymotrypsin. Nature, 221, 337-340.
6. Brünger, A.T. (1993) X-PLOR, Version 3.1. Yale University.
7. Cai, F and Weber, J M. (1993) Organization of the avian adenovirus genome and the structure of its endopeptidase. Virology, 196, 358-362.
8. Drenth, J., Jansonius, J.N., Koekoek, R and Wolthers, B.G. (1971) The structure of papain. Adv. Protein Chem., 25, 79-115.
9. Drenth, J., Kalk, K.H. and Swen, H.M. (1976) Binding of chloromethyl ketone substrate analogues to crystalline papain. Biochemistry, 15, 3731-3738.
10. Furey, W. and Swaminathan, S. (1990) PHASES: a package of programs for protein crystallography. Am Crystallogr. Assoc. Meeting Abstr., PA33, 18, 73.
11. Hannan, C., Raptis, L.H., Dery, C.V. and Weber, J (1983) Biological and structural studies with adenovirus type 2: temperature-sensitive mutant defective for uncoating Intervirology, 19, 213-223.
12. Holm, L and Sander, C. (1994) The FSSP database of structurally aligned protein fold families. Nucleic Acids Res , 22, 3600-3609.
13. Horwitz, M.S. (1990) In Fields, B.N. and Knipe, D M. (eds), Fields Virology. Raven Press Ltd, New York, pp. 1723-1742.
14. Jones, T.A., Zou, J.Y., Cowan, S.W. and Kjeldgaard, M. (1991) Improved methods for building protein models in electron density maps and the location of errors in these models. Acta Crystallogr., A47, 110-119.
15. Kraulis, P.J. (1991) MOLSCRIPT: a program to produce both detailed and schematic plots of protein structures. J. Appl. Crystallogr., 24, 946-950.
16. Laskowski, R.A., MacArthur, M.W., Moss, D.S. and Thorton, J.M. (1993) PROCHECK: a program to check the stereochemical quality of protein structures. J. Appl. Crystallogr., 26, 283-291.
17. McGrath, W.J., Abola, A P , Toledo, D.L., Brown, M.L., and Mangel, W.F. (1996a) Characterization of human adenovirus proteinase activity in disrupted virus particles. Virology, in press.
18. McGrath, W.J., Ding, J., Sweet, R.M. and Mangel, W.F. (1996b) Crystallization of human adenovirus serotype 2 proteinase with its eleven amino acid cofactor pV1c. J. Struct. Biol., in press.
19. McGrath, W.J , Li, C., McWhirter, S.M., Toledo, D.L. and Mangel, W.F. (1996c) Binding to DNA by the two protein components of human adenovirus. Biochemistry, in press.
20. Mangel, W.E. McGrath, W.J., Toledo, D.L. and Anderson, C.W. (1993) Viral DNA and a viral peptide can act as cofactors of adenovirus virion proteinase activity. Nature, 361, 274-275.
21. Mangel, W.F , Toledo, D.L., Brown, M T., Martin, J.H. and McGrath, W.J. (1996) Characterization of three components of human adenovirus proteinase activity in vitro. J. Biol Chem., 271, 536-543.
22. Mirza, A. and Weber, J. (1980) Infectivity and uncoating of adenovirus cores. Intervirology, 13, 307-311.
23. Nicholl, A. and Honig, B.J (1991) A rapid finite difference algorithm, utilizing successive over-relaxation 10 solve the Poisson-Boltzman equation. J. Comp. Chem., 12, 435-445.
24. Ollis, D.L. et al. (1992) The alpha/beta hydrolase fold. Protein Engng, 5, 197-211.
25. Polgar, L. (1974) Mercaptide-imidazolium ion-pair: the reactive nucleophile in papain catalysis. FEBS Lett., 47, 15-18.
26. Polgar, L. (1989) Mechanisms of Protease Action CRC Press, Inc., Boca Raton, FL.
27. Ramachandran, G.N and Sasisekharan, V. (1968) Conformation of polypeptides and proteins. Adv. Protein Chem., 23, 283-437.
28. Rawlings, N.D. and Barrett, A.J. (1994) Families of cysteine peptidases. Methods Enzymol., 244, 461-486.
29. Robertus, J D., Kraut, J., Alden, R.A. and Birktoft, J.J. (1972) Subtilisin: a stereo-chemical mechanism involving transition-state stabilization. Biochemistry, 11, 4293-4303.
30. Schechter, I. and Berger, A. (1967) On the size of the active site in proteases. I. Papain. Biochem. Biophys. Res. Commun., 27, 157-162.
31. Terwilliger, T.C., Kim, S.-H. and Eisenberg, D. (1987) Generalized method of determining heavy-atom positions using the difference Patterson function. Acta Crystallogr., A43, 1-5.
32. Tihanyi, K., Bourbonniere, M , Houde, A., Rancourt, C. and Weber, J.M. (1993) Isolation and properties of adenovirus type 2 proteinase. J. Biol. Chem., 268, 1780-1785.
33. Wang, B.C. (1985) Resolution of phase ambiguity in macromolecular crystallography Methods Enzymol., 115, 90-112.
34. Weber, J. (1976) Genetic analysis of adenovirus type 2 III. Temperature sensitivity of processing of viral proteins. J. Virol , 17, 462-471
35. Webster, A., Russell, W.C. and Kemp, G.D. (1989) Characterization of the adenovirus proteinase; development and use of a specific peptide assay. J. Gen. Virol., 70, 3215-3223.
36. Webster, A., Russell, S., Talbot, P , Russell, W.C. and Kemp, G.D. (1989) Characterization of the adenovirus proteinase: substrate specificity. J. Gen. Virol., 70, 3225-3234.
37. Webster, A., Hay, R.T. and Kemp, G. (1993) The adenovirus protease is activated by a virus-coded disulphide-linked peptide. Cell, 72, 97-104.
38. Yeh-Kai, L., Akusjarvi, G., Alestrom, P., Petterson, U., Tremblay, M. and Weber, J. (1983) Genetic identification of an endopeptidase encoded by the adenovirus genome. J. Mol. Biol., 167, 217-222.