Time-resolved optical imaging provides a molecular snapshot of altered metabolic function in living human cancer cell models

Optics Express

Volumn 14, Issue 10, 2006, Pages 4412-4426

  Sud, Dhruv ^a   Zhong, Wei ^a   Beer, David G ^a   Mycek, Mary Ann ^a  

^a UNIVERSITY OF MICHIGAN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELLS; FLUORESCENCE; METABOLISM; OPTICAL PROPERTIES; PHYSIOLOGY; TUMORS;

HUMAN CANCER CELL MODELS; METABOLIC FUNCTION; OPTICAL IMAGING;

IMAGING TECHNIQUES;

EID: 33646552411     PISSN: 10944087     EISSN: 10944087     Source Type: Journal    
DOI: 10.1364/OE.14.004412     Document Type: Article

References (28)

1. "Cancer Facts and Figures 2005," (American Cancer Society, Atlantic City, 2005).
2. I. Georgakoudi, B. C. Jacobson, M. G. Muller, E. E. Sheets, K. Badizadegan, D. L. Carr-Locke, C. P. Crum, C. W. Boone, R. R. Dasari, J. Van Dam, and M. S. Feld, "NAD(P)H and collagen as in vivo quantitative fluorescent biomarkers of epithelial precancerous changes," Cancer Research 62, 682-687 (2002).
3. E. Eigenbrodt, U. Gerbracht, S. Mazurek, P. Presek, and R. Friis, "Carbohydrate metabolism and neoplasia: new perspectives for diagnosis and therapy," in Biochemical and Molecular Aspects of Selected Cancers, T. G. Pretlow II, andT. P. Pretlow, eds. (Academic Press, Inc., San Diego, 1994), 311-385.
4. H. Schneckenburger, and K. König, "Fluorescence decay and imaging of NAD(P)H and flavins as metabolic indicators," Opt Eng 31, 1447-1451 (1992).
5. J. R. Lakowicz, Principles of Fluorescence Spectroscopy (Kluwer Academic/Plenum, New York, 1999).
6. J. W. Dobrucki, "Interaction of oxygen-sensitive luminescent probes Ru(phen)(3)(2+) and Ru(bipy)(3)(2+) with animal and plant cells in vitro. Mechanism of phototoxicity and conditions for non-invasive oxygen measurements," J Photochem Photobiol B 65, 136-144 (2001).
7. J. V. Houten, and R. J. Watts, "Temperature dependence of the photophysical and photochemical properties of the Tris(2,2′-bipyridyl) ruthenium(II) ion in aqueous solution," J Am Chem Soc 96, 4853-4858 (1976).
8. W. Rudolph, and M. Kempe, "Topical review: Trends in optical biomedical imaging," J Mod Opt 44, 1617-1642 (1997).
9. P. J. Tadrous, "Methods for imaging the structure and function of living tissues and cells: 2. fluorescence lifetime imaging," Journal of Pathology 191, 229-234 (2000).
10. Y. Chen, and J. D. Mills, "Protein localization in living cells and tissues using FRET and FLIM," Differentiation 71, 528-541 (2003).
11. R. N. Day, and D. W. Piston, "Spying on the hidden lives of proteins," Nat Biotechnol 17, 425-426 (1999).
12. P. K. Urayama, W. Zhong, J. A. Beamish, F. K. Minn, R. D. Sloboda, K. H. Dragnev, E. Dmitrovsky, and M.-A. Mycek, "A UV-visible fluorescence lifetime imaging microscope for laser-based biological sensing with picosecond resolution," Appl Phys B: Lasers and Optics 76, 483-496 (2003).
13. W. Zhong, P. Urayama, and M.-A. Mycek, "Imaging fluorescence lifetime modulation of a ruthenium-based dye in living cells: the potential for oxygen sensing," J Phys D: Applied Physics 36, 1689-1695 (2003).
14. D. Lubbers, "Optical sensors for clinical monitoring," Acta Anaesthesiologica Scandinavica 39, 37-54 (1995).
