Cellular stability of Rho-GTPases glucosylated by Clostridium difficile toxin B

FEBS Letters

Volumn 580, Issue 14, 2006, Pages 3565-3569

  Genth, Harald ^a   Huelsenbeck, Johannes ^a   Hartmann, Birgit ^a   Hofmann, Fred ^a   Just, Ingo ^a   Gerhard, Ralf ^a  

^a HANNOVER MEDICAL SCHOOL   (Germany)

Author keywords

Clostridium difficile toxin B; Fibroblasts; Proteasomal degradation; Rac1; RhoA

Indexed keywords

CLOSTRIDIUM DIFFICILE TOXIN B; LACTACYSTIN; PROTEASOME; PROTEIN CDC42; RAC1 PROTEIN; RHO GUANINE NUCLEOTIDE BINDING PROTEIN;

ANIMAL CELL; ARTICLE; CELL LEVEL; CLOSTRIDIUM DIFFICILE; CONTROLLED STUDY; FIBROBLAST; GLYCOSYLATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION;

ANIMALS; BACTERIAL PROTEINS; BACTERIAL TOXINS; CELL-FREE SYSTEM; FIBROBLASTS; GLYCOSYLATION; MICE; NIH 3T3 CELLS; RHO GTP-BINDING PROTEINS;

CLOSTRIDIUM DIFFICILE;

EID: 33646942752     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.febslet.2006.04.100     Document Type: Article

References (18)

1. Wennerberg K., and Der C.J. Rho-family GTPases: it's not only Rac and Rho (and I like it). J. Cell Sci. 117 (2004) 1301-1312
2. Genth H., Gerhard R., Maeda A., Amano M., Kaibuchi K., Aktories K., and Just I. Entrapment of Rho ADP-ribosylated by Clostridium botulinum C3 exoenzyme in the Rho-guanine nucleotide dissociation inhibitor-1 complex. J. Biol. Chem. 278 (2003) 28523-28527
3. Just I., and Gerhard R. Large clostridial cytotoxins. Rev. Physiol. Biochem. Pharmacol. 152 (2004) 23-47
4. Vetter I.R., Hofmann F., Wohlgemuth S., Herrmann C., and Just I. Structural consequences of mono-glucosylation of Ha-Ras by Clostridium sordellii lethal toxin. J. Mol. Biol. 301 (2000) 1091-1095
5. Genth H., Schmidt M., Gerhard R., Aktories K., and Just I. Activation of phospholipase D1 by ADP-ribosylated RhoA. Biochem. Biophys. Res. Commun. 302 (2003) 127-132
6. Hahn A., Barth H., Kress M., Mertens P.R., and Goppelt-Strübe M. Role of Rac and Cdc42 in lysophasphatidic acid-mediated cyclo-oxygenase-2 gene expression. Biochem. J. 362 (2002) 33-40
7. Chaves-Olarte E., Freer E., Parra A., Guzmàn-Verri C., Moreno E., and Thelestam M. R-Ras glucosylation and transient RhoA activation determine the cytopathic effect produced by toxin B variants from toxin A-negative strains of Clostridium difficile. J. Biol. Chem. 278 (2003) 7956-7963
8. Clerk A., Pham F.H., Fuller S.J., Sahai E., Aktories K., Marais R., Marshall C., and Sugden P.H. Regulation of mitogen-activated protein kinases in cardiac myocytes through the small G protein Rac1. Mol. Cell. Biol. 21 (2001) 1173-1184
9. Czeloth N., Bernhardt G., Hofmann F., Genth H., and Forster R. Sphingosine-1-phosphate mediates migration of mature dendritic cells. J. Immunol. 175 (2005) 2960-2967
10. Gerhard R., Tatge H., Genth H., Thum T., Borlak J., Fritz G., and Just I. Clostridium difficile toxin A induces expression of the stress-induced early gene product RhoB. J. Biol. Chem. 280 (2005) 1499-1505
11. Lanning C.C., Daddona J.L., Ruiz-Velasco R., Shafer S.H., and Williams C.L. The Rac1 C-terminal polybasic region regulates the nuclear localization and protein degradation of Rac1. J. Biol. Chem. 279 (2004) 44197-44210
12. Sehr P., Joseph G., Genth H., Just I., Pick E., and Aktories K. Glucosylation and ADP-ribosylation of Rho proteins - effects on nucleotide binding, GTPase activity, and effector-coupling. Biochemistry 37 (1998) 5296-5304
13. Kubori T., and Galan J.E. Temporal regulation of salmonella virulence effector function by proteasome-dependent protein degradation. Cell 115 (2003) 333-342
14. Boquet P., and Lemichez E. Bacterial virulence factors targeting Rho GTPases: parasitism or symbiosis?. Trends Cell Biol. 13 (2003) 238-246
15. Dillon S.T., Rubin E.J., Yakubovich M., Pothoulakis C., LaMont J.T., Feig L.A., and Gilbert R.J. Involvement of Ras-related Rho proteins in the mechanisms of action of Clostridium difficile toxin A and toxin B. Infect. Immun. 63 (1995) 1421-1426
16. Barth H., Olenik C., Sehr P., Schmidt G., Aktories K., and Meyer D.K. Neosynthesis and activation of Rho by Escherichia coli cytotoxic necrotizing factor (CNF1) reverse cytopathic effects of ADP-ribosylated Rho. J. Biol. Chem. 274 (1999) 27407-27414
17. Genth H., Aktories K., and Just I. Monoglucosylation of RhoA at threonine-37 blocks cytosol-membrane cycling. J. Biol. Chem. 274 (1999) 29050-29056
18. Herrmann C., Ahmadian M.R., Hofmann F., and Just I. Functional consequences of monoglucosylation of H-Ras at effector domain amino acid threonine-35. J. Biol. Chem. 273 (1998) 16134-16139