Minimizing loss of sequence information in SAGE ditags by modulating the temperature dependent 3′ → 5′ exonuclease activity of DNA polymerases on 3′-terminal isoheptyl amino groups

Biotechnology and Bioengineering

Volumn 94, Issue 1, 2006, Pages 54-65

  So, Austin P ^a   Turner, Robin F B ^a   Haynes, Charles A ^a,b  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^b UNIVERSITY OF BRITISH COLUMBIA   (Canada)

Author keywords

DNA polymerase; Exonuclease; Phosphorothioate; Recovery of sequence information; Serial analysis of gene expression

Indexed keywords

AMINO ACIDS; AMPLIFICATION; GELS; PHOSPHORUS COMPOUNDS; PURIFICATION; RNA; THERMAL EFFECTS;

DNA POLYMERASE; EXONUCLEASE; PHOSPHOROTHIOATE; RECOVERY OF SEQUENCE INFORMATION; SERIAL ANALYSIS OF GENE EXPRESSION;

DNA;

DNA DIRECTED DNA POLYMERASE BETA; DNA POLYMERASE; EXONUCLEASE; OLIGONUCLEOTIDE; PHOSPHOROTHIOIC ACID;

ARTICLE; ENZYME ACTIVITY; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS; SERIAL ANALYSIS OF GENE EXPRESSION; TEMPERATURE;

3' UNTRANSLATED REGIONS; AMINO ACID SEQUENCE; BACTERIOPHAGE T4; BASE SEQUENCE; COMPUTER SIMULATION; CRYPTOCOCCUS NEOFORMANS; DEOXYRIBONUCLEASES, TYPE II SITE-SPECIFIC; DNA LIGASES; DNA POLYMERASE I; DNA, COMPLEMENTARY; DNA-DIRECTED DNA POLYMERASE; EXONUCLEASES; EXPRESSED SEQUENCE TAGS; GENE LIBRARY; NUCLEIC ACID AMPLIFICATION TECHNIQUES; OLIGONUCLEOTIDES; POLYMERASE CHAIN REACTION; RNA, FUNGAL; RNA, MESSENGER; SEQUENCE ANALYSIS, DNA; TEMPERATURE;

EID: 33646571251     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.20805     Document Type: Article

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