15. R. P. Pandian, V. K. Kutala, N. L. Parinandi, J. L. Zweier, and P. Kuppusamy, "Measurement of oxygen consumption in mouse aortic endothelial cells using a microparticulate oximetry probe," Arch Biochem Biophys 420, 169-175 (2003).
16. J. R. Lakowicz, H. Szmacinski, K. Nowaczyk, and M. L. Johnson, "Fluorescence lifetime imaging of free and protein-bound NADH," Proceedings of the National Academy of Sciences 89, 1271-1275 (1992).
17. H. C. Gerritsen, R. Sanders, A. Draaijer, and Y. K. Levine, "Fluorescence lifetime imaging of oxygen in living cells," J Fluoresc 7, 11-16 (1997).
18. G. D. Stoner, M. E. Kaighn, R. R. Reddel, J. H. Resau, D. Bowman, Z. Naito, N. Matsukura, M. You, A. J. Galati, and C. C. Harris, "Establishment and characterization of SV40 T-antigen immortalized human esophageal epithelial cells," Cancer Research 51, 365-371 (1991).
19. O. S. Soldes, R. D. Kuick, I. A. Thompson, II, S. J. Hughes, M. B. Orringer, M. D. Iannettoni, S. M. Hanash, and D. G. Beer, "Differential expression of Hsp27 in normal oesophagus, Barrett's metaplasia and oesophageal adenocarcinomas," Br J Cancer 79, 595-603 (1999).
20. V. K. Kutala, N. L. Parinandi, R. P. Pandian, and P. Kuppusamy, "Simultaneous measurement of oxygenation in intracellular and extracellular compartments of lung microvascular endothelial cells," Antioxid Redox Signal 6, 597-603 (2004).
21. N. Khan, J. Shen, T. Y. Chang, C. C. Chang, P. C. Fung, O. Grinberg, E. Demidenko, and H. Swartz, "Plasma membrane cholesterol: a possible barrier to intracellular oxygen in normal and mutant CHO cells defective in cholesterol metabolism," Biochemistry 42, 23-29 (2003).
22. I. Georgakoudi, B. C. Jacobson, J. Van Dam, V. Backman, M. B. Wallace, M. G. Muller, Q. Zhang, K. Badizadegan, D. Sun, G. A. Thomas, L. T. Perelman, and M. S. Feld, "Fluorescence, Reflectance, and Light-Scattering Spectroscopy for Evaluating Dysplasia in patients With Barrett'e Esopghagus," Gastroenterology 120, 1620-1629 (2001).
23. R. Glasgold, M. Glasgold, H. Savage, J. Pinto, R. Alfano, and S. Schantz, "Tissue Autofluorescence as an intermediate endpoint in NMBA-induced esophageal carcinogenesis," Cancer Lett. 82, 33-41 (1994).
24. B. W. Gibson, "The human mitochondrial proteome: oxidative stress, protein modifications and oxidative phosphorylation," Int J Biochem Cell Biol 37, 927-934 (2005).
25. L. B. Chen, M. J. Welss, S. Davis, R. S. Bleday, J. R. Wong, J. Song, M. Terasaki, E. L. Shepherd, E. S. Walker, and G. D. Steele, Jr., "Mitochondria in living cells: effects of growth factors and tumor promoters, alterations in carcinoma cells, and targets for therapy," Cancer Cells 3, 433-443 (1985).
26. A. P. Brogan, W. R. Widger, and H. Kohn, "Bicyclomycin fluorescent probes: synthesis and biochemical, biophysical, and biological properties," J Org Chem 68, 5575-5587 (2003).
27. A. J. Mamelak, J. Kowalski, K. Murphy, N. Yadava, M. Zahurak, D. J. Kouba, B. G. Howell, J. Tzu, D. L. Cummins, N. J. Liegeois, K. Berg, and D. N. Sauder, "Downregulation of NDUFA1 and other oxidative phosphorylation- related genes is a consistent feature of basal cell carcinoma," Exp Dermatol 14, 336-348 (2005).
28. H. Xu, J. W. Aylott, and R. Kopelman, "Fluorescent nano-PEBBLE sensors designed for intracellular glucose imaging," Analyst 127, 1471-1477 (2002).