Imaging fluorescence lifetime modulation of a ruthenium-based dye in living cells: The potential for oxygen sensing

Journal of Physics D: Applied Physics

Volumn 36, Issue 14, 2003, Pages 1689-1695

  Zhong, Wei ^a   Urayama, Paul ^a   Mycek, Mary Ann ^a  

^a UNIVERSITY OF MICHIGAN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CAMERAS; CELLS; CHARGE COUPLED DEVICES; DYES; OXYGEN; QUENCHING; RUTHENIUM;

OXYGEN SENSING;

IMAGING TECHNIQUES;

EID: 0043201291     PISSN: 00223727     EISSN: None     Source Type: Journal    
DOI: 10.1088/0022-3727/36/14/306     Document Type: Article

References (21)

1. Rudolph W and Kempe M 1997 Topical review: trends inoptical biomedical imaging J. Modern Opt. 44 1617-42
2. Lakowicz JR 1999 Principles of Fluorescence Spectroscopy 2nd edn (New York: Kluwer Academic/Plenum)
3. Tadrous P J 2000 Methods for imaging the structure and function of living tissues and cells: 2. Fluorescence lifetime imaging J. Pathol. 191 229-34
4. Bugiel I, König K and Wabnitz H 1989 Investigation of cell by fluorescence laser scanning microscopy with subnanosecond time resolution Lasers Life Sci. 3 47-53
5. Oida T, Sako Y and Kusumi A 1993 Fluorescence lifetime imaging microscopy (filmscopy): methodology development and application to studies of endosome fusion in single cells Biophys. J. 64 676-85
6. Sanders R, Draaijer A, Gerritsen H C, Houpt P M and Levine Y K 1995 Quantitative pH imaging in cells using confocal fluorescence lifetime imaging microscopy Anal. Biochem. 227 302-08
7. Scully A D, MacRobert A J, Botchway S, O'Neill P, Parker A W, Ostler R B and Phillips D 1996 Development of a laser-based fluorescence microscope with subnanosecond time resolution J. Fluorescence 6 119-25
8. Gadella T W J Jr 1999 Fluorescence lifetime imaging microscopy (FLIM): instrumentation and application, ed W T Masons Fluorescent and Luminescent Probes for Biological Activity (San Diego: Academic) 467-79
9. Urayama P and Mycek M-A (ed) 2003 Fluorescence lifetime imaging microscopy of endogenous biological fluorescence Handbook of Biomedical Fluorescence ed M-A Mycek and B W Pogue (New York: Marcel-Dekker Inc.) 211-36
10. 2+ with animal and plant cells in vitro: mechanism of phototoxicity and conditions for non-invasive oxygen measurements J. Photochem. Photobiol. B: Biol. 65 136-44
11. Asiedu J K, Ji J, Nguyen M, Rosenzweig N and Rosenzweig Z 2001 Development of a digital fluorescence sensing technique to monitor the response of macrophages to external hypoxia J. Biomed. Opt. 6 116-21
12. Ji J, Rosenzweig N, Jones I and Rosenzweig Z 2002 Novel fluorescent oxygen indicator for intracellular oxygen measurements J. Biomed. Opt. 7 404-09
13. Gerritsen H C, Sanders R, Draaijer A and Levine Y K 1997 Fluorescence lifetime imaging of oxygen in living cells J. Fluorescence 7 11-16
14. Malak H, Dobrucki J W, Malak M M and Swartz H M 1998 Oxygen sensing in a single cell with ruthenium complexes and fluorescence time-resolve microscopy Biophys. J. 74 A189
15. Castellano F N and Lakowicz J R 1998 A water-soluble luminescence oxygen sensor Photochem. Photobiol. 67 179-83
16. Philip J and Carlsson K 2003 Theoretical investigation of the signal-to-noise ratio in fluorescence lifetime imaging J. Opt. Soc. Am. A 20 368-79
17. Urayama P, Zhong W, Beamish J A, Minn F K, Sloboda R D, Dragnev K H, Dmitrovsky E and Mycek M-A 2003 A UV-visible fluorescence lifetime imaging microscope for laser-based biological sensing with picosecond resolution Appl. Phys. B: Lasers Opt. at press
18. Ware W R, Pratinidhi M and Bauer R 1983 Performance characteristics of a small side-window photomultiplier in laser single-photon fluorescence decay measurements Rev Sci. Instrum. 54 1148-56
19. Pitts J, Sloboda R, Dragnev K, Dmitrovsky E and Mycek M-A 2001 Autofluorescence characteristics of immortalized and carcinogen-transformed human bronchial epithelial cells J. Biomedical Opt. 6 31-40
20. Webb S E D et al 2002 A wide-field time-domain fluorescence lifetime imaging microscope with optical sectioning Rev. Sci. Instrum. 73 1898-907
21. Chance B, Schoener B, Oshino R, Itshak F and Nakase Y 1979 Oxidation-reduction ratio studies of mitochondria in freeze-trapped samples J. Biol. Chem. 254 4764-71