Dopamine uptake and cocaine binding mechanisms: The involvement of charged amino acids from the transmembrane domains of the human dopamine transporter

European Journal of Pharmacology

Volumn 538, Issue 1-3, 2006, Pages 43-47

  Dar, Dalit E ^a   Metzger, Thomas G ^a   Vandenbergh, David J ^a   Uhl, George R ^a  

^a NATIONAL INSTITUTE ON DRUG ABUSE   (United States)

Author keywords

Charged amino acid; Cocaine; Dopamine transporter; Glutamate; Mutant

Indexed keywords

2 BETA CARBOMETHOXY 3 BETA (4 FLUOROPHENYL)TROPANE; AMINO ACID; ARGININE 283; ARGININE 521; COCAINE; COCAINE DERIVATIVE; DOPAMINE TRANSPORTER; GLUTAMATE 491; LYSINE 257; LYSINE 525; MUTANT PROTEIN; RADIOACTIVE MATERIAL; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ANIMAL CELL; ARTICLE; BINDING KINETICS; CELL STRAIN COS7; CONTROLLED STUDY; DOPAMINE UPTAKE; GENETIC TRANSFECTION; HYDROPHOBICITY; IC 50; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN;

ALANINE; AMINO ACID SUBSTITUTION; ANIMALS; ARGININE; BINDING SITES; BINDING, COMPETITIVE; CERCOPITHECUS AETHIOPS; COCAINE; COS CELLS; DOPAMINE; DOPAMINE PLASMA MEMBRANE TRANSPORT PROTEINS; GLUTAMIC ACID; HUMANS; KINETICS; LYSINE; MUTATION; TRANSFECTION; TRITIUM;

EID: 33646529365     PISSN: 00142999     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ejphar.2006.03.048     Document Type: Article

References (20)

1. Chen N., and Reith M.E. Structure and function of the dopamine transporter. Eur. J. Pharmacol. 405 (2000) 329-339
2. 3H]WIN 35,428 binding to the dopamine uptake carrier. I. Effect of tonicity and buffer composition. J. Neurosci. Methods 51 (1994) 23-30
3. Dar D.E., Mayo C., and Uhl G.R. The interaction of methylphenidate and benztropine with the dopamine transporter is different than other substrates and ligands. Biochem. Pharmacol. 70 (2005) 461-469
4. Giros B., Mestikawy S.E., Godinot N., Zheng K., Han H., Yang-Feng T., and Caron M. Cloning, pharmacological characterization, and chromosome assignment of the human dopamine transporter. Mol. Pharmacol. 42 (1992) 383-390
5. Goldberg N.R., Beuming T., Soyer O.S., Goldstein R.A., Weinstein H., and Javitch J.A. Probing conformational changes in neurotransmitter transporters: a structural context. Eur. J. Pharmacol. 479 (2003) 3-12
6. Itokawa M., Lin Z., Cai N.S., Wu C., Kitayama S., Wang J.B., and Uhl G.R. Dopamine transporter transmembrane domain polar mutants: DeltaG and DeltaDeltaG values implicate regions important for transporter function. Mol. Pharmacol. 57 (2000) 1093-1103
7. Kitayama S., Shimada S., Xu M., Markham L., Donovan D.M., and Uhl G.R. Dopamine transporter site-directed mutations differentially alter substrate transport and cocaine binding. Proc. Natl. Acad. Sci. 89 (1992) 7782-7785
8. + transport. Synapse 15 (1993) 58-62
9. Kuhar M.J., Ritz M.C., and Boja J. The dopamine hypothesis of reinforcing properties of cocaine. Trends Neurosci. 14 (1991) 299-302
10. Lin Z., Wang W., Kopajtic T., Revay R.S., and Uhl G.R. Dopamine transporter: transmembrane phenylalanine mutations can selectively influence dopamine uptake and cocaine analog recognition. Mol. Pharmacol. 56 (1999) 434-447
11. Lin Z., Wang W., and Uhl G.R. Dopamine transporter tryptophan mutants highlight candidate dopamine and cocaine selective domains. Mol. Pharmacol. 58 (2000) 1581-1592
12. Lin Z., Itokawa M., and Uhl G.R. Dopamine transporter proline mutation influences dopamine uptake, cocaine analog recognition and expression. FASEB J. 14 (2000) 715-728
13. Norregaard L., and Gether U. The monoamines neurotransmitter transporters: structure, conformational changes and molecular gating. Curr. Opin. Drug Discov. Dev. 4 (2001) 591-601
14. Pacholczyk T., Blakely R.D., and Amara S.G. Expression cloning of a cocaine- and antidepressant-sensitive human noradrenaline transporter. Nature 350 (1991) 350-353
15. 3H]GBR 12,935 binding. Mol. Pharmacol. 45 (1994) 125-135
16. Ritz M.C., Lamb R.J., Goldberg S.R., and Kuhar M.J. Cocaine receptors on dopamine transporters are related to self-administration of cocaine. Science 273 (1987) 1219-1223
17. Schaeffer J.C., Lin C.L., Kitayama S., and Uhl G.R. Ligand autoradiographic receptor screening. II. Expression of receptor cDNA in transfected COS cells grown on polyester disks and its recovery. Brain Res. Mol. Brain Res. 9 (1991) 271-276
18. Shimada S., Kitayama S., Lin C.C., Patel A., Nanthakumar E., Gregor P., Kuhar M., and Uhl G.R. Cloning and expression of a cocaine-sensitive dopamine transporter complementary DNA. Science 254 (1991) 576-578
19. Vandenbergh D.J., Persico A.M., and Uhl G.R. A human dopamine transporter cDNA predicts reduced glycosylation, displays a novel repetitive element and provides racially-dimorphic TaqI RFLPs. Brain Res. Mol. Brain Res. 15 (1992) 161-166
20. - dependent neurotransmitter transporters. Nature 437 (2005) 215-223