Comparative thermal denaturation of Thermus aquaticus and Escherichia coli type 1 DNA polymerases

Biochemical Journal

Volumn 374, Issue 3, 2003, Pages 785-792

  Karantzeni, Irene ^a,b   Ruiz, Carmen ^a   Liu, Chin Chi ^a   Licata, Vince J ^a  

^a LOUISIANA STATE UNIVERSITY   (United States)

^b ASTRAZENECA   (United Kingdom)

Author keywords

CD; Differential scanning calorimetry; Protein folding; Taq polymerase; Thermophilic

Indexed keywords

DIFFERENTIAL SCANNING CALORIMETRY; DNA; ESCHERICHIA COLI; HIGH TEMPERATURE EFFECTS; METHANOL; PH EFFECTS; POLYPEPTIDES; REACTION KINETICS;

PROTONATION;

ENZYMES;

5' NUCLEASE; DNA POLYMERASE; METHANOL; NUCLEASE; POL 1 POLYMERASE; POLYPEPTIDE; TAQ POLYMERASE; UNCLASSIFIED DRUG; DNA DIRECTED DNA POLYMERASE BETA; ESCHERICHIA COLI PROTEIN;

ARTICLE; CIRCULAR DICHROISM; COMPARATIVE STUDY; CONTROLLED STUDY; DIFFERENTIAL SCANNING CALORIMETRY; ENTHALPY; ESCHERICHIA COLI; HIGH TEMPERATURE; HYDROPHOBICITY; KINETICS; MELTING POINT; MONITORING; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DENATURATION; PROTEIN DOMAIN; PROTEIN FOLDING; PROTEIN INTERACTION; PROTEIN STABILITY; PROTEIN STRUCTURE; PROTON TRANSPORT; STRUCTURE ANALYSIS; THERMAL ANALYSIS; THERMODYNAMICS; THERMOSTABILITY; THERMUS AQUATICUS; CHEMISTRY; ENZYMOLOGY; HEAT; NON-PROGRAMMATIC; THERMUS;

ESCHERICHIA COLI; NEGIBACTERIA; THERMUS; THERMUS AQUATICUS;

NON-PROGRAMMATIC;

CALORIMETRY, DIFFERENTIAL SCANNING; DNA POLYMERASE I; ESCHERICHIA COLI PROTEINS; HEAT; PROTEIN DENATURATION; PROTEIN FOLDING; TAQ POLYMERASE; THERMUS;

EID: 0141567644     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20030323     Document Type: Article

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