Development of serum-free media for a recombinant CHO cell line producing recombinant antibody

Enzyme and Microbial Technology

Volumn 39, Issue 3, 2006, Pages 426-433

  Kim, Do Yun ^a,b   Lee, Joon Chul ^c   Chang, Ho Nam ^b   Oh, Duk Jae ^a,d  

^a Sejong University   (South Korea)

^b Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

^c Korea Institute of Industrial Technology   (South Korea)

^d Inha University   (South Korea)

Author keywords

Chinese hamster ovary cell; Recombinant antibody production; Serum free medium

Indexed keywords

ANTIBODIES; CONCENTRATION (PROCESS); GENETIC ENGINEERING; INSULIN;

CHINESE HAMSTER OVARY CELL; FORMULATION; RECOMBINANT ANTIBODY PRODUCTION; SERUM-FREE MEDIUM;

PROTEINS;

ASCORBIC ACID; BIOTIN; FERRIC CITRATE; INSULIN; PROTEIN HYDROLYSATE; RECOMBINANT ANTIBODY; TRANSFERRIN;

ANIMAL CELL; ARTICLE; CELL AGGREGATION; CELL DENSITY; CELL GROWTH; CELL VIABILITY; CONTROLLED STUDY; CULTURE MEDIUM; NONHUMAN; VITAMIN SUPPLEMENTATION; YEAST;

ANIMALIA; CRICETULUS GRISEUS;

EID: 33646863660     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2005.11.047     Document Type: Article

References (14)

1. Stoll T.S., Miihlethaler K., Stockar U.V., and Marison I.W. Systematic improvement of a chemically-defined protein-free medium for hybridoma growth and monoclonal antibody production. J Biotechnol 45 (1996) 111-123
2. Lee G.M., Kim E.J., Kim N.S., Yoon S.K., Ahn Y.H., and Song J.Y. Development of a serum-free medium for the production of erythropoietin by suspension culture of recombinant Chinese hamster ovary cells using a statistical design. J Biotechnol 69 (1999) 85-93
3. Schröder M., Matischak K., and Friedl P. Serum- and protein-free media formulations for the Chinese hamster ovary cell line DUKXB11. J Biotechnol 108 (2004) 279-292
4. Chen Z., Iding K., Lütkemeyer D., and Lehmann J. A low-cost chemically defined protein free medium for a recombinant CHO cell line producing prothrombin. Biotechnol Lett 22 (2000) 837-841
5. Sung Y.H., Lim S.W., Chung J.Y., and Lee G.M. Yeast hydrolysate as a low-cost additive to serum-free medium for the production of human thrombopoietin in suspension cultures of Chinese hamster ovary cells. Appl Microbiol Biotechnol 63 (2004) 527-536
6. Liua C.H., Chua I.M., and Hwang S.M. Factorial designs combined with the steepest ascent method to optimize serum-free media for CHO cells. Enzyme Microb Technol 28 (2001) 314-321
7. Ducommun P., Ruffieux P., Stockar U.V., and Marison I. The role of vitamins and amino acids on hybridoma growth and monoclonal antibody production. Cytotechnol 37 (2001) 65-73
8. Xie L., Nyberg G., Gu X., Li H., Möllborn F., and Wang D.C. Gamma-interferon production and quality in stoichiometric fed-batch cultures of Chinese Hamster Ovary (CHO) cells under serum-free conditions. Biotechnol Bioeng 56 (1997) 577-582
9. Xie L., and Wang D.C. High cell density and high monoclonal antibody production through medium design and rational control in a bioreactor. Biotechnol Bioeng 51 (1996) 725-729
10. Chiou T.W., Hsieh Y.C., and Ho C.S. High density culture of insect cells using rational medium design and feeding strategy. Bioprocess Eng 22 (2000) 483-491
11. 3 on serum-free Chinese Hamster Ovary cell cultures expressing two recombinant proteins. Biotechnol Prog 16 (2000) 693-697
12. Kim J.H., Kim E.J., and Park T.H. Fed-batch culture of insect cells with exponential feeding of amino acid and yeastolate solution. Bioprocess Eng 23 (2000) 367-370
13. Ishaque A., and Al-Rubeai M. Role of vitamins in determining apoptosis and extent of suppression by bcl-2 during hybridoma cell culture. Apoptosis 7 (2002) 231-239
14. Kim DY, Lee JC, Chang HN, Oh DJ. Effects of supplementation of various medium components on Chinese Hamster Ovary cell cultures producing recombinant antibody. Cytotechnology 2005;47:37-49.