Identification of a catalytic residue of Clostridium paraputrificum N-acetyl-β-D-glucosaminidase Nag3A by site-directed mutagenesis

Bioscience, Biotechnology and Biochemistry

Volumn 70, Issue 5, 2006, Pages 1127-1133

  Li, Huazhong ^a   Zhao, Guangshan ^b   Miyake, Hideo ^b   Umekawa, Hayato ^b   Kimura, Tetsuya ^b   Ohmiya, Kunio ^b   Sakka, Kazuo ^b  

^a JIANGNAN UNIVERSITY   (China)

^b MIE UNIVERSITY   (Japan)

Author keywords

N acetylglucosaminidase; Catalytic residue; Clostridium paraputrificum; Site directed mutagenesis

Indexed keywords

BINDING ENERGY; CATALYST ACTIVITY; ENZYMES; MUTAGENESIS; PROTONS; SPECTRUM ANALYSIS;

Β-N-ACETYLGLUCOSAMINIDASE; CATALYTIC RESIDUE; CLOSTRIDIUM PARAPUTRIFICUM; SITE-DIRECTED MUTAGENESIS;

BACTERIA;

ACETYLGLUCOSAMINIDASE; ASPARTIC ACID;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; CATALYSIS; CHEMISTRY; CIRCULAR DICHROISM; CLOSTRIDIUM; ENZYME ACTIVE SITE; ENZYME SPECIFICITY; ENZYMOLOGY; GENETICS; ISOLATION AND PURIFICATION; MOLECULAR GENETICS; MUTATION; SITE DIRECTED MUTAGENESIS;

ACETYLGLUCOSAMINIDASE; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ASPARTIC ACID; CATALYSIS; CATALYTIC DOMAIN; CIRCULAR DICHROISM; CLOSTRIDIUM; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTATION; SUBSTRATE SPECIFICITY;

CLOSTRIDIUM; CLOSTRIDIUM PARAPUTRIFICUM;

EID: 33646786475     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.70.1127     Document Type: Article

